MF-registered Small Molecules
The products are registered in the Drug Master File (MF) in Japan as additives for culture medium.
We ensure high stability and quality of the products by validating the manufacturing and analytical processes and implementing change control strategies.
GMP products comming soon!
FUJIFILM Wako will provide the small molecules produced through GMP based on ICHQ7 in 2021.
- Y-27632, MF
- A-83-01, MF
- CHIR99021, MF
- SB431542, MF
- Note
- Product List
- Related Product List
- Related Information
Y-27632, MF
ROCK inhibitor
Y-27632 improves the viability and cloning efficiency of human ES/ iPS cells after cryopreservation. Furthermore, Y-27632 can be used with EGF, CHIR99021, A-83-01, and SB431542 to establish Trophoblast stem (TS) cells from human blastocysts.
<Product Description>
- Appearance: white to pale yellow, crystalline powder to powder
- Assay (HPLC): min. 98.0%
- Solubility: soluble in water
- Specific rotation [α]D20(c=1.0,CH3OH) :+2.0°~+10.0°
- Endotoxin※: less than 0.25 EU/mg
- Tested negative for mycoplasma※
- Tested for viable bacteria※
- CAS RN®: 331752-47-7
- C14H21N3O・2HCl・H2O=338.27
- [Registration number: 227MF40013]
References
- Uehata, M., et al. : Nature, 389, 990 (1997).
- Sakamoto, K., et al. : J. Pharmacol. Sci., 92, 56 (2003).
- Nishimaru, K., et al. : J. Pharmacol. Sci., 92, 424 (2003).
- Watanabe, K., et al. : Nat. Biotechnol., 25, 681 (2007).
- Martin-Ibanez, R., et al. : Hum. Reprod., 23, 2744 (2008).
- Claassen, DA., et al. : Mol. Reprod. Dev., 76, 722 (2009).
- Kawamata, M., et al. : Proc. Natl. Acad. Sci. USA., 107, 14223 (2010).
- Ito, H., et al. : Liver Int., 32, 592 (2012).
- Katsuda,T., et al. : Cell Stem Cell, 20, 41 (2017).
- Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
- Ogawa, K., et al. : Sci. Rep., 8, 3615 (2018).
- Ayabe, H., et al. : Stem Cell Reports., 11, 306 (2018).
A-83-01, MF
Inhibitor of ALK4, ALK5, and ALK7
A-83-01 enables long-term and consistent culture of rat and canine iPS cells in a state maintaining the undifferentiated state.. Furthermore, the use of A-83-01 with Y-27632 and CHIR99021 induces reprogramming of rat and mouse mature hepatocytes into liver progenitors that can be cultured stably in vitro
<Product Description>
- Appearance: white to yellow, crystalline powder to powder or mass
- Assay (HPLC): min. 98.0%
- Solubility: soluble in DMSO
- Endotoxin※: less than 0.01 EU/mg
- Tested negative for mycoplasma※
- Tested for viable bacteria※
- CAS RN®: 909910-43-6
- C25H19N5S=421.52
- [Resistration number: 229MF40005]
References
- Li, W., et al. : Cell Stem Cell, 4, 16 (2009).
- Katsuda ,T., et al. : Cell Stem Cell, 20, 41 (2017).
- Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
CHIR99021, MF
GSK-3β Inhibitor
It has been reported that differentiation can be suppressed with high efficiency when ES cells are cultured in a medium containing CHIR99021 and PD0325901.
Product Description
- Appearance: white~pale brown, crystalline powder~powder
- Assay (HPLC): min. 98.0%
- Solubility: soluble in methanol
- Water: max. 1.0%
- Endotoxin※: less than 0.05EU/mg
- Tested negative for mycoplasma※
- Tested for viable bacteria※
- CAS RN®: 252917-06-9
- C22H18Cl2N8 = 465.34
- Registration number: 231MF40003
References
- Ying, QL., et al.: Nature, 453, 519 (2008).
- Fujimori, K., et al.: Stem Cell Reports., 9, 1675 (2017).
SB431542, MF
Inhibitor of ALK4, ALK5, and ALK7
SB431542 promotes proliferation, differentiation and sheet formation of ES cells-derived endothelial cells. It also improves reprogramming efficiency and speed when used with PD0325901 and thiazovivin. It has also been reported that cells induced by SB43154, CHIR99021, and dorsomorphin have significantly improved differentiation efficiency and speed.
<Product Description>
- Appearance: white to yellowish brown, crystalline powder to powder
- Assay(HPLC): min. 98.0%
- Solubility: soluble in DMSO
- Endotoxin※: less than 0.05 EU/mg
- Tested negative for mycoplasma※
- Tested for viable bacteria※
- CAS RN®: 301836-41-9
- C22H16N4O3=384.39
- [Resistration number: 228MF40016]
References
- Ogawa, K., et al. : J. Cell. Sci.,120, 55 (2007).
- Lin T., et al. : Nat. Methods, 6, 805 (2009).
- Fujimori, K., et al. : Stem Cell Reports., 9, 1 (2017).
- Okae, H., et al. : Cell Stem Cell, 22, 50 (2018).
Note
- Tests for mycoplasma, endotoxin, and viable bacteria are performed for every lot as part of standard tests; however, the results are not required for MF registration.
- Registration in the Drug Master File (MF) does not indicate that the product was tested for its quality and safety by the Ministry of Health, Labor and Welfare (Pharmaceuticals and Medical Devices Agency).
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Regenerative medicine product material qualification consulted product
Related Information
Category
- Cell Culture
- Stem Cell Culture
- Undifferentiated State Maintenance / Differentiation Induction
- Activin A Solution, Human, Recombinant
- Animal-free Cytokines
- Animal-free Cytokines
- Y-27632, MF
- CultureSure® Small Molecules
- StemSure® hPSC MediumΔ
- Small Molecules for ES/iPS cells Research
Product content may differ from the actual image due to minor specification changes etc.