L-012化学发光探针
生化学用 for Biochemistry
规格含量 : 90.0+%（HPLC）
制造商 : FUJIFILM Wako Pure Chemical Corporation（富士胶片和光）
储存条件：冷藏（干冰运输）
CAS RN® : 143556-24-5
分子式 : C13H8ClN4NaO2
分子量 : 310.67
◆基本信息
L-012 是一种高灵敏度的化学发光（CHL）探针，比鲁米诺（Luminol）试剂更有效。L-012 与多种类型的活性氧反应，这些活性氧来自人血液和口腔中激活的中性粒细胞或者大鼠腹膜腔。该产品可以用于有辣根过氧化物酶的ELISA 试剂盒，可提高反应灵敏度。
结构式
溶解及保存：蒸馏水溶解成20 mM/L（6.23 mg/mL）的水溶液，再用50 mM/L的Tris盐酸缓冲液（pH 7.5）稀释 至最终浓度为500 μM/L（有细胞的反应体系）。另外，在没细胞参与的反应体系中时，建议使用终浓度为5~50 μM/L。母液冷冻保存，使用时再稀释。
◆物性信息
外观
白色~黄色，结晶~粉末
溶解性
可溶于水。
Q： L-012能渗入细胞吗?
A： 由于L-012对脂质高度亲和，它比luminol和lucigenin更能具有细胞渗透性。另一方面，ROS无限制地在胞外释放。因此L-012被认为也能
检测胞外ROS。
Q： L-012的使用浓度如何?
A： 这取决于应用和条件。
[体外] 培养细胞（EoL-1细胞）： 500 ~ 800 μmol/L.
全血和分离的中性粒细胞： 400 μmol/L
多形核中性粒细胞（PMNs）（由小鼠中分离）： 2 mmol/L
无细胞的样品： 4 μmol/L, 15 μmol/L, and 50 μmol/L
[体内] Balb/c 小鼠[皮下]： 20 ~ 80 μL of L-012(5mg/mL)
Balb/c 小鼠[腹膜内]： 100 μL of L-012 (15mg/mL)
C57BL/10.Q (B10.Q) 小鼠 [腹膜内]： 1 ~ 75 mg/kg
Q： 是否可用于动物活体成像？
A： 如果所用的小动物成像仪中有发光检测探头，就可以进行检测。以下是文献报道过的仪器型号：
・IVIS 100 imaging systems
・Infinite M200 [Tecan]
・Luminescence Reader BLR-201 [Aloka]
・Beckman-Coulter DTX880 multimode plate reader [Beckman]
Q： 是否可用于固定细胞？
A： 目前尚无证据表明，活性氧（ROS）在细胞活性停止后仍然存在。普遍看法是认为在细胞活性停止后（细胞死亡）ROS消失。固定组织或
细胞会停止细胞活性，因此不建议用于ROS检测。
本品无需固定检测对象即可检测到ROS，使用方法包括但不限于将其添加到细胞培养上清液中或进行动物腹腔注射。
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