日本老化Jaica-Anti thymidineglycol (TG) monoclonal antibody


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Anti thymidineglycol (TG) monoclonal antibody Anti thymidineglycol (TG) monoclonal antibody
Anti thymidineglycol (TG) monoclonal antibody
Suitable for immunohistocheistry. For research use only.
[ Specific to DNA oxidation ]
Anti thymidineglycol (TG) monoclonal antibody Thymidineglycol (TG) is one of the major oxidation products of DNA. Thymidine (T) can be damaged by oxidative stress such as radiation and energy metabolism. Two different pathways to form TG have been suggested. Deoxythymidine in DNA is directly oxidised by hydroxy radical, to form TG. TG can be also formed through an intermediate thymidine chlorohydrin, which is derived from hypochlorous acid (HOCl) from neutrophil myeloperoxidase. Thymidineglycol is derived from DNA, not from RNA. TG is the oxidative stress marker secific for DNA damage.
[ Comparison with 8-OHdG ]
8-hydroxy-2′-deoxyguanosine (8-OHdG) is one of the well-known DNA oxidation products. In an lipopolysaccharide (LPS)-treated mouse liver, 8-OHdG can be stained in 24 hours after LPS treatment. TG can be detected in 6 hours, and remain at least for 72 hours. Anti TG monoclonal antibody (clone 2E8) has been established by Dr. Toshihiko Osawa (Nagoya Univ.). Poly dT (50 mer) has been oxidised by OsO4 and injected to Balb/c mouse as immunogen. This antibody can react with thymidineglycol in DNA or TG polymer, and suitable for immunohistochemistry.
Anti thymidineglycol (TG) monoclonal antibody
Anti thymidineglycol (TG) monoclonal antibody
[ Specifications ]
Antigen: Thymidineglycol polymer.
Form: Lyophilized power. Reconstitute by 1000 µL of distilled water. 100 µg/mL of IgG.
Subclass: Mouse IgG1 (kappa), clone 2E8
Specificity: Specific for DNA fragment containing thymidine glycol, and thymidine glycol polymer.
Cross reactivity have been tested to following analogues: oxidized deoxycytidine polymer, oxidized deoxyguanosine polymer and oxidized deoxyadenosine polymer. Not react with free thymidineglycol.
Application: Immunohistochemistry (antibody conc. 5 – 10 µg/mL)
Storage: Less than -20°C
Anti thymidineglycol (TG) monoclonal antibody
Reference
1) Ito K, Yano T, Morodomi Y, Yoshida T, Kohno M, Haro A, Shikada Y, Okamoto T, Maruyama R, Maehara Y.
Serum antioxidant capacity and oxidative injury to pulmonary DNA in never-smokers with primary lung cancer.
Anticancer Res. 32(3),p1063-1067(2012)
Anti thymidineglycol (TG) monoclonal antibody
Product name Code Content
Anti thymidineglycol (TG) monoclonal antibody MTG-100P 100 µg IgG
Anti thymidineglycol (TG) monoclonal antibody

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Anti thymidineglycol (TG) monoclonal antibody
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日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒,并且处于该领域的领先地位。JaICA氧化应激相关产品:DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析,以及微量元素分析。详情请咨询JaICA中国区代理商:上海金畔生物科技有限公司。