Wako 和光纯药 011-27991 Anti Iba1, Goat  Iba1抗体，山羊源多克隆抗体

名称：Anti Iba1, Goat
用途：for Immunochemistry

Storage Condition : Keep at -20 degrees C.

Application Data

Immunohistochemistry (fluorescent staining)

Immunohistochemistry of frozen section in mouse model of Alzheimer’s disease (APP^NL-G-F mouse)　brain cerebral neocortex using anti Iba1, goat polyclonal antibody at dilution of 1:1,000, and anti goat IgG, Alexa Flour 488-conjugated. Aβ were stained by 0.001% FSB solution(Amyloid fluorescent probe). The data were provided by Dr. Sakakibara, National Center for Geriatrics and Gerontology in Japan.

 

Immunohistochemistry of frozen section in rat(left) and mouse(right) brain cerebral cortex using anti Iba1, goat polyclonal antibody at dilution of 1:250, and anti goat IgG, Alexa Flour 488-conjugated.
The data were provided by Dr. Nakajima from Soka University in Japan.

Immunohistochemistry(DAB staining)

Sample: paraffin section of mouse brain frontal lobe
1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, biotin-conjugated
Antigen retrieval: 10mM citric acid buffer(pH6.0), 90℃, 10min

Western Blotting

The data were provided by Dr. Nakajima from Soka University in Japan.

Sample:
Microglia from rat primary culture 10μg
Neuron from rat primary culture 10μg
Astrocyte from rat primary culture 10μg
Cerebral cortex from rat brain 100μg

1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, HRP-conjugated

Wako 018-22381 Anti DYKDDDDK tag, Monoclonal Antibody说明书

WAKO 和光纯药代理商上海金畔生物，欢迎广大新老客户访问日本Wako试剂官网或者咨询我们获取更多详细信息。

for Immunochemistry

Storage Condition : Keep at -20 degrees C.

Host : Mouse

Clone No. : 1E6

Classification : Monoclonal

Overview / Applications

Outline	The DYKDDDDK was consisted of 9 amino acids and has been used as the tag for detection and/or purification of recombinant protein. Monoclonal anti DYKDDDDK tag antibody was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic DYKDDDDK peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites. This product is for research use only. Do not administer it to human.[Concentration] Displayed on label. [Formulation] 1 X PBS aqueous solution with 50% glycerol. [Clone No.] 1E6 [Subclass] IgG2b [Specificity] Specific for the DYKDDDDK peptide. [Working Dilution] Western blot 1:1,000 – 1:10,000 Immunoprecipitation 5 µg/20 µL 10% Protein A or Protein G beads slurry
Features	Applicable to Western Blot analysis and Immunoprecipitation
Instructions	Western Blot Analysis (1 : 1,000 – 1 : 10,000) Immunoprecipitation (5 μg/20 μL 10% Protein A or Protein G beads slurry)

Property

Appearance	Liquid
Composition	1xPBS aqueous solution with 50% glycerol.
Concentration	0.4 – 0.6 mg/mL Protein

Antibody Information

Antigen	DYKDDDDK tag
Antigen Alias	DYKDDDDK Peptide DYKDDDDK DYKDDDDK-tag DYKDDDDK Epitope Tag FLAG EPITOPE TAG Ftag Antibody DYKDDDDK FLAG FLAG Epitope FLAG peptide Flag-tag

Related Information

Similar items list

• DYKDDDDK tag

Product List

Product Name
Anti DYKDDDDK tag, Monoclonal Antibody(6F7), Rat Discontinued	for Immunochemistry
Anti DYKDDDDK tag, Monoclonal Antibody, Peroxidase Conjugated	for Immunochemistry
Anti DYKDDDDK tag Antibody Beads	for Immunochemistry
Anti DYKDDDDK tag Antibody Magnetic Beads	for Immunochemistry
DYKDDDDK Peptide	for Genetic Research
Carboxy-terminal DYKDDDDK-BAP, recombinant, Solution	for Genetic Research