Harlan Teklad NAFLD and NASH

Harlan Teklad NAFLD and NASH

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Teklad diet, bedding and enrichment    Teklad laboratory animal diets   Custom research diets   NAFLD and NASH

诱导啮齿动物NAFLD/NASH的饮食方法可分为两大类:

  • 长期喂饲可诱发肥胖、代谢综合症及轻微纳什或
  • 短时间喂饲可诱发严重纳什的肝脏特征而不引起肥胖或胰岛素抵抗的饮食。

下表突出了上述两个类别的饮食选择。有关NAFLD/NASH模型的更完整描述,请参见表后面的下拉菜单。

Dietary methods to induce NAFLD/NASH in rodents can be split into two common categories:

  • diets fed for longer periods of time to induce obesity, metabolic syndrome, and mild NASH or
  • diets fed for short periods of time to induce hepatic features of severe NASH without inducing obesity or insulin resistance

This page provides further information on dietary methods to induce NAFLD/NASH. We’ve also prepared a downloadable NASH/NAFLD mini paper.

The tables below highlight diet options from both of the above categories. For more complete descriptions of NAFLD/NASH models see the drop down menus that follow the tables.

Diet options for inducing obesity, metabolic syndrome and mild NAFLD/NASH
Diet features Western/Fast Food ALIOS FPC diet
Product Code TD.88137 TD.06303 TD.160785 PWD dough

TD.190142 pellet

Fat, % Kcal 42 45 52
Fat Sources,
% by weight
21% milk fat 22% hydrogenated vegetable oil
1% soybean oil
19% hydrogenated vegetable oil
6% milk fat
4% palmitic acid
Fatty acid profile,
% total fat
66% saturated
30% monounsaturated
4% polyunsaturated
23% saturated
31% monounsaturated (cis)
12% polyunsaturated (cis)
34% trans
43% saturated
27% monounsaturated (cis)
7% polyunsaturated (cis)
23% trans
Sugars, % by weight 34.5% sucrose 22.4% sucrose 34.5% sucrose
Cholesterol, % by weight 0.2 0 1.25
Modifications TD.96121 1.25% cholesterol
TD.120528 Increased sucrose, 1.25% cholesterol
TD.120330 0.2% cholesterol
TD.130885 0.2% cholesterol, 27% sucrose
TD.140154 adds customer supplied palmitic acid

For high fat diet options to induce uncomplicated NAFLD see our Diet Induced Obesity page.

Diet options for inducing more severe hepatic NAFLD/NASH without obesity or metabolic syndrome
Diet features High Fat, Cholesterol & Cholate Methionine/choline deficient (MCD)
Product Code TD.02028 TD.90262
Fat, % Kcal 42 22
Fat Sources,
% by weight
21% milk fat 10% corn oil
Fatty acid profile,
% total fat
66% saturated
30% monounsaturated
4% polyunsaturated
14% saturated
28% monounsaturated
58% polyunsaturated
Sugars, % by weight 33.3% sucrose 46% sucrose
Cholesterol, % by weight 1.25 0
Cholate Source, % by weight 0.5 0
Related diets TD.09237 15% milk fat, 1% cholesterol
TD.88051 Hybrid version
TD.94149 MCD control diet

Diets inducing obesity, metabolic syndrome and mild NAFLD/NASH

Western or fast food style diets fed to induce NASH with metabolic syndrome contain 40 – 45% kcal from milkfat (a fat source high in palmitate) with added cholesterol (0.15 – 2%) and are high in sucrose (>30%). Dietary palmitate and cholesterol have both previously been associated with the progression from simple steatosis to NASH.

Examples:

  • TD.88137       Adjusted Calories Diet (42% from fat)
  • TD.96121       21% MF, 1.25% Chol. Diet
  • TD.120528     42% Kcal/Fat Diet (Incr. Sucrose, 1.25% Chol.)

Research use:

These diets can induce obesity, metabolic syndrome, and simple steatosis within nine weeks of feeding. Increased hepatic inflammation has been observed after 12 weeks of feeding. NASH typically requires longer feeding with fibrosis developing within nine months and late stage fibrosis including hepatic ballooning occurring after 14 – 20 months of feeding. Increasing dietary sucrose (~41%) and cholesterol (~1.25%) accelerates the NASH phenotype with steatosis, inflammation and hepatocyte ballooning observed within 12 weeks. In addition to feeding a high fat diet, providing a glucose/fructose mixture in the drinking water may further promote NASH development.

Select References:

Charlton, M., et al., Fast food diet mouse: novel small animal model of NASH with ballooning, progressive fibrosis, and high physiological fidelity to the human condition. Am J Physiol Gastrointest Liver Physiol, 2011. 301(5): p. G825-34. http://www.ncbi.nlm.nih.gov/pubmed/21836057

Gores, G., Charlton M, Krishnan A, Viker K, Sanderson S, Cazanave S, McConico A, Masuoko H. Am J Physiol Gastrointest Liver Physiol, 2015. 308: p. G159. http://ajpgi.physiology.org/content/308/2/G159

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. http://www.ncbi.nlm.nih.gov/pubmed/19119140

Ioannou, G.N., et al., Hepatic cholesterol crystals and crown-like structures distinguish NASH from simple steatosis. J Lipid Res, 2009. 54(5): p. 1326-34. http://www.ncbi.nlm.nih.gov/pubmed/23417738

Alkhouri, N., et al., Adipocyte apoptosis, a link between obesity, insulin resistance, and hepatic steatosis. J Biol Chem, 2010. 285(5): p. 3428-38. http://www.ncbi.nlm.nih.gov/pubmed/19940134

Dixon, L.J., et al., Caspase-1 as a central regulator of high fat diet-induced non-alcoholic steatohepatitis. PLoS One, 2013. 8(2): p. e56100. http://www.ncbi.nlm.nih.gov/pubmed/23409132

DeLeve, L.D., et al., Prevention of hepatic fibrosis in a murine model of metabolic syndrome with nonalcoholic steatohepatitis. Am J Pathol, 2008. 173(4): p. 993-1001. http://www.ncbi.nlm.nih.gov/pubmed/18772330

VanSaun, M.N., et al., High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol, 2009. 175(1): p. 355-64. http://www.ncbi.nlm.nih.gov/pubmed/19541928

Asgharpour, A., et al., A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer. J Hepatol, 2016. 65(3): p. 579-88. http://www.ncbi.nlm.nih.gov/pubmed/27261415

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. http://www.ncbi.nlm.nih.gov/pubmed/18772365

Tsuchida, T., et al., A simple diet-and chemical-induced murine NASH model with rapid progression of steatohepatitis, fibrosis and liver cancer. Journal of hepatology, 2018. 69(2):385-395. https://www.ncbi.nlm.nih.gov/pubmed/29572095

The American Lifestyle-Induced Obesity Syndrome (ALIOS) model involves feeding the “American fast food” diet high in trans-fats and sugar. Dietary trans-fats from hydrogenated vegetable shortening (HVO) are associated with increased insulin resistance and hepatic inflammation in rodent NASH models. In addition to diet, a glucose/fructose solution is added to the drinking water and sedentary behavior promoted by removing the overhead cage feeders in this model.

Examples:

  • TD.06303       22% HVO Diet
  • TD.120330     22% HVO + 0.2% Cholesterol Diet
  • TD.130885     ALIOS with Added Sugar

Research use:

The ALIOS model develops obesity with insulin resistance, elevated ALT levels, and steatosis within 16 weeks. Increased inflammation and early development of fibrosis have been observed at 6 months. Severe steatosis with fibrosis and inflammation develops within 12 months of feeding with 50% of the mice reportedly developing hepatic neoplasms. Adding cholesterol (0.2%) to the American Fast Food diet may accelerate NASH phenotype development.

Select References:

Koppe, S.W., et al., Trans fat feeding results in higher serum alanine aminotransferase and increased insulin resistance compared with a standard murine high-fat diet. Am J Physiol Gastrointest Liver Physiol, 2009. 297(2): p. G378-84. http://www.ncbi.nlm.nih.gov/pubmed/19541924

Tetri, L.H., et al., Severe NAFLD with hepatic necroinflammatory changes in mice fed trans fats and a high-fructose corn syrup equivalent. Am J Physiol Gastrointest Liver Physiol, 2008. 295(5): p. G987-95. http://www.ncbi.nlm.nih.gov/pubmed/18772365

Mells, J.E., et al., Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol, 2012. 302(2): p. G225-35. http://www.ncbi.nlm.nih.gov/pubmed/22038829

Dowman, J.K, et al., Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle. Am J Pathol, 2014. 184(5):1550-1561. https://www.ncbi.nlm.nih.gov/pubmed/24650559 

Mells, J.E., et al., Saturated fat and cholesterol are critical to inducing murine metabolic syndrome with robust nonalcoholic steatohepatitis. J Nutr Biochem, 2014. 26(3): p. 285-92. http://www.ncbi.nlm.nih.gov/pubmed/25577467

The Fructose, Palmitate, Cholesterol and Trans-Fat (FPC) diet is a recent NASH diet that includes Western and ALIOS model diets to achieve both metabolic and hepatic NASH features within an accelerated time frame. Key features of the FPC diet include 1) a lower Met content than typical rodent diets by decreasing total protein without supplementing sulfur amino acids; 2) choline supplementation is lower than typical but is not considered deficient; 3) high in sucrose (~34% by weight); 4) 1.25% cholesterol; 5) 52% kcal from fat with fat sources including milkfat fat, palmitic acid and hydrogenated vegetable shortening to provide trans-fats. Like the ALIOS model, the FPC model also provides a glucose/fructose solution to the drinking water.

Examples:

  • TD.160785     52 kcal/Fat Diet (C16:0, HVO, AMF, Choline/Met)

Research use:

Male C57BL/6J mice fed the FPC diet and provided a glucose/fructose drinking solution developed insulin resistance and NAFLD with inflammation, hepatocyte death, and fibrosis within 16 weeks.

Select References:

Wang, X., et al., Hepatocyte TAZ/WWTR1 promotes inflammation and fibrosis in nonalcoholic steatohepatitis. Cell Metab, 2016. 24(6): p. 848-62. https://www.ncbi.nlm.nih.gov/pubmed/28068223  

Zhu, C., et al., Hepatocyte Notch activation induces liver fibrosis in nonalcoholic steatohepatitis. Sci Transl Med, 2018. 10(468). https://www.ncbi.nlm.nih.gov/pubmed/30463916

Common diets to induce obesity (DIO) can be fed to induce uncomplicated NAFLD. These high fat diets typically contain 40–60% kcal from fat without supplemented cholesterol or cholate. Simple sugars such as sucrose or fructose can also be supplemented via diet or water to progress the fatty liver phenotype. Diets can be in pellet or powder/dough form depending on the formula. Some models require limited physical activity and in those cases diets can be fed inside the cage. For more information see our Diet Induced Obesity page.

Examples:

  • TD.08811       45%kcal Fat Diet (21% MF, 2% SBO)
  • TD.06414       Adjusted Calories Diet (60/Fat)

Research use:

In susceptible rodent models, high fat diets are commonly used to induce NAFLD with obesity and insulin resistance common metabolic features associated with NASH in humans. However, the degree of NASH pathology (steatosis, inflammation, and fibrosis) is limited or mild and varies depending on the animal model, length of feeding, and dietary components.

Diets to induce severe hepatic NAFLD/NASH without obesity or metabolic

COLLAPSE ALL TABS

Originally formulated to induce mild atherosclerosis in wild-type rodents, high fat diets containing added cholesterol (1 – 1.25%) and cholate (0.5% as sodium cholate or cholic acid) have also been useful in inducing NASH. This diet option includes purified “Western” style diets with increased cholesterol and cholate and also hybrid diets. Hybrid diets were originally developed by Beverly Paigen and colleagues by mixing a natural ingredient mouse diet in a 3:1 ratio with a concentrated purified diet (containing 5% cholesterol and 2% sodium cholate) resulting in a diet containing ~15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. Although a less refined approach, the hybrid diet is associated with increased gallstone formation and liver damage as compared to similar purified diets.

Examples:

  • TD.02028       Atherogenic Rodent Diet
  • TD.88051       Cocoa Butter Diet and Purina Mouse Chow
  • TD.09237       15% AMF Diet (1% Chol, 0.5% NaChol)

Research use:

Atherogenic diets are able to induce varied degrees of NASH with increased hepatic inflammation with early fibrosis observed after ten weeks of feeding. However, the metabolic profile typical in human NASH (obesity with insulin resistance) is not recapitulated in this model with animals typically maintaining similar body weights as control fed groups without the development of metabolic syndrome.

Select References:

Nishina, P.M., J. Verstuyft, and B. Paigen, Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res, 1990. 31(5): p. 859-69. http://www.ncbi.nlm.nih.gov/pubmed/2380634

Kamari, Y., et al., Lack of interleukin-1alpha or interleukin-1beta inhibits transformation of steatosis to steatohepatitis and liver fibrosis in hypercholesterolemic mice. J Hepatol, 2011. 55(5): p. 1086-94. http://www.ncbi.nlm.nih.gov/pubmed/21354232

Kim, D.G., et al., Non-alcoholic fatty liver disease induces signs of Alzheimer’s disease (AD) in wild-type mice and accelerates pathological signs of AD in an AD model. J Neuroinflammation, 2016. 13: p. 1. http://www.ncbi.nlm.nih.gov/pubmed/26728181

Madrigal-Perez, V.M., et al., Preclinical analysis of nonsteroidal anti-inflammatory drug usefulness for the simultaneous prevention of steatohepatitis, atherosclerosis and hyperlipidemia. Int J Clin Exp Med, 2015. 8(12): p. 22477-83. http://www.ncbi.nlm.nih.gov/pubmed/26885230

Savransky, V., et al., Chronic intermittent hypoxia causes hepatitis in a mouse model of diet-induced fatty liver. Am J Physiol Gastrointest Liver Physiol, 2007. 293(4): p. G871-7. http://www.ncbi.nlm.nih.gov/pubmed/17690174

Methionine and choline deficient (MCD) diets are amino acid defined rodent diets deficient in methionine and choline, high in sucrose (>40% by weight) with ~10% corn oil by weight. Methionine and choline deficiency decreases fat oxidation and export of fat from the liver. Dietary sucrose is necessary for hepatic lipid accumulation and oxidation. The polyunsaturated fat in corn oil promotes hepatic lipid oxidation.

Example:

  • TD.90262       Methionine/Choline Deficient Diet

Control:

  • TD.94149       Amino Acid Control Diet

Research use:

Steatosis, increased serum alanine aminotransferase (ALT), inflammation, and hepatic fat oxidation has been observed within three weeks of feeding the MCD diet with fibrosis development after six weeks. This dietary model does not produce metabolic syndrome (an aspect of NASH in human models) and progressive weight loss (up to 40%) is associated with the MCD diet feeding.

蛋氨酸/胆碱缺乏症(MCD)日粮
蛋氨酸和胆碱缺乏(MCD)饲料是一种氨基酸定义的啮齿动物饲料,缺乏蛋氨酸和胆碱,蔗糖含量高(体重>40%),玉米油含量约10%。蛋氨酸和胆碱缺乏减少脂肪氧化和从肝脏输出脂肪。日粮蔗糖对肝脏脂质的积累和氧化是必需的。玉米油中的多不饱和脂肪促进肝脏脂质氧化。

例子:TD.90262 蛋氨酸/胆碱缺乏症饮食
管制:TD.94149 氨基酸控制饮食

研究用途:

观察到脂肪变性、血清丙氨酸转氨酶(ALT)升高、炎症和肝脂肪氧化反应。这种饮食模式不会产生代谢综合症(在人类模型中是NASH的一个方面)和累进性减肥(高达40%)与MCD饮食喂养有关。

Select References:

Pickens, M.K., et al., Dietary sucrose is essential to the development of liver injury in the MCD model of steatohepatitis. J Lipid Res, 2009. 50(10):2072-82.  http://www.ncbi.nlm.nih.gov/pubmed/19295183

Li, Z.Z., et al., Hepatic lipid partitioning and liver damage in nonalcoholic fatty liver disease: role of stearoyl-CoA desaturase. J Biol Chem, 2009. 284(9): p. 5637-44. http://www.ncbi.nlm.nih.gov/pubmed/19119140

Lee, G.S., et al., Polyunsaturated fat in the methionine-choline-deficient diet influences hepatic inflammation but not hepatocellular injury. J Lipid Res, 2007. 48(8): p. 1885-96. http://www.ncbi.nlm.nih.gov/pubmed/17526933

Vetelainen, R., A. van Vliet, and T.M. van Gulik, Essential pathogenic and metabolic differences in steatosis induced by choline or methione-choline deficient diets in a rat model. J Gastroenterol Hepatol, 2007. 22(9): p. 1526-33. http://www.ncbi.nlm.nih.gov/pubmed/17716355

Leclercq, I.A., et al., Intrahepatic insulin resistance in a murine model of steatohepatitis: effect of PPARgamma agonist pioglitazone. Lab Invest, 2007. 87(1): p. 56-65. http://www.ncbi.nlm.nih.gov/pubmed/17075577

Kashireddy, P.R. and M.S. Rao, Sex differences in choline-deficient diet-induced steatohepatitis in mice. Exp Biol Med (Maywood), 2004. 229(2): p. 158-62. http://www.ncbi.nlm.nih.gov/pubmed/14734794

Dixon, L.J., et al., Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis. Lab Invest, 2012. 92(5): p. 713-23. http://www.ncbi.nlm.nih.gov/pubmed/22411067

Dietary models of NAFLD/NASH continue to evolve with the goal of more accurately recapitulating both the metabolic and hepatic symptoms of human disease. Commonly researchers are studying the synergistic effects of various NASH dietary features to accelerate progression of the model and severity of liver disease.

A Teklad nutritionist can work with you to formulate new diets in order to investigate novel dietary models of NAFLD/NASH.

The choice of control diet is dependent on the specific research goal. Many researchers choose to compare their NAFLD/NASH diet-fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diet or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens).

Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component. In some cases, such as those studies feeding amino acid defined diets like the MCD model, a matched control diet is recommended given the very different formulations and protein sources of grain-based diets.

When making inferences about specific nutrients within the diet an ingredient matched, low fat control diet may be necessary. There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined and digestible) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible).

A very basic purified control diet would be AIN-93M TD.94048 or AIN-93G TD.94045. AIN-93 diets have a moderate amount of sucrose at ~10% with fat from soybean oil providing a healthy fatty acid profile.

Contact a nutritionist for an additional information and control diet recommendations.

对照日粮
控制饮食的选择取决于具体的研究目标。许多研究人员选择比较他们的NAFLD/纳什饮食喂养的动物和喂养一种天然成分的动物,谷物为基础的饮食(也称为标准饮食或周食)。这些饮食在营养来源和水平以及非营养因素(如植酸盐或植物雌激素)存在的情况下存在差异。

根据您的主要比较,它可能适合作为您的对照/参考组谷物基础的饮食。然而,这样的比较限制了对饮食模式和特定饮食成分的推断。在某些情况下,如那些研究喂食氨基酸定义的饮食,如mcd模型,建议一个匹配的对照饮食,考虑到非常不同的配方和蛋白质来源的谷物为基础的饮食。

当对饮食中的特定营养成分做出相应的推断时,低脂控制饮食可能是必要的。除了不同类型的碳水化合物外,还有许多不同水平和类型的脂肪,从蔗糖(高精制和可消化)到玉米淀粉(精制,但更复杂),再到抗性淀粉(精制,但不能完全消化)。

一种非常基本的纯正控制饮食将是-9300万。TD.94048或者是93g TD.94045。AIN-93日粮中含有适量的蔗糖~10%,大豆油中的脂肪提供了健康的脂肪酸谱。

Need more information? A Teklad nutritionist will work with you to determine if existing diets will meet your needs or formulate new diets to help you investigate novel dietary models of NAFLD/NASH. Contact us for a diet consultation.

Harlan TD.07864 1% Choline Diet (2018)饲料说明书

Harlan TD.07864 1% Choline Diet (2018)饲料说明书

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 988.2
Choline Chloride, customer supplied 11.8

Footnote

The 2018 Teklad Global diet with 1% added choline from choline chloride (approx. 85% choline).

Selected Nutrient Information1

  % by weight % kcal from
Protein 18.0 22.9
Carbohydrate2 47.4 60.5
Fat 5.8 16.6
Kcal/g 3.1  
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Key Features

  • Standard Diet Base
  • Choline
  • Customer Supplied Ingredient
  • Teklad 2018

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Harlan代理 TD.80396 Iron Deficient Diet 缺铁饲料说明书

Harlan代理 TD.80396 Iron Deficient Diet 缺铁饲料说明书
Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet

Harlan TD.80396  Iron Deficient Diet

Formula g/Kg
Casein, low Cu & Fe 200
DL-Methionine 3
Sucrose 549.99
Corn Starch 150
Corn Oil 50
Mineral Mix, Fe Deficient (81062) 35
Vitamin Mix, AIN-76A (40077) 10
Choline Bitartrate 2
Ethoxyquin, antioxidant 0.01

Footnote

The diet contains approximately 2-6 ppm Fe. To limit background iron, cellulose is omitted, and reagent grade, pretested calcium phosphate is used in the mineral mix. TD.80394 is a possible ingredient matched control with ~48 ppm added iron from ferric citrate. For questions about this or other iron adjusted diets

Selected Nutrient Information1

% by weight % kcal from
Protein 17.7 17.8
Carbohydrate 69.9 70.4
Fat 5.2 11.8
Kcal/g 4
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • AIN-76A Modification
  • Iron Deficient

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation not advised
    • Contact a nutritionist for recommendations

Harlan代理 TD.85419 Zinc Deficient Diet 锌缺乏饲料

Harlan代理 TD.85419 Zinc Deficient Diet 锌缺乏饲料
Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet

Harlan TD.85419  Zinc Deficient Diet

Formula g/Kg
Egg White Solids, spray-dried 200
Dextrose, monohydrate 634.2658
Corn Oil 100
Cellulose 30
Vitamin Mix, Teklad (40060) 10
Biotin 0.004
Mineral Mix, Zn Deficient (81264) 25.6902
Chromium Potassium Sulfate, dodecahydrate 0.02
Ethoxyquin, antioxidant 0.02

Footnote

Egg white solids are used instead of casein to reduce background zinc. The zinc content of the diet ranges from approximately 0.5 – 1.5 ppm. TD.85420 is a possible ingredient matched control with ~50ppm zinc from zinc carbonate. For questions about this or other zinc adjusted diets

Selected Nutrient Information

% by weight % kcal from
Protein 16.1 16.6
Carbohydrate 58.3 60.2
Fat 10 23.2
Kcal/g 3.9
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Zinc
  • Egg White Solids
  • Rodent

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Harlan TD.160118 High Fat Atherogenic Western Diet with 1% Added Choline

Harlan TD.160118 High Fat Atherogenic Western Diet with 1% Added Choline

高脂肪摄食性西方饮食,添加1%胆碱

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Harlan Teklad Custom Diet

Formula g/Kg
Casein 195
DL-Methionine 3
Sucrose 341.46
Corn Starch 138.25
Anhydrous Milkfat 210
Cholesterol 1.5
Cellulose 50
Mineral Mix, AIN-76 (170915) 35
Calcium Carbonate 4
Vitamin Mix, Teklad (40060) 10
Ethoxyquin, antioxidant 0.04
Choline Chloride 11.75

Footnote

Modification of the popular Western diet to add 1% choline from choline chloride.

Selected Nutrient Information

% by weight % kcal from
Protein 17.3 15.4
Carbohydrate 47.4 42.2
Fat 21.2 42.4
Kcal/g 4.5
  • Values are calculated from ingredient analysis or manufacturer data

Key Features

  • Purified Diet
  • Western Diet
  • Atherogenic & Obesogenic
  • Added Choline

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (crumbly)
  • Minimum order 3 Kg
  • Irradiation available upon request

Options (fees will apply)

  • Rush order (pending availability)
  • Irradiation (see Product Specific Information)
  • Vacuum packaging (1 and 2 Kg)

Harlan TD.00158 维生素A缺乏饮食 Vitamin A Deficient Diet

Harlan TD.00158 维生素A缺乏饮食 Vitamin A Deficient Diet

货号:TD.00158

英文名称:Vitamin A Deficient Diet

Formula g/Kg
Casein,”iamin-Freet”Test 200
L-Cystine 3
Com Starch 397.486
Maltodextrin 132
Sucrose 109.755
Soybean Oil 70
Cellulose 50
Mineral Mix, AIN-93G-MX (94046) 35
Choline Bitartrate 2.5
TBHQ, antioxidant 0.014
Niacin 0.03
Calcium Pantothenate 0.016
Pyridoxine HCI 0.007
Thiamin (81%) 0.006
Riboflavin 0.006
Folic Acid 0.002
Biotin 0.0002
Vitamin B.2 (0.1% in mannitol) 0.025
Vitamin E DL-alpha tocooheryl acetate (500 IU/g) 0.15
Vitamin D3 cholecalciferol (500,000 lU/g) 0.002
Vitamin K1. phyloquinone 0.0008

Footnote
This modification of diet AIN-93G (TD 94045) omits vitamin A palmitate and
substitutes alcohol-extracted casein for regular casein. Diet should contain
less than 20 IU vitamin AVkg diet.

Selected Nutrient Information

% by weight % kcal from
Protein 18.3 19.4
Carbohydrate 60.1 63.8
Fat 7 16.7
Kcalg 3.8

Key Features
+ Purified Diet
+ Vitamin A
+ VFT Casein
+ AIN-93G Modification

Key Planning Information
+ Products are made fresh to order
+ Store product at 4°C or lower   将产品存放在4°C或更低的温度下

+Use within 6 months (applicable to most diets)
+ Box labeled with product name,
manufacturing date, and lot number
+ Replace diet at minimum once per week
More frequent replacement may be advised

+ Lead time:
2 weeks non-irradiated
4 weeks irradiated

Product Specific Information
1/2”Peilet of Powder (free flowing)  1/2”粉饼(自由流动)
+ Minimum order 3 Kg
+ Irradiation not advisea
Contact a nutntionist for recommendations

Harlan Teklad 动物饲料 Vitamin A Diet(25,000)说明书

Harlan Teklad 动物饲料 Vitamin A Diet(25,000)说明书

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Formula g/Kg
Casein, “Vitamin-Free”Test 200
L-Cystine 3
Com Starch 397.486
Maltodextrin 132
Sucrose 109.705
Soybean Oil 70
Cellulose 50
Mineral Mix, AIN-93G-MX (94046) 35
Choline Bitartrate 2.5
TBHQ, antioxidant 0.014
Niacin 0.03
Calcium Pantothenate 0.016
Pyridoxine HCI 0.007
Thiamin (81%) 0.006
Riboflavin 0.006
Folic Acid 0.002
Biotin 0.0002
Vitamin B12 (0.1% in manitol) 0.025
Vitamin E DL alpha tocopheryl acetate (500 IU/g) 0.15
Vitamin D3. cholecalciferol (500,000 IU/g) 0.002
Vitamin K1, phylloquinone 0.0008
Vitamin A Acetate (500,000 IU/g) 0.05

Foothote

This modification of diet AIN-93G (TD.94045) replaces vitamin A palmitate with
tamin A acetate and increases the total concentration to approximately 25,000
/kg. Alcohol-extracted casein is substituted for regular casein.

Selected Nutrient Information

% by weight % kcal from
Protein 18.3 19.4
Carbohydrate 60.1 63.8
Fat 7 16.7
Kcal/g 3.8

Values are calculated from ingredient analysis or manufacturer data

Key Features

+ Purifield   Diet
+Vitamin A
+AlN 93G Modification
+ Rodent

Key Planning Information

+ Product are made Fresh fo order
+ store product at 4° C or IoWer
+ Use within 6 months(appIicable to most diets)
+ Box Labeled with product name
manufaGturing date,and Iot number
+ Replace diet at nninimunη once per weekation

Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 使用说明

Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 使用说明

Harlan Teklad动物饲料
Harlan代理–上海金畔生物科技有限公司
欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

啮齿动物饲料(2018,625强力霉素)

Formula g/Kg
2018, Teklad Global 18% Protein Rodent Diet 999.375
Doxycycline Hyclate 0.625

Footnote   Harlan动物饲料 TD.01306 Rodent Diet (2018, 625 Doxycycline) 使用说明

This diet was designed to deliver a daily dose of 2-3 mg of doxycycline based on consumption of 4-5 g/d by a mouse. Doxycycline hyclate contains approximately 87% doxycycline.

这种饮食被设计为以小鼠每天4-5 g / d的消耗量提供2-3 mg强力霉素的日剂量。 盐酸多西环素含有约87%的多西环素。

Selected Nutrient Information

% by weight % kcal from
Protein 18.2 22.9
Carbohydrate2 48.0 60.5
Fat 5.8 16.6
Kcal/g 3.2
  • Values are calculated from ingredient analysis or manufacturer data
  • Estimated digestible carbohydrate

Key Features

  • Standard Diet Base
  • Doxycycline
  • Gene Induction
  • 2018

Key Planning Information

  • Products are made fresh to order
  • Store product at 4°C or lower
  • Use within 6 months (applicable to most diets)
  • Box labeled with product name, manufacturing date, and lot number
  • Replace diet at minimum once per week

More frequent replacement may be advised

  • Lead time:
  • 2 weeks non-irradiated
  • 4 weeks irradiated

Product Specific Information

  • 1/2″ Pellet or Powder (free flowing)
  • Minimum order 3 Kg
  • Irradiation available upon request

Harlan动物饲料 AIN diet formulas

Harlan动物饲料 AIN diet formulas

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

These formulas are purified diets with commonly used refined ingredients. These basic formulas are often modified for a specific research purpose, such as altering the fat source, a vitamin or mineral level, or adding a compound. See the FAQ section to learn more about the history of these formulas, AIN-76A, AIN-93G, and AIN-93M.

  • CA.170481 AIN-76A purified diet
  • TD.94045 AIN-93G purified diet
  • TD.94048 AIN-93M purified diet

These formulas (as well as other purified diets) do not contain alfalfa and can be used to reduce background autofluorescence in certain imaging applications. Our Global Rodent Diets are also alfalfa-free and are suitable for imaging work. Please contact us for further information about these formulas or modifications.

Other related product codes:

  • TD.94096 version of AIN-76A suitable for irradiation (vitamin levels are increased)
  • TD.97184 version of AIN-93G suitable for irradiation (vitamin levels are increased)
  • TD.00102 version of AIN-93M suitable for irradiation (vitamin levels are increased)
  • TD.95092 modification of AIN-93G where soybean oil is replaced with corn oil.

Harlan AIN diet formulas

这些配方是含有常用精制成分的纯正饮食。这些基本配方通常是为特定的研究目的而修改的,例如改变脂肪来源、维生素或矿物质水平,或添加一种化合物。见常见问题了解更多关于这些公式的历史的章节,AIN-76A,AIN-93g,和AIN-93m。

  • CA.170481AIN-76A纯饲料
  • TD.94045AIN-93g纯饲料
  • TD.9404893米纯饲料

这些配方(以及其他纯化饲料)不含苜蓿,可用于减少背景自荧光在某些成像应用中。我们的全球啮齿动物饮食也是无苜蓿的,适合成像工作.有关这些公式或修改的进一步信息,请与我们联系。

其他相关产品代码:

  • TD.94096适合辐照的AIN-76A版本(维生素含量增加)
  • TD.97184适合辐照的an-93g版本(维生素含量增加)
  • TD.00102AIN-93m适合辐照的版本(维生素含量增加)
  • TD.95092用玉米油代替大豆油的n-93g改性。

Harlan Teklad Diet ingredients

Harlan Teklad Diet ingredients

Harlan Teklad动物饲料

Harlan代理

欢迎新老客户访问Harlan官网或者咨询中国代理商上海金畔生物获取更多详细资料。

Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets + Diet ingredients

You may want to prepare your own diet at your research site or facility, and if that is the case, you depend on the ingredients being of the highest quality. Envigo can provide you with many of the same ingredients used to produce our advanced Teklad custom research diets so you can develop your own formula. And as always, our nutritional experts are here to help you make the best decisions for your ingredients and ultimate formulation.

Our ingredients

We offer several ingredients for individual sale so that you can develop your own blends.

“VFT” casein

  • “Vitamin-free” test casein (alcohol-extracted) is best suited for purified test diet formulas where fat or vitamin content needs to be precisely controlled
  • The reduced levels of many vitamins in VFT casein make it the preferred protein source for many vitamin studies. Thus, the nickname “Vitamin-free” was given to the extracted casein many years ago

Vitamin mixes

  • The common vitamin mixes shown below use sucrose or corn starch as a carrier
  • Customized vitamin mixes may use cellulose if a non-nutritive carrier is necessary
  • Vitamin mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of your laboratory animal
  • For those preparing their own diets, vitamin premixes are an efficient way to add vitamins to a diet
  • Vitamin mixes should be stored at 4 degrees celsius or lower upon receipt. Storage at –20 degrees celsius is recommended if the mix will be used beyond 1 month
  • Minimum order is 500 g for both stock and custom vitamin mixes
  • Our available vitamin mixes are commonly used in diets, and are available from stock
  • Vitamin mixes are shipped within a few days of order

Our Formula examples

  • CA.40060 Teklad vitamin mix
  • CA.40077 AIN-76A vitamin mix
  • TD.94047 AIN-93 vitamin mix

Mineral mixes

  • The common mineral mixes shown below as examples use sucrose as a carrier
  • Customized mineral mixes could use cellulose or no carrier if a non-nutritive carrier is necessary
  • Our mineral mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of the animal
  • For those preparing their own diets, mineral premixes are an efficient way to add minerals to a diet
  • Mineral mixes should be stored in a dry cool environment and used within a year of receipt. The minimum order is 500 g for both stock and custom mineral mixes
  • These mineral mixes are commonly used in diets, and are available from stock
  • Our mineral mixes are shipped within a few days of order

Formula examples:

  • CA.170915 AIN-76 mineral mix
  • TD.94046 AIN-93G mineral mix
  • TD.94049 AIN-93M mineral mix

Other mixes (may or may not be available from stock):

  • TD.83171 Vitamin mix without A, D, E, choline
  • TD.81062 Iron deficient mineral mix based on AIN-76A
  • TD.79055 Calcium and phosphorus deficient mineral mixed based on AIN-76
  • TD.98057 Calcium and phosphorus deficient mineral mixed based on AIN-93
  • CA.170760 Rogers-Harper mineral mix

你可能想要在你的研究地点或设施准备你自己的饮食,如果是这样的话,你取决于成分是最高的质量。Envigo可以为您提供许多相同的成分,用于生产我们先进的特克拉德定制研究饮食,以便您可以开发自己的配方。和往常一样,我们的营养专家在这里帮助您为您的配料和最终配方做出最佳决定。

我们的配料

我们提供几个配料供个人销售,以便您可以开发自己的混合。

“VFT”酪蛋白

  • “无维生素”测试酪蛋白(酒精提取)最适合于那些需要精确控制脂肪或维生素含量的纯正试验饮食配方。
  • VFT酪蛋白中许多维生素含量的降低使得它成为许多维生素研究的首选蛋白质来源。因此,“无维生素”的绰号是在许多年前给提取出来的酪蛋白起的。

维生素混合物

  • 以下常见的维生素混合物使用蔗糖或玉米淀粉作为载体。
  • 定制的维生素混合物可能使用纤维素,如果非营养载体是必要的。
  • 维生素混合物的制定考虑到了饮食中的包容率。多用或少用会损害你的实验动物的健康。
  • 对于那些自己准备饮食的人来说,维生素预混剂是向饮食中添加维生素的一种有效方法。
  • 维生素混合物应储存在4摄氏度或更低的接收。如果使用时间超过1个月,建议使用摄氏-20度。
  • 库存和定制维生素混合物的最低订购量为500克。
  • 我们可用的维生素混合物通常用于饮食,并可从库存中获得。
  • 维生素混合物在订购后几天内发货。

我们的公式示例

  • CA.40060 特克拉德维生素混合物
  • CA.40077 AIN-76A维生素混合物
  • TD.94047 AIN-93维生素混合物

矿物混合物

  • 下面所示的常见矿物混合物是以蔗糖为载体的。
  • 如果需要非营养的载体,定制的矿物混合物可以使用纤维素,也可以不使用载体。
  • 我们的矿物质混合物是根据饮食包容率来制定的。多用或少用会损害动物的健康。
  • 对于那些自己准备饮食的人来说,矿物预混料是向饮食中添加矿物质的有效方法。
  • 矿物混合物应存放在干燥、凉爽的环境中,并在收到后一年内使用。库存和自定义矿物混合物的最低订购量为500克。
  • 这些矿物混合物通常用于饮食,并可从库存中获得。
  • 我们的矿物混合物在订货后几天内装运。

公式示例:

  • CA.170915 AIN-76矿物混合物
  • TD.94046 AIN-93g矿物混合物
  • TD.94049 AIN-9300万矿物混合物

其他混合材料(可能从库存中获得,也可能无法从库存中获得):

  • TD.83171不含A,D,E,胆碱的维生素混合物
  • TD.81062基于AIN-76A的缺铁矿物配料
  • TD.79055 AIN-76混合钙磷缺乏矿物
  • TD.98057AIN-93混合钙磷缺乏矿物
  • CA.170760 罗杰斯-哈珀矿物混合物