WAKO Iba1抗体 小胶质细胞/巨噬细胞特异性蛋白抗体

WAKO Iba1抗体 小胶质细胞/巨噬细胞特异性蛋白抗体

Iba1标签抗体

Iba1属于17-kDa蛋白,在小胶质细胞和巨噬细胞中特异性表达,是神经系统中小胶质细胞的标记物。

WAKO Iba1抗体 小胶质细胞/巨噬细胞特异性蛋白抗体

Wako 013-27691 Anti Iba1, Rabbit(for Paraffin Section)  小胶质细胞/巨噬细胞特异性蛋白兔源抗体(石蜡切片) for Immunochemistry 50 μg
Wako 016-20001 Anti Iba1,Rabbit (for Western Blotting)  小胶质细胞/巨噬细胞特异性蛋白抗体(免疫印迹) for Immunochemistry 50 ug
Wako 019-19741 Anti Iba1, Rabbit (for Immunocytochemistry)  小胶质细胞/巨噬细胞特异性蛋白抗体(免疫组化) for Immunochemistry 50 ug
Wako 013-27593 Anti Human Iba1, Monoclonal Antibody(NCNP27)  抗人 Iba1,单抗(NCNP27) for Immunochemistry 50 ul
Wako 017-27591 Anti Human Iba1, Monoclonal Antibody(NCNP27)  抗人 Iba1,单抗(NCNP27) for Immunochemistry 10 ul
Wako 012-26723 Anti Iba1, Monoclonal Antibody(NCNP24)  抗Iba1,单克隆抗体(NCNP24)(鼠源) for Immunochemistry 10 ul
Wako 016-26721 Anti Iba1, Monoclonal Antibody(NCNP24)  抗Iba1,单克隆抗体(NCNP24)(鼠源) for Immunochemistry 50 ul
Wako 016-26461 Anti Iba1, Rabbit, Biotin-conjugated  小胶质细胞/巨噬细胞特异性蛋白抗体(结合生物素) for Immunochemistry 100 ul
Wako 013-26471 Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated  小胶质细胞/巨噬细胞特异性蛋白抗体(结合红色荧光素635) for Immunochemistry 100 ul

注意: 该试剂仅供实验、研究使用,不能用作「医疗药品」、「食品」、「家庭用品」等。

016-26721不可用于小鼠免疫荧光,免疫荧光仅能做大鼠,如果客户能接受兔源建议用019-19741做免疫荧光,染鼠脑的工作浓度1:500-1:1000(即使是小鼠)。可用于免疫组化-石蜡切片(IHC-P)、免疫组化-冰冻切片(IHC-Fr)试验。Appearance :Liquid 液体

免疫荧光(人、小鼠、大鼠),一抗使用019-19741抗体,二抗使用结合荧光素色的就可以。

保存方法:Storage:Store at -20 degrees C. After opening, aliquot contents and freeze at -20 degrees C.

在-20℃下储存。打开后,等分内容物并在-20℃下冷冻。 反复冻存影响抗体性能,最好是分装。

016-20001  Anti Iba1,Rabbit (for Western Blotting)

适用实验:免疫印迹实验(Western blotting)

Preparation : Purified by the antigen affinity chromatography from rab-bit antisera and prepared in TBS solution. Contains no pre-servatives and stabilizers.

Specificity : Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte.

Reactive with human, mouse and rat Iba1. 与人,小鼠和大鼠Iba1反应

Working Concentration : Westernblot 0.5-1 μg/mL Storage : Keep at - 20℃.

After opening, aliquot contents and freeze at - 20℃.

Package : 50 μg(100 μL)

Wako Cat.NO:019-19741是抗Iba1,兔源(免疫细胞化学):用生物素或红色荧光染料(如Cy5 TM)标记。

以下图片使用的是Biotin和Cy5TM红色荧光标记的Iba1抗体。

blob.png

◆优点

·    无需二抗: 缩短试验时间!

·      低背景:能获得高分辨率的数据!

·      经免疫组化验证: 提供高质量产品!

◆使用示例

(1)Anti Iba1, Rabbit, Biotin-conjugatedblob.png

与使用二抗相比,背景值更低,小胶质细胞更清晰

■ 实验条件
○样品:7周 的Winstar大鼠或者7周的ICR小鼠,

大脑皮质层冷冻切片

○染色方法法:ABC法+DAB染色

○抗体浓度:1/200

2)Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugatedblob.png

与使用二抗相比,效果相同

■实验条件

○样品:7周的Winstar大鼠或者7周的ICR小鼠大脑皮质层的冷

冻切片

○抗体浓度: 1/200

数据提供:

Sanagi ,T. , Ichinohe  ,N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.(日本国家研究精神病学,神经医疗研究中心,佐柳老师、一户老师、髙坂老师)

◆特性

Anti   Iba1, Rabbit,

Biotin-conjugated

Anti Iba1, Rabbit, Red

Fluorochrome(635)-conjugated

标记物 Biotin生物素 Cy5TM   红色荧光标记

(Ex=634nm, Emi=654nm)

抗原 合成肽(Iba1的C末端的序列)
缓冲液 含0.05% 叠氮化钠的PBS
类别 兔IgG
特异性 小鼠、大鼠、狨 小鼠、大鼠
应用 IHC免疫组化(1:200-2,000)

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产品编号 产品名称 规格 产品等级
013-26471 Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated小胶质细胞/巨噬细胞特异性蛋白抗体(结合红色荧光素635) 100μl for Immunochemistry
016-26461 Anti Iba1, Rabbit, Biotin-conjugated小胶质细胞/巨噬细胞特异性蛋白抗体(结合生物素) 100μl for Immunochemistry
017-26491 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) FITC-conjugated 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64)、FITC相结合 100μ for Immunochemistry
010-26481 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64)Biotin-conjugated 抗磷酸化α突触核蛋白、单克隆抗体(PSYN#64)、生物素相结合 100μ for Immunochemistry
015-25191 Anti Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 抗磷酸化α-突触核蛋白单克隆抗体 (pSyn#64) 50ul for Immunochemistry
016-20001 ANTI IBA1,RABBIT (FOR WESTERN BLOTTING) 小胶质细胞/巨噬细胞特异性蛋白抗体(免疫印迹) 50μg for Immunochemistry
019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 小胶质细胞/巨噬细胞特异性蛋白抗体(免疫组化) 50μg for Immunochemistry