Wako和光纯药Vero 细胞培养基Vero Cell Culture Medium

Wako和光纯药Vero 细胞培养基Vero Cell Culture Medium

Vero Cell Culture Medium

FUJIFILM Wako promotes our proprietary media product lines under WakoVAC, manufactured under GMP-compliant system and scalable to commercial volumes.
We are increasing the variety of prototypes available for our library of WakoVAC Vero, and we can provide you samples for testing.

Media Optimization Service
  • Brochure
  • Features
  • Performance
  • Media Optimization Service
  • Product List
  • Related Product List
  • Related Information

Features

  • Animal component-free
  • Designed and optimized for small- and large-scale vaccine production
  • Scalable, adaptable system to support for Commercialization
  • GMP available upon request

Performance

WakoVAC Vero prototypes A and B (media 4 and 5 on the graph) have shown relatively high performances growing larger number of cells under the conditions below.

  • Plate Corning 12 well Plate (Corning, #3513)
    Cell type Vero cell
    Number of cells plated 30000 cells/well
    Condition 37℃, 5%CO2
    Culturing duration 5 days
    Media used 2.0 mL
  • Vero Cell Culture Medium
  • 1: EMEM/5%FBS
    2: DMEM/5%FBS
    3: SFM from a competitor
    4: WakoVAC Vero prototype A
    5: WakoVAC Vero prototype B

Media Optimization Service

WakoVAC Vero will be provided as a made-to-order product, and FUJIFILM Wako is now increasing the variety of prototypes.

Service Flow

  • 1. Inquiry
  • 2. Making test sample at FUJIFILM Wako
  • 3. Sample testing at your site
  • 4. Feedback of the test result
  • 5. Customization
  • 6. Fix formulation
  • 7. Place order
  • 8. Ship the media
Note:
-We respond to flexible small scale media demand with non-GMP samples formulated with the same quality raw materials used in GMP level. COS (testing includes appearance, pH and osmo) is available upon request.
-Non-disclosure agreement may be necessary prior to discussing potential business coming forward.
-The shipping cost of the sample will be decided after consultation.
Media Optimization Service

Product List

This is a made-to-order product. Please visit Media Optimization Service.

这是定制产品。 详情请咨询。

Related Product List   其他相关产品

Trypsin-EDTA Solution without Phenol Red, AF

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
203-20251
100mL
List Price
JPY 9,000
205-20255
500mL
List Price
JPY 24,000

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CultureSure® Freezing Medium

for Cell Culture

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
039-23511
100mL
List Price
JPY 8,300

In stock in Japan

Related Information

Category

  1. Cell Culture
  2. Vaccine Manufacturing
  3. Medium

Product content may differ from the actual image due to minor specification changes etc.

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako 011-20171 Azoxymethane 氧化偶氮甲烷(AOM)25843-45-2

Wako 011-20171 Azoxymethane 氧化偶氮甲烷 25843-45-2

氧化偶氮甲烷 Azoxymethane(AOM)

细胞生物学用 for Cellbiology

0616099198374382.jpg

纯度 : 95.0+% (Capillary GC)

制造商 : FUJIFILM Wako Pure Chemical Corporation(富士胶片和光)

储存条件:冷藏(干冰运输)

CAS RN® : 25843-45-2

分子式 : C2H6N2O

分子量 : 74.08

◆描述/应用例

本产品可强效诱发小鼠、大鼠的结肠癌,作为建立结肠癌模型的试剂而广泛用于癌症预防物质的探索以及癌症引发机理的研究。随着对结肠癌的担忧不断增加,人们越发积极地探索癌症预防物质,因此本产品所起的作用非常重要。本产品的平均给药量为每周一次皮下注射大鼠15 mg/kg(体重),持续约3周,随后检测到异常隐窝灶(ACF)。

建立病理动物模型用试剂

使用病理模型动物是研究各种疾病的重要研究方法之一,通过向大鼠、小鼠等动物施用化学物质,可建立表现人类疾病特征性质的病理动物模型。

相关疾病:大肠癌

用途

致癌诱发物质、大肠癌诱发剂。

使用方法

每周一次皮下注射大鼠15 mg /kg,持续2~3周,之后约第3周被检测到异常隐窝灶(ACF)。

注意事项: 充氩包装

物性信息

外观:液体

溶解性:溶于乙醇和丙酮,在水中可被分解。

比重:0.991

产品编号 产品名称 产品规格 产品等级
011-20171 Azoxymethane (AOM)
氧化偶氮甲烷
100 mg 细胞生物学用

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako 011-19365 偶氮二异丙基咪唑啉盐[VA-044]27776-21-2

Wako 011-19365 偶氮二异丙基咪唑啉盐[VA-044]27776-21-2

VA-044    2,2′-Azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride

017-19362

中文名称:偶氮二异丁咪唑啉盐酸盐
CAS NO.:27776-21-2
英文缩写:AIPC / AIBI

◆特征 

● 非腈、低温型水溶性偶氮重合开始剂
●  可溶于水、甲醇
● 10小时半减期温度44℃(水中)

◆化学名

2,2′-Azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride

VA044.jpg

 

CAS NO. 27776-21-2

分子量 = 323.27

◆物理性质

外观:白色~淡黄色 ,结晶或者结晶型粉末

融点:188~193℃(分解)

10小时半减期温度:44℃(水中)

活化性能量:108.2K J/mol    K

频率因子(ln A):30.19

溶解性:

水:易溶
甲醇:有点难溶
乙醇:非常难溶
丙酮、甲苯

SADT:140℃

毒性(LG50):3,200 mg/kg(经口大鼠[雄])
2,800 mg/kg(经口大鼠[雌])

◆包装、保存条件 

20 kg(4×5 kg)纸箱
500 g 纸盒

30℃以下保存

◆相关法规·安全性

剧毒法 不符合
消防法 不符合
化审法 5-5870、1-215
安卫法 已存在
TSCA Listed
EINECS 248-655-3
产品编号 产品名称 产品规格 产品等级
017-19362 2,2′-Azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride
偶氮二异丁咪唑啉盐酸盐
25 g
011-19365 2,2′-Azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride
偶氮二异丁咪唑啉盐酸盐
500 g

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako和光纯药 MDCK 细胞培养基 MDCK Cell Culture Medium

Wako和光纯药 MDCK 细胞培养基 MDCK Cell Culture Medium

MDCK Cell Culture Medium

FUJIFILM Wako promotes our proprietary media product lines under WakoVAC, manufactured under GMP-compliant system and scalable to commercial volumes.
WakoVAC MDCK is designed to maximize the growth of MDCK cell and its virus production, and built to support for large manufacturing.

Wako Proprietary Media

Products developed under WakoVAC brand is taking a comprehensive approach to meet the key requirements for cell culture products in the field of life science. WakoVAC MDCK is targeted to support optimal and stable expansion of MDCK cells while retaining viability and target protein expression.

Raw Material Supply

Animal components free raw materials are used in WakoVAC MDCK. All raw materials are documented and traceable. All raw materials used are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC MDCK contains yeast-derived Hydrolysate.

原材料供应

WakoVAC MDCK 使用不含动物成分的原材料。所有原材料都有记录和可追溯。所有使用的原材料都按照特定规格标准化,可扩展,并由经过验证的供应商提供。WakoVAC MDCK 含有酵母来源的水解物。

Industrial Applications

The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.

Performance

WakoVAC MDCK includes multiple prototypes formulated to support MDCK cell types. It is designed to maximize and consistent MDCK cell growth, viability and sustains high productivity.

Media Optimization Service
  • Brochure
  • Features
  • Specification
  • Cell culture and target expression
  • Media Optimization Service
  • Product List
  • Related Product List
  • Related Information

Features

  • Animal component-free
  • Multiple prototypes are available for testing
  • The latest prototype shows an improved virus yield approximately 1.5 times higher than competitor’s Medium A, commercially available serum-free media
  • Designed and optimized for small- and large-scale vaccine production
  • Compatible with microcarrier
  • Adaptation not typically required
  • Scalable and adaptable system to support for commercialization
  • GMP compliant manufactured

Specification

PN TBD
Format Powder (and Liquid upon request)
Shelf-life TBD
Storage 2-10˚C, protect from light
Standard Packaging Size 8kg (Powder), 500mL / 1L (Liquid)
*Flexible packaging is available upon request.

Intended Use: For Research and Further Manufacturing

Cell culture and target expression

WakoVAC MDCK outperforms FBS contained alternative

MDCK Cell Culture Medium

Equal to or higher yields of influenza virus than commercially available serum-containing option

MDCK Cell Culture Medium

Adaptation is not necessarily required

  • Switch from a commercially available serum-free medium
    MDCK Cell Culture Medium
  • Switch from a serum-containing medium
    MDCK Cell Culture Medium

Compatible with microcarrier

  • MDCK Cell Culture Medium
  • MDCK Cell Culture Medium

Media Optimization Service

This is a made-to-order product. Please fill out your information at Media Optimization Service.

Service Flow

  • 1. Inquiry
  • 2. Making test sample at FUJIFILM Wako
  • 3. Sample testing at your site
  • 4. Feedback of the test result
  • 5. Customization
  • 6. Fix formulation
  • 7. Place order
  • 8. Ship the media
Note:
-We respond to flexible small scale media demand with non-GMP samples formulated with the same quality raw materials used in GMP level. COS (testing includes appearance, pH and osmo) is available upon request.
-Non-disclosure agreement may be necessary prior to discussing potential business coming forward.
-The shipping cost of the sample will be decided after consultation.
Media Optimization Service

Product List

This is a made-to-order product. Please visit Media Optimization Service.

Related Product List

  • Trypsin-EDTA Solution without Phenol Red, AF

    for Cell Culture

    Manufacturer : FUJIFILM Wako Pure Chemical Corporation
    Storage Condition : Keep at -20 degrees C.
    Comparison
    Product Number
    Package Size
    Price
    Inventory
    203-20251
    100mL
    List Price
    JPY 9,000
    205-20255
    500mL
    List Price
    JPY 24,000

    In stock in Japan

    CultureSure® Freezing Medium

    for Cell Culture

    Manufacturer : FUJIFILM Wako Pure Chemical Corporation
    Storage Condition : Keep at 2-10 degrees C.
    Comparison
    Product Number
    Package Size
    Price
    Inventory
    Distributor
    039-23511
    100mL
    List Price
    JPY 8,300

    In stock in Japan

Related Information

Category

  1. Cell Culture
  2. Vaccine Manufacturing
  3. Medium

Product content may differ from the actual image due to minor specification changes etc.

Wako和光纯药 昆虫细胞培养基Insect Cell Culture Medium: WakoVAC PSFM-J1

Wako和光纯药 昆虫细胞培养基Insect Cell Culture Medium: WakoVAC PSFM-J1

Insect Cell Culture Medium: WakoVAC PSFM-J1

Licensed Formulation

Formulated for insect cell expansion. Designed to maximize insect cell growth, viability and productivity. WakoVAC PSFM-J1 manufactured in Wako outperforms the same formulation manufactured in competitor’s site due to using quality raw materials and strict and robust raw material control.

Raw Material

All raw materials are documented and traceable. All raw materials used in WakoVAC PSFM-J1 are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC PSFM-J1 contains fish-derived component (cGMP, virus-inactivated) and Hydorlysate. Without fish-derived component, the media can be defined animal component-free.

Industrial Applications

The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.

 

Performance

WakoVAC PSJF-J1 supports a wide range of insect cells: Sf+, Hi5, Sf9, Sf21. It is designed to maximize and consistent insect cell growth, viability and sustains high productivity. Best in class performance, Easy to use, Ongoing stability.

许可配方

专为昆虫细胞扩增而配制。旨在最大限度地提高昆虫细胞的生长、活力和生产力。由于使用优质原材料和严格而稳健的原材料控制,Wako 制造的 WakoVAC PSFM-J1 的性能优于竞争对手现场制造的相同配方。

原材料

所有原材料都有记录和可追溯。WakoVAC PSFM-J1 中使用的所有原材料均已标准化为特定规格、可扩展,并由经过验证的供应商提供。WakoVAC PSFM-J1 含有鱼源成分(cGMP,病毒灭活)和水解物。没有鱼源成分,培养基可以定义为不含动物成分。

工业应用

WakoVAC(符合 GMP 制造)的预期用途是用于研究、工艺开发、大规模制造/商业化。WakoVAC 可扩展到工业应用的商业量。液体和粉末都可用。也可应要求提供软包装。Wako 在 IND/NDA 时提供从媒体优化服务到监管支持的完整工作流程支持。

表现

WakoVAC PSJF-J1 支持多种昆虫细胞:Sf+、Hi5、Sf9、Sf21。它旨在最大限度地和一致的昆虫细胞生长、活力和维持高生产力。一流的性能,易于使用,持续的稳定性。

  • Brochure
  • Features
  • Culturing Sf9 Cells
  • Culturing Hi5 Cells
  • Target expression data with Sf21
  • Product List
  • Related Product List
  • Related Information

Features

  • Support wide range of insect cells
  • Performs best with Sf+
    • Cell GrowthInsect Cell Culture Medium: WakoVAC PSFM-J1
    • Cell ViabilityInsect Cell Culture Medium: WakoVAC PSFM-J1

     

    Insect Cell Culture Medium: WakoVAC PSFM-J1

  • Fish-derived component(cGMP) and hydrolysate contained
  • CoA for non-GMP and GMP available
    Cell growth, cell viability, and protein expression data can be included on CoA upon request
  • Intended use: for research and further manufacturing
Insect Cell Culture Medium: WakoVAC PSFM-J1
Wako Cat# 160-25851 TBD
Format Liquid Powder
Package 1L 8kg and/or Customs upon request
Shelf-life 1 year from the date of MFG 2 years from the date of MFG
Storage Condition 2-10˚C, protect from light

Other Information

  • Assure lot-to-lot consistency
  • Fine protein expression as well with Sf9, Sf21 and Hi5
  • L-Glutamine does not need to be supplemented
  • Comparative Landscape
    Product Description Supplier SF ACF PF CD GMP Hydrolysate Cell Type Shelf-life Storage Size Bag Avaiability
    Sf Insect FUJIFILM Irvine Scientific Yes Yes Yes + Sf9, Sf21 1 year 2-8℃ 1L 5L(MTO) Custom upon request
    WakoVAC PSFM-J1 FUJIFILM Wako Yes Fish-derived component Yes Yes ++ Sf+, Hi5, Sf9, and Sf21 1 year 2-10℃ 1L 10L and 20L Powder available

Culturing Sf9 Cells

Retain robust cell growth, viability and target expression with Sf9

Cell Growth
Insect Cell Culture Medium: WakoVAC PSFM-J1
Protein Expression Testing
Insect Cell Culture Medium: WakoVAC PSFM-J1

M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Medium B Control
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody

* Data provided by the Goshima Lab from Nagoya University

Cell Viability
Insect Cell Culture Medium: WakoVAC PSFM-J1

Culturing Hi5 Cells

Retain robust cell growth, viability and target expression with Hi5

Cell Growth
Insect Cell Culture Medium: WakoVAC PSFM-J1
Protein Expression Testing
Insect Cell Culture Medium: WakoVAC PSFM-J1

M: Molecular weight size marker
Lane 1: Wako VAC PSFM-J1
Lane 2: Wako VAC PSFM-J1 + L-Glutamine
Lane 3: Medium C Control + L-Glutamine
: Target protein
  • The amount of samples being used: 20μl/Lane
  • SDS-PAGE: SuperSep™ Ace 10-20% (Wako Code: 191-15031),
    200v constant voltage for 60 minutes
  • Western blotting: PVDF membrane
  • Antibody: Anti- his Tag antibody

* Data provided by Mirai-Kougaku

Cell Viability
Insect Cell Culture Medium: WakoVAC PSFM-J1

Target expression data with Sf21

Retain target expression with Sf21

Protein Expression Testing

Insect Cell Culture Medium: WakoVAC PSFM-J1

M: Molecular weight size marker
Lane 1: Medium A (Pre-viral inoculation)
Lane 2: Medium A (Post-viral inoculation)
Lane 3: PSFM-J1 (Pre-viral inoculation)
Lane 4: PSFM-J1 (Post-viral inoculation)
: Target protein
  • The amount of samples being used: 15μl/Lane
  • SDS-PAGE: 5-20% gradient gel, 200v constant
  • Voltage for 50 minutes

* Data provided by the Goshima Lab from Nagoya University

Product List

  • PSFM-J1 Medium Wako, Liquid

    for Cell Culture

    Manufacturer : FUJIFILM Wako Pure Chemical Corporation
    Storage Condition : Keep at 2-10 degrees C.
    Comparison
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    Package Size
    Price
    Inventory
    160-25851
    1L
    List Price
    JPY 7,300

    In stock in Japan

    162-25855
    500mL
    Discontinued

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Cholesterol, Semi-synthetic Discontinued

for Cell Culture

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
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034-22961
5G
Discontinued
Distributor
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25G
Discontinued

Polyoxyethylene Polyoxypropylene Glycol (160E.O.) (30P.O.)

for Biochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
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160-25231
100G
List Price
JPY 5,100

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Distributor
162-25235
500G
List Price
JPY 13,600

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Polyacrylamide gel SuperSep™ Ace

SuperSep (TM) Ace, 7.5%, 13well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
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Package Size
Price
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Distributor
198-14941
10EA
List Price
JPY 14,000

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SuperSep (TM) Ace, 7.5%, 17well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
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Package Size
Price
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Distributor
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List Price
JPY 14,000

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SuperSep (TM) Ace, 10%, 13well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
195-14951
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 10%, 17well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
192-14961
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 12.5%, 13well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
199-14971
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 12.5%, 17well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
196-14981
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 15%, 13well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
193-14991
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 15%, 17well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
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Distributor
190-15001
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 5-20%, 13well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
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SuperSep (TM) Ace, 5-20%, 17well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
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Price
Inventory
Distributor
194-15021
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 10-20%, 13well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
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Package Size
Price
Inventory
Distributor
191-15031
10EA
List Price
JPY 14,000

In stock in Japan

SuperSep (TM) Ace, 10-20%, 17well

for Electrophoresis

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
198-15041
10EA
List Price
JPY 14,000

In stock in Japan

Polyacrylamide gel SuperSep™ Ace

Related Information

Category

  1. Cell Culture
  2. Insect Cell Culture
  3. Medium
  1. Cell Culture
  2. Vaccine Manufacturing
  3. Medium

Product content may differ from the actual image due to minor specification changes etc.

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako 011-09811 Aphidicolin 阿非迪霉素 38966-21-1

Wako 011-09811 Aphidicolin 阿非迪霉素 38966-21-1

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako和光纯药 Lysyl Endopeptidase 赖氨酰内肽酶

Wako和光纯药 Lysyl Endopeptidase 赖氨酰内肽酶

Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)

This product is a recombinant Lysyl Endopeptidase® expressed in E. coli. Lysyl endopeptidase® is a serine protease that cleaves peptide bonds at the carboxy-terminus of lysine residues with high specificity.
Taking advantage of its excellent specificity, Lysyl Endopeptidase® is used for peptide fragmentation and peptide mapping for analysis of the primary structure of proteins. This product is checked residual DNA and Host cell protein for biopharmaceutical analysis.

赖氨酰内肽酶®,重组,生物药物分析级 (rLys-C)

该产品是在大肠杆菌中表达的重组赖氨酰内肽酶®。赖氨酰内肽酶®是一种丝氨酸蛋白酶,可高度特异性地切割赖氨酸残基羧基末端的肽键。Lysyl Endopeptidase ®利用其出色的特异性,可用于肽片段化和肽图分析,以分析蛋白质的一级结构。本产品经检查残留 DNA 和宿主细胞蛋白,用于生物制药分析。

  • Brochure
  • Biochemistry Grade
    Lysyl Endopeptidase®
  • Mass Spectrometry Grade
    Lysyl Endopeptidase®
  • Features
  • Specification
  • BSA Digestion Analysis
  • Procedure
  • Product List
  • Related Product List
  • Related Information

Features

  • Expressed in E. coli
  • Checked residual DNA / Host cell protein
  • Missed cleavage rate is lower than competitor‘s Lys-C

Specification

Requirement Specification
Appearance Lyophilisate
Activity ≧2.0AU/mg
DNA residual test ≦10ng/mg
HCP assay ≦1.0μg/mg
Endotoxin testing <20EU/mg
Electrophoresis test (SDS-PAGE) to pass test

规格

要求 规格
外貌 冻干物
活动 ≧2.0AU/mg
DNA残留检测 ≤10ng/mg
HCP测定 ≤1.0μg/mg
内毒素检测 <20EU/mg
电泳测试(SDS-PAGE) 通过测试

BSA Digestion Analysis

We incubated BSA with each protease for 1 hour and 18 hours.
After incubations, we analized the missed cleavage rate and number of peptides.

Our Product Competitor’s Product
Specificity
(Missed cleavage rate after 1 hour’s incubation)
0% 0%
Specificity
(Missed cleavage rate after 18 hours’ incubation)
10% 20%
Activity
(Number of peptides after 18 hours’ incubation)
41 35

The data suggested that our rLys-C have higher activity and specificity in long time reaction than the competitor’s product.

Procedure

1. Sample preparation

①Dissolve or dilute the protein sample to be digested with 25 mmol/L Tris-HCl, 1 mmol/L EDTA,pH 8.5~9.0.
②Add Disulfide threitositol (DTT) or β-mercaptoethanol to the solubilized protein at the final concentration of 5 mmol/L.
③Incubate for 30 min at room temperature.
④Add iodoacetamide to the solubilized protein at the final concentration of 10 mmol/L.
⑤Incubate in dark for 30 min at room temperature.

2. rLys-C preparation

①Dissolve lyophilized powder of rLys-C with 100 μl 12.5 mmol/L Tris-HCl, pH 8.5~9.0.

3. Sample digestion

①Add the prepared rLys-C to sample solution according to the enzyme : protein mass ratio of 1:20~1:100, and incubate at 35℃~37℃ for 2~18 hours.
②The final concentration of 0.5~1.0v/v% trifluoroacetic acid is added to stop the reaction.

程序

1. 样品制备

①用25 mmol/L Tris-HCl,1 mmol/L EDTA,pH 8.5~9.0溶解或稀释待消化的蛋白质样品。
②在溶解的蛋白质中加入二硫化物苏糖醇(DTT)或β-巯基乙醇至终浓度为5 mmol/L。
③室温孵育30分钟。
④在溶解的蛋白质中加入碘乙酰胺,终浓度为10 mmol/L。
⑤室温避光孵育30分钟。

2. rLys-C 的制备

①用100 μl 12.5 mmol/L Tris-HCl,pH 8.5~9.0溶解rLys-C冻干粉。

3. 样品消解

①将配制好的rLys-C按酶:蛋白质量比1:20~1:100加入样品溶液,35℃~37℃孵育2~18小时。
②加入终浓度0.5~1.0v/v%的三氟乙酸终止反应。

Product List

Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)

for Cell Culture

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
124-06871
20ug

Related Product List

Biochemistry Grade

Lysyl EndopeptidaseR (Lys-C)

for Biochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Inventory
Distributor
129-02541
1vial(10AU)
Inquire

In stock in Japan

Distributor
125-02543
1vial(2AU)
Inquire

In stock in Japan

Mass spectrometry grade

Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)

for Proteome Research

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
125-05061
20ug x5
List Price
JPY 75,000

In stock in Japan

Distributor
121-05063
20ug
List Price
JPY 20,000

In stock in Japan

Biochemistry Grade

Mass spectrometry grade

Related Information

Category

  1. Life Science
  2. Enzyme
  3. Protease
  1. Cell Culture
  2. Biopharmaceuticals Manufacturing
  3. Quality Control Test

Product content may differ from the actual image due to minor specification changes etc.

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako 011-03211 Hexaammonium Heptamolybdate Tetrahydrate 钼酸铵 12054-85-2

Wako 011-03211 Hexaammonium Heptamolybdate Tetrahydrate 钼酸铵 12054-85-2

011-03211

Ammonium Molybdate Tetrahydrate

 

Guaranteed Reagent 100 g

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

Wako和光纯药 Lysyl Endopeptidase 赖氨酰内肽酶,质谱级

Wako和光纯药 Lysyl Endopeptidase 赖氨酰内肽酶,质谱级

Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)

Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase®. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.

Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C) is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.

赖氨酰内肽酶®,质谱级 (Lys-C)

蛋白质组分析中最重要的技术之一是凝胶内消化蛋白质斑点/条带,这些蛋白质斑点/条带已使用消化酶(如胰蛋白酶和赖氨酰内肽酶® )通过电泳分离。蛋白质可以通过凝胶内消化产生的肽的质谱分析来识别,并且可以获得关于翻译后修饰的进一步信息。

Lysyl Endopeptidase ® , 质谱级 (Lys-C) 是一种冷冻干燥的产品,保留了足够的凝胶内消化活性,并以非常小的数量包装以方便使用。

  • Recombinant
    Lysyl Endopeptidase®
  • Biochemistry Grade
    Lysyl Endopeptidase®
  • Features
  • Application Data
  • References
  • Product List
  • Related Product List
  • Related Information

Features

  • High specificity and efficiency of protein digestion allow for easy database searches by peptide mass.
  • Improved cleavage at lysine residue and increase in the number of peptides are obtained by combination with trypsin.
  • Packed in very small quantities according to the amounts used so that sufficient activity for in-gel digestion may be retained.

Application Data

Comparison of In-gel Digestion Using Trypsin (Tp), Lysyl Endopeptidase® (Lep) and Lep Combined with Tp (Lep +Tp)

BSA band (100ng) resolved by SDS-PAGE was in-gel digested with Tp, Lep and Lep +Tp and analyzed by MALDI-TOFMS. The figure shows the individual mass spectra. The evaluation of these peptidases is summarized in the table.

使用胰蛋白酶 (Tp)、赖氨酰内肽酶® (Lep) 和 Lep 结合 Tp (Lep +Tp)进行凝胶内消化的比较

通过 SDS-PAGE 解析的 BSA 条带 (100ng) 用 Tp、Lep 和 Lep +Tp 进行凝胶内消化,并通过 MALDI-TOFMS 进行分析。该图显示了单独的质谱图。这些肽酶的评价总结在表中。

Table: Comparison of Trypsin (Tp), Lysyl Endopeptidase® (Lep) and Lep + Tp

Tp Lep Tp + Lep
Cleavage site C terminal of Arg + Lys C terminal of Lys C terminal of Arg + Lys
Missed cleavage (Rates of missed cleavage) Many (8%) Very few (0%) Few (3%)
No. of identified peptides 17 19 22

Data provided by: Wada, Y., Osaka Women’s and Children’s Hospital

These results indicate there are very few missed cleavages obtained by Lep digestion. When Tp is used concomitantly with Lep, missed cleavages decrease and the number of identified peptides increase compared to when only Tp is used.

*The value resulted from subtracting the coverage obtained when database searches were performed with Missed cleavage 0 from that obtained when performed with Missed cleavage 1. “Coverage” is the percentage of peptides obtained after in-gel digestion in the whole sequence.

Mass spectrum: Comparison of Trypsin (Tp), Lysyl Endopeptidase® (Lep) + Tp

Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)
Data provided by: Wada, Y., Osaka Women’s and Children’s Hospital

References  参考文献

  1. Wada, Y. and Kadoya, M.: J. Mass Spectrom., 38, 117(2003).
  2. Shevchenko, A., Wilm, M., Vorm, O. and Mann., M.: Anal. Chem., 68, 850(1996).

Product List

Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)

for Proteome Research

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
125-05061
20ug x5
List Price
JPY 75,000

In stock in Japan

Distributor
121-05063
20ug
List Price
JPY 20,000

In stock in Japan

Trypsin, Mass Spectrometry Grade

Trypsin, from Porcine Pancreas, Mass Spectrometry Grade

for Proteome Research

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
GHS :
Comparison
Product Number
Package Size
Price
Inventory
Distributor
202-15951
20ug x5
List Price
JPY 17,900

In stock in Japan

Related Product List

Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)

for Cell Culture

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
124-06871
20ug
List Price
JPY 35,000

Lysyl Endopeptidase®, Biochemistry Grade (Lys-C)

Lysyl EndopeptidaseR (Lys-C)

for Biochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
129-02541
1vial(10AU)
Inquire

In stock in Japan

Distributor
125-02543
1vial(2AU)
Inquire

In stock in Japan

Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)

Lysyl Endopeptidase®, Biochemistry Grade (Lys-C)

Related Information

Category

  1. Life Science
  2. Proteomics
  3. Enzyme
  1. Life Science
  2. Enzyme
  3. Protease
  1. Cell Culture
  2. Biopharmaceuticals Manufacturing
  3. Quality Control Test

Product content may differ from the actual image due to minor specification changes etc.

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

库存信息随时变更,准确货期以下单时确认为准。
重要提醒:该中文说明为国内翻译版本,仅供参考,与官网英文版有不符之处,以英文版说明为主。

PUREfrex®1.0

PUREfrex® 1.0PUREfrex®1.0

自2022年12月起,PUREfrex® 试剂盒内的Solution Ⅰ的原材料已更换,具体详情请点击:PUREfrex® 系列试剂盒部分组成及制备方法变更通知

PUREfrex® 1.0是基于PURE system开发的无细胞蛋白合成试剂盒,由转录、翻译、能量回收所需的蛋白和核糖体组成。除活性蛋白表达以外,还可广泛用于display技术和翻译机制的研究等。

PUREfrex® 1.0不含分子伴侣。因此,根据合成蛋白不同,可能无法形成正确的高阶结构,合成的蛋白也可能不溶于水。这时,只需在蛋白合成时添加分子伴侣即可解决。GeneFrontier也推出了适用于本试剂盒的分子伴侣DnaK mix(#PF003-0.5)GroE mix(#PF004-0.5)


PUREfrex® 1.0含有DTT(反应液中的最终浓度为2mM),可在还原条件下进行蛋白合成反应。合成需要形成二硫键(SS键:ss-bond或二硫桥:disulfide bridge)的蛋白时,建议添加DsbC Set (#PF005-0.5)PDI Set (#PF006-0.5)使用。


产品编号

产品名称

产品规格

PF201-0.25

PUREfrex® 1.0

250 µL反应用

PF001-2ML 

[New]  

PUREfrex® 1.0

2 mL反应用

PF001-10ML

PUREfrex® 1.0

10 mL反应用

(5×2 mL) 

产品组成

PUREfrex®1.0中各Solution成分详情点击此处下载。

250 µL反应用


溶液

产品规格

产品成分

开封后的储存温度 *1

Solution Ⅰ

125 μL

氨基酸、NTP、tRNA、

酶底物等。

-20℃

Solution Ⅱ

12.5 μL

蛋白(30%甘油溶液)

-20℃或-80℃ *2

Solution Ⅲ

12.5 μL

核糖体(20 µM)

-80℃ *2

DHFR DNA *3

碱基序列  

10 μL

含大肠杆菌DHFR基因的 PCR 产物(20 ng/μL)

-20℃

2 mL 反应用

溶液

产品规格

产品成分

开封后的储存温度*1

Solution Ⅰ

1,000 μL

氨基酸、NTP、tRNA、

酶底物等。

-20℃

Solution Ⅱ

100 μL

蛋白(30%甘油溶液)

-20℃或-80℃ *2

Solution Ⅲ

100 μL

核糖体(20 µM)

-80℃ *2

DHFR DNA *3

碱基序列  

10 μL

含大肠杆菌DHFR基因的PCR产物(20 ng/μL)

-20℃

*1)试剂盒开封前,请在-80℃条件下储存。

*2)在-80°C条件下储存使用后的剩余反应溶液时,应在储存前使用液氮或干冰/乙醇等进行速冻。请根据需求进行分装,避免反复冻融。

*3)当用作蛋白合成的阳性对照时,请在20 µL合成反应液中添加1 µL DHFR DNA。

使用方法

 


使用PUREfrex®1.0可在任意体积的反应液下进行蛋白合成。例如,20 μL时的制备方法如下所示。

1.    将Solution Ⅰ在室温~37℃下加热1 min左右至完全融解,随后置于冰上。

2.    将Solution Ⅱ和Solution Ⅲ置于冰上融解。

3.    将融解的SolutionⅠ,Ⅱ,Ⅲ轻轻涡旋后,离心并收集试管底部的内容物。

4.    请根据以下配比制备反应液。(DNA 添加量为每kbp 0.5-3 ng/ μL)

RNase-Free Water

8-X μL

Solution Ⅰ

10 μL

Solution Ⅱ

1 μL

Solution Ⅲ

1 μL

模板DNA 注1

X μL

Total

20 μL

5.在37°C的加热块或水浴中反应 2~4 h,合成蛋白注2

6.合成的蛋白可用于多种用途注3

注意事项

注1) 模板DNA请根据目标蛋白自行制备。关于制备方法,请参阅“关于模板DNA”。

注2) 如果在气相恒温槽(如培养用恒温器)中进行反应,反应液的温度上升需花费更多的时间,合成量会有所降低

注3) 通过SDS-PAGE确认合成时,请用水稀释反应液后进行电泳。详情请点击PUREfrex反应液的SDS-PAGE查看。

使用注意事项

PUREfrex® 为研究用试剂,不可用于包含人在内的动物给药、临床和诊断等其他用途。此外,请勿用于食品或家庭用途。

使用 PUREfrex® 时,请使用不含RNase的水、试剂和设备。 此外,建议佩戴手套和口罩。