Wako 分散酶 Dispase CAS 9001-92-7

Wako 分散酶 Dispase CAS 9001-92-7

Dispase分散酶

Dispase分散酶是一类中性金属蛋白水解酶,可以回收生长在Matrigel基质上的细胞。与胰酶、胶原酶相比,它的作用更加温和,所以不会损伤细胞。此外,分散酶也可以用于组织分离。

来源:多粘芽孢杆菌表达的金属蛋白酶
使用指南:推荐浓度为10 U/cm2 BD Matrigel基质,如35 mm的培养皿推荐使用浓度为100U。
保存条件和效期:-20℃下冷冻保存,避免多次冻融。保质期详见产品包装。

Wako 分散酶 Dispase CAS 9001-92-7

383-02281 DISPASEⅡ 分散酶Ⅱ 1 g 9001-92-7
386-02271 DISPASE®Ⅰ 分散酶 10000 PU×6 9001-92-7

Roche公司的Dispase分散酶产品

货号 品名 规格
Roche-04942078001 Dispase® II (neutral protease, grade II) 1 g
Roche-04942078001 Dispase® II (neutral protease, grade II) 100mg

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Wako 014-18331 Acibenzolar-S-methyl Standard 噻二唑素-S-甲基标准品

Wako 014-18331 Acibenzolar-S-methyl Standard 噻二唑素-S-甲基标准品

英文名称:Acibenzolar-S-methyl Standard
中文名称:噻二唑素-S-甲基标准品
品牌:Wako
CAS No.:135158-54-2
储存条件:2-10℃(RT)
纯度:99.0+% (qNMR)

其他农残兽残分析相关产品:

施用于作物上的农药,其中一部分附着于作物上,一部分散落在土壤、大气和水等环境中,环境残存的农药中的一部分又会被植物吸收。残留农药直接通过植物果实或水、大气到达人、畜体内,或通过环境、食物链最终传递给人、畜。

到目前为止,世界上化学农药年产 量近200万吨,约有1000多种人工合成化合物被用作杀虫剂、杀菌剂、杀藻剂、除虫剂、落叶剂等类农药。农药尤其是有机农药大量施用,造成严重的农药污染问题,成为对人体健康的严重威胁。

农药残留对健康的影响

食用含有大量高毒、剧毒农药残留引起的食 物会导致人、 畜急性中毒事故。长期食用农 药残留超标的农副产品,虽然不会导致急性中 毒,但可能引起人和动物的慢性中毒,导致疾病的发生,甚至影响到下一代。

影响农业生产

由于不合理使用农药,特别是除草剂, 导致药害事故频繁, 经常 引起大面积减产甚至绝产,严重影响了农业生产。土壤中残留的长残 效除草剂是其中的一个重要原因。

影响进出口贸易

世 界各国,特别是发达国家对农药残留问题高度重视, 对各种农副产品 中农药残留都规定了越来越严格的限量标准。许多国家以农药残留限量为技术壁垒,限制 农副产品进口,保护农业生产。2000年,欧共体将氰戊菊酯在茶叶中的残留限量从 10毫克/千克降低到0.1毫克/千克,使中国茶叶出口面临严峻的挑战。

农残限量

世界卫生组织和联合国粮 农组织(WHO/FAO)对农药残留限量的定义为 , 按照良好的农业生产(GAP)规范,直接或间接使用农药后,在食品和饲料中形成的农药残留物的最大浓度。目前,中国已制定了79种农药在32种(类 )农副产品中197项农药最高残留限量 (MRL)的国家标准。

农药标准品 (一)

农残专用级别或HPLC级别的农药标准品, 纯度均大于98%。

产品编号 产品名称 中文名称 CAS NO. 包装
205-16281 beta-Trenbolone Standard 三烯酮标准品 10161-33-8 200mg
020-15311 Butylhydroxyanisole Standard (mixture of isomers) 丁基羟基茴香醚标准品 25013-16-5 200mg
086-08241 Hydrocortisone Standard 氢化可的松标准品 50-23-7 200mg
158-02531 Oxacillin Sodium Monohydrate Standard 苯唑西林钠一水合物标准品 7240-38-2 200mg
159-2561 Oxydozanide Standard 五氯柳胺标准品 2277-92-1 200mg
208-16271 Trenbolone Acetate Standard 乙酸去甲雄三烯醇酮标准品 10161-34-9 200mg
159-02681 (5Z)-Orysastrobin Standard (5z)-肟醚菌胺标准品   50mg
011-20051 (Aminomethyl)phosphonic Acid Standard 氨甲基磷酸标准品 1066-51-9 200mg
044-26063 (E)-Dimethylvinphos Standard E-甲基毒虫畏标准品 71363-52-5 50mg
063-04131 (E)-Ferimzone Standard E-嘧菌腙标准品   200mg
139-15891 (E)-Metominostrobin Standard (E)-苯氧菌胺标准品 133408-50-1 100mg
132-15521 (E)-Mevinphos Standard (E)-速灭磷标准品 298-01-1 100mg
167-16691 (E)-Pyrifenox Standard E-啶斑肟标准品 83227-22-9 200mg
166-19841 (E)-Pyriminobac-methyl Standard 嘧草醚标准品 147411-696 200mg
215-01331 (R)-Uniconazole Standard 烯效唑标准品 83657-16-3 50mg
023-12741 (RS)-s-Butylamine Standard (+/-)-1-甲基丙胺标准品 13952-84-6 200mg
045-25231 (Z)-DimethyMnphos Standard (Z)二甲基亚硝胺标准品 67628-93-7 200mg
067-05011 (Z)-Fenpyroximate Standard (z)-唑螨酯标准品 149054-53-5 20mg
066-04121 (Z)-Ferimzone Standard (Z)-嘧菌腙标准品 89269-64-7 200mg
139-15911 (Z)-Metominostrobin Standard (z)-苯氧菌胺标准品 133408-51-2 20mg
139-15531 (Z)-Mevinphos Standard (Z)-速灭磷标准品 33845-4 100mg
160-16701 (Z)-Pyrifenox Standard (Z)-比芬诺标准品 83227-23-0 200mg
163-19851 (Z)-Pyriminobac-methyl Standard (z)-嘧草醚标准品 147411-709 50mg
044-29601 1.1-DiChloro-2.2-bis(4-ethylphenyl)Ethane Standard l,1-二氯-2,2-二(4-乙苯)乙烷标准品 72-56-0 200mg
054-04121 1 2-DIbromoEthane Standard Solution l,2-二溴甲烷标准溶液 106-93-4 1mLx5
133-14831 1-Methylpiperidine Standard 1-甲基哌啶标准品 626-67-5 200mg
141-06501 1-Naphthylacetamide Standard 萘乙酰胺标准品 86-86-2 200mg
148-06511 1-Naphthylacetic Acid Standard a-萘乙酸标准品 86-87-3 200mg
208-11911 2,4,5-T Standard 2,4,5-T标准品 93-76-5 200mg
204-13451 2,4,5-T-butyl Standard 2,4,5-特丁基标准品 93-79-8 200mg
203-15481 2,4,6-Tnchlorophenol Standard 三氧苯酚标准品 32296 200mg
048-29741 2,4-DB Standard 2,4-DB标准品 94-82-6 200mg
045-25591 2,4-DiChloroaniline Standard 2,4-二氯苯胺标准品 554-00-7 200mg
164-18161 2,4-PA-butyl Standard 2,4-聚酰胺-丁基标准品 94-80-4 200mg
045-29371 2,6-Difluorobenzoic Acid Standard 2,6-二氟苯甲酸标准品 385-00-2 200mg
043-29811 2,6-Diisopropylnaphthalene Standard 2,6-二异丙基标准品 24157-81-1 200mg
011-08711 2-Aminobenzimidazole Standard 2-氨基苯并咪唑标准品 93432-7 200mg
169-17871 2-Phenylphenol Standard 邻苯基苯酚标准品 90-43-7 200mg
206-16951 3-(2,4,6-TrimethylphenylsuIfonyl)-1,2,4-triazole Standard 3-(2,4,6三甲基苯基磺酰基)-1,2,4三唑标准品 149591-20-8 S0mg
134-11941 3-(Methylphosphinico)propionic Acid Standard 3-(甲基膦酸基)丙酸标准品 15090-23-0 200mg
085-08571 3-Hydroxycarbofuran Standard 3-羟基呋喃丹标准品 16655-82-6 50mg
046-28441 4,4-Dimethyl-2-oxazolidinone Standard 4,4-双甲基-2-噁唑烷酮标准品 26654-39-7 200mg
030-19511 4-CPA Standard 对氧苯氧乙酸标准品 122-88-3 200mg
086-08501 4-Hydroxybiphenyl Standard 对羟基苯酚标准品 92-69-3 200mg
131-15731 4-Methyl-1,2,3 thiadiazole-5-Carboxylic Acid standard 4-甲基-1,2,3-噻二唑-5-甲酸标准品 18212-21-0 100mg
080-08521 5-Hydroxythiabendazole Standard 5-羟基噻苯咪唑标准品 948-71-0 20mg
022-15251 6-Benzylaminopunne Standard 6-苄氨基嘌呤标准品 1214-39-7 200mg
032-20561 6-Chloropicolinic Acid Standard 2-氯吡啶-6-羧酸标准品 4684-94-0 100mg
016-20361 Abamectin Standard 阿维菌素标准品 71751-41-2 200mg
018-18591 Acequinocyl Standard 灭螨醌标准品 57960_19_7 200mg
011-18601 Acequinocyl-hydroxy Standard 羟基灭螨醌标准品 57960-31-3 200mg
014-16491 Acetamiprid Standard 啶虫脒标准品 160430-64-8 200mg
013-20511 Acetochlor Standard 乙草胺标准品 34256-82-1 100mg
018-19451 Acibenzolar Acid Standard 阿拉酸式苯标准品 35272-27-6 100mg
014-18331 Acibenzolar-S-methyl Standard 阿拉酸式苯-S-甲基标准品 135158-542 200mg
010-20521 Aafluorfen Standard 三氟羚草醚标准品 50594-66-6 200mg
013-15741 ACN Standard ACN标准品 2797-51-5 200mg
018-16651 Acrinathrin Standard 氟丙菊酯标准品 101007-06-1 200mg
017-15521 Alanycarb Standard 棉铃威标准品 83130-01-2 200mg
018-17011 Allethrin Standard 烯丙菊酯标准品 584-792 200mg
019-20611 Allidocfilor Standard 二丙烯草胺标准品 93-71-0 100mg
015-09733 Alloxydim Sodium Standard 禾草灭标准品 55635-13-7 200mg
019-13641 Ametryn Standard 莠灭净标准品 834-128 200mg
011-14941 Amitraz Metabolite HydroChlonde Standard 双甲脒盐酸盐代谢物标准品 51550-40-4 200mg
015-09593 Amitraz Standard 双甲眯标准品 33089-61-1 200mg

Wako 二氧化硅,硅胶,7631-86-9

Wako 二氧化硅,硅胶,7631-86-9

Neo BLUE,1-2mm CAS : 7631-86-9 145-07361 500g

Neo BLUE,2-4mm Discontinued CAS : 7631-86-9 142-07371  500g

Neo BLUE,3-5mm Discontinued CAS : 7631-86-9 149-07381  500g

品牌 产品编号 产品名称 等级 规格 CAS No.
Wako 193-16635 Silica Gel, Small Granular, Mixed(Green) 硅胶,小颗粒,混合(绿色) No grade 500 g 7631-86-9
Wako 192-18285 SilicaGel,LargeGranular,Blue  硅胶,LargeGranular,蓝 500g 7631-86-9
Wako 199-18295 SilicaGel,SmallGranular,Blue  硅胶,SmallGranular,蓝 500g 7631-86-9
Wako 190-18301 SilicaGel,MediumGranular,Blue  硅胶,MediumGranular,蓝 3kg 7631-86-9
Wako 192-18305 SilicaGel,MediumGranular,Blue  硅胶,MediumGranular,蓝 500g 7631-86-9
Wako 197-16655 Silica Gel, Medium Granular, Mixed(Green)  硅胶,中等颗粒,混合(绿色) No grade 500 g 7631-86-9
Wako 199-00625 Silicon Dioxide  二氧化硅 JIS Special Grade 500 g 7631-86-9
Wako 238-01465 Wakogel C-300HG (40 – 60um, 70% up) for Column Chromatography 500 g 7631-86-9
Wako 190-00471 Silica Gel, Medium Granular, Blue  硅胶,中粒,蓝色 3 kg 7631-86-9
Wako 238-01781 Wakogel DX  硅胶Wakogel® DX for Dioxins Analysis 100 g 7631-86-9
Wako 191-00445 Silica Gel, Small Granular, Blue  硅胶,小粒,蓝色 500 g 7631-86-9
Wako 321-38372 Silicon Dioxide, 70nm 25 g 7631-86-9
Wako 148-07378 Neo BLUE,2-4mm No grade 10 kg 7631-86-9
Wako 192-00497 Silica Gel, Medium Granular, Mixed  硅胶,中粒,混合物 12.5 kg 7631-86-9
Wako 190-16645 Silica Gel, Medium Granular, Green  硅胶,中等颗粒,绿色 No grade 500 g 7631-86-9
Wako 198-00477 Silica Gel, Medium Granular, Blue  硅胶,中粒,蓝色 12.5 kg 7631-86-9
Wako 194-16665 Silica Gel, Large Granular, Green  硅胶,大颗粒,绿色 No grade 500 g 7631-86-9
Wako 239-01711 5% Water-impregnated Silica Gel for Environment Analysis 100 g 7631-86-9
Wako 140-07377 Neo BLUE,2-4mm  硅胶,蓝色,2-4mm No grade 13 kg 7631-86-9
Wako 198-16641 Silica Gel, Medium Granular, Green  硅胶,中等颗粒,绿色 No grade 3 kg 7631-86-9
Wako 142-07371 Neo BLUE,2-4mm  硅胶,蓝色,2-4mm No grade 500 g 7631-86-9
Wako 196-00515 Silica Gel, Large Granular, White  硅胶,大粒,白色 No grade 500 g 7631-86-9
Wako 196-08295 Silicon Dioxide  二氧化硅 Practical Grade 500 g 7631-86-9
Wako 191-16675 Silica Gel, Large Granular, Mixed(Green)  硅胶,大颗粒,混合(绿色) No grade 500 g 7631-86-9
Wako 190-09072 Silicon Dioxide, 99.9%  99.9%二氧化硅 No grade 25 g 7631-86-9
Wako 145-07361 Neo BLUE,1-2mm  硅胶,蓝色,1-2mm No grade 500 g 7631-86-9
Wako 190-16667 Silica Gel, Large Granular, Green   硅胶,大颗粒,绿色 No grade 12 kg 7631-86-9
Wako 236-01461 Wakogel C-300HG (40 – 60um, 70% up)   Wakogel® C-300HG (40 – 60um, 70% up)硅胶填充剂 for Column Chromatography 2 kg 7631-86-9
Wako 190-08298 Silicon Dioxide  二氧化硅 Practical Grade 20 kg 7631-86-9
Wako 193-16657 Silica Gel, Medium Granular, Mixed(Green)   硅胶,中等颗粒,混合(绿色) No grade 12 kg 7631-86-9
Wako 196-16647 Silica Gel, Medium Granular, Green  硅胶,中等颗粒,绿色 No grade 12 kg 7631-86-9
Wako 143-07367 Neo BLUE,1-2mm  二氧化硅Neo BLUE,1-2mm No grade 13 kg 7631-86-9
Wako 196-16625 Silica Gel, Small Granular, Green  硅胶,小颗粒,绿色 No grade 500 g 7631-86-9
Wako 196-00635 Silicon Dioxide  二氧化硅 Wako 1st Grade 500 g 7631-86-9
Wako 192-09071 Silicon Dioxide, 99.9% 二氧化硅,99.9% No grade 100 g 7631-86-9
Wako 199-00485 Silica Gel, Medium Granular, White  硅胶,中粒,蓝色 No grade 500 g 7631-86-9
Wako 192-00475 Silica Gel, Medium Granular, Blue  硅胶,中粒,蓝色 500 g 7631-86-9
Wako 193-00525 Silica Gel, Large Granular, Mixed 二氧化硅,大粒,混合物 500 g 7631-86-9
Wako 195-00507 Silica Gel, Large Granular, Blue  硅胶,大粒,蓝色 12.5 kg 7631-86-9
Wako 196-00495 Silica Gel, Medium Granular, Mixed  硅胶,中粒,混合物 500 g 7631-86-9
Wako 232-01468 Wakogel® C-300HG (40 – 60um, 70% up)  硅胶 C-300HG (40 – 60um, 70+%) for Column Chromatography 25 kg 7631-86-9
Wako 199-00505 Silica Gel, Large Granular, Blue  硅胶,大粒,蓝色 500 g 7631-86-9

Wako 294-67001 TRAP/ALP stain kit TRAP/ALP双重染色试剂盒

Wako 294-67001 TRAP/ALP stain kit TRAP/ALP双重染色试剂盒

英文名称:TRAP/ALP stain kit
TRAP/ALP双重染色试剂盒
品牌:Wako
品牌中文简称:和光纯药
储存条件:-20℃
等级:for Pathology Research

品牌 产品编号 产品名称 等级 规格
Wako 294-67001 TRAP/ALP stain kit  TRAP/ALP双重染色试剂盒 for Pathology Research 60次

This product is for research use only. Do not administer it to human.
For enzyme activity staining of tartrate-resistant acid phosphatase and alkaline phosphatase

Normal bone metabolism is based on the balance between bone formation by osteoblasts and bone resorption by osteoclasts. When the balance is disturbed and bone resorption by osteoclasts is abnormally increased, bone mass is reduced leading to osteoporosis. Therefore, various researches have been carried out to understand the mechanism of osteoclast and osteoblast metabolism, and to utilize this knowledge for the treatment of the diseases and for the development of effective drugs.
Today, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) are known as marker enzymes for osteoblasts and osteoclasts, respectively, and these enzymes are used as one of the markers showing the presence of osteoblasts and osteoclasts in tissue sections or cultured cells.
This kit enables you to examine the state of differentiation of bone cells and the cell distribution in bone tissues by observation of the stained images of osteoblasts and osteoclasts in the tissues and cultured cells using ALP/TRAP enzyme activities in the tissue sections and cultured cells.

[Features]

In use, just mix two solutions for the preparation of the colorimetric substrate solution required for staining of acid phosphatase enzyme activity. (three solutions in case of the evaluation of tartrate-resistant acid phosphatase)

Simple steps for staining of alkaline phosphatase enzyme activity by using ALP substrate soln. (premixed).

Double staining can be performed, reddish-purple for active region of acid phosphatase and bluish brown for alkaline phosphatase.

Usable in cultured cells and bone tissue sections (GMA resin embedded section).

[Kit Contents]

Sodium tartrate soln. (x10) … 3 mL x 1 bottle

TRAP substrate soln. A … 30 mL x 1 bottle

TRAP substrate soln. N … 0.3 mL x 1 bottle

Nuclear stain soln. … 10 mL x 1 bottle

ALP substrate soln. (premixed) … 30 mL x 1 bottle

上海金畔生物科技有限公司(www.jinpanbio.com)提供生命科学研究领域系列产品,包括生化试剂、诊断试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;修饰性PEG(Laysan bio、NANOCS、Avanti等进口品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;WAKO日本和光纯药、日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Research diets、Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫诊断试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、老鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

Wako代理 028-17811 BES-H2O2-Ac 过氧化氢特异性荧光探针

Wako代理 028-17811 BES-H2O2-Ac 过氧化氢特异性荧光探针

This product is for research use only. Do not administer it to human.

When deacetylated within the cell by esterase, this deacetylated product (see the reaction below) reacts with hydrogen peroxide by fluorescing. As such, it is ideal as a fluorescent indicator of intracellular hydrogen peroxide. This product reacts with hydrogen peroxide more specifically compared to other fluorescent indicators on the market.

[Advantages]

  • Selectively reacts with hydrogen peroxide
  • Membrane permeability
  • Enables monitoring of intracellular hydrogen peroxide

This product is for research use only. Do not administer it to human.

Highly Selective Fluorescent Probe for Hydrogen Peroxide

[Features]

  1. Highly selectivity toward H2O2
  2. Permeable to cell membrane
  3. Detectability of cell-derived H2O2
  4. Applicable to Molecular Imaging

Reactive oxygen species (ROS) such as superoxide (O2-.),hydrogen peroxide (H2O2), and the hydroxyl radical (HO.) are important mediators of pathological processes in various diseases. 2′,7′-Dichlorofluorescein (DCFH) and its diacetyl derivative have been widely used as fluorescent probes for measuring cell-derived H2O2, but these compounds suffer from the major drawback that they are poorly selective toward H2O2.BES-H2O2 is a probe for cell-derived H2O2 with high selectively. It is applicable to clarifying cell response as well as dynamic function of H2O2 with diseases.

Solubility: Soluble in DMSO, DMF and acetonitrileFluorescence:

  • excitation wavelength: 485 ± 20 nm
  • emission wavelength: 515 ± 20 nm

[References]

  1. Maeda, H., Fukuyasu, Y., Yoshida, S., Fukuda, M. Saeki, K.. Matsuno, H., Yamauchi, Y., Yoshida, K., Hirata, K. and Miyamoto, K. : Angew. Chem. Int. Ed.43, 239 (2004).
  2. Maeda, H., Matsuura, S., Nishida, M., Senba, T., Yamauchi, Y. and Ohmori, H.: Chem. Pharm. Bull.49, 294 (2001).

[Related Products]

Thiol/Selenol Selective Fluorescent Probe

BES-Thio (Wako Cat. #025-15481 (1 mg))

[Fluorescence] excitation wavelength: 495 nm; emission wavelength: 535 nm
Superoxide Selective Fluorescent Probes

BES-So (cell-impermeant) (Wako Cat. #028-16211 (1 mg))

BES-So-AM (Cell-impermeant) (Wako Cat. #021-17801 (1 mg))

[Fluorescence] excitation wavelength: 505 nm; emission wavelength: 544 nm
Highly Selective Fluorescent Probes for Hydrogen Peroxide

BES H2O2 (cell-impermeant)(Wako Cat. #021-16201 (1 mg))

BES H2O2-Ac (cell-permeant)(Wako Cat. #028-17811 (1 mg))

[Fluorescence] excitation wavelength: 485 nm; emission wavelength: 515 nm
LINKProduct Page [BES-H2O2-Ac / BES-H2O2]

LINK  Catalog [Neuroscience Research 2018]