Maltose Phosphorylase (MPL-EP)マルトースホスホリラーゼ(MPL-EP)
- 臨床診断用酵素
臨床検査において、α-アミラーゼおよび無機リン酸の測定に利用されます。
| 由来 | recombinant E. coli |
|---|---|
| 系統名 | Maltose : orthophosphate 1-β-D-glucosyltransferase |
| EC 番号 | 2.4.1.8 |
| 反応式 | Maltose + Orthophosphate →→→ D-Glucose + β-D-Glucose 1-phosphate |
仕様
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧10 U/mg | |
| Stabilizer | lactose, ethylenediaminetetraacetic acid (EDTA) | |
| Storage condition | below -20℃ |
特性
| Molecular weight | ca. 220 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 90 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10-3M (phosphate) |
| 3.4×10-3 M (phosphate) | |
| 8.3×10-3M (arsenate) | |
| pH Optimum | 6.5–7.5 |
| pH Stability | 5.5–8.0 |
| Optimum temperature | 45–50°C |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at least four weeks |
| Inhibitors | Hg2+，Ag+，Zn2+，Cu2+ |
アプリケーション
参考文献
- Hiruma, M. et al., Nippon Nogeikagaku Kaishi, 70, 773–780 (1996).
- Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).
| PRODUCT名称 | CODE编号 | ORIGIN来源 | Activity活力单位（more than） |
| Alkaline phosphatase(ALP) | calf intestinal mucosa | 6000 U/mg protein | |
| Catalase(CAT) | 61152 | bovine liver | 12,000U/mg lyophilizate |
| Catalase(CAT-R) | 61164 | recombinant protein | 12,000U/mg lyophilizate |
| Cholesterol esterase(CHE-XE) | 60468 | recombinant E.coli | 5 U/mg lyophilizate |
| Cholesterol oxidase(CHO-CE) | 60266 | recombinant E.coli | 5 U/mg lyophilizate |
| Cholesterol oxidase(CHO-PEWL) | 60271 | recombinant E.coli | 200U/ml |
| Creatinase(C2-AE) | 60108 | recombinant E.coli | 9 U/mg lyophilizate |
| Creatinase(C2-AT) | 60118 | recombinant E.coli | 9 U/mg lyophilizate |
| Creatininase(C1-E) | 60111 | recombinant E.coli | 500 U/mg lyophilizate |
| Fructosyl-amino acid oxidase(FAOX-TE) | 60242 | recombinant E.coli | 0.5 U/mg lyophilizate |
| Fructosyl-amino acid oxidase(FAOD-E) | 60273 | recombinant E.coli | 4 U/mg lyophilizate |
| Fructosyl-peptide oxidase(FPOX-CE) | 60123 | recombinant E.coli | 4 U/mg lyophilizate |
| Fructosyl-peptide oxidase(FPOX-EE) | 60454 | recombinant E.coli | 0.5 U/mg lyophilizate |
| L-Fucose dehydrogenase(FDH-E) | 60952 | recombinant E.coli | 10 U/mg lyophilizate |
| Glucoamylase(GLA) | 60125 | Rhizopus.sp | 40 U/mg lyophilizate |
| Glucose oxidase(GOD-R) | 61240 | Aspergillus niger | 100 U/mg lyophilizate |
| Glucose-6-phosphate dehydrogenase(GPD-M) | 61153 | microorganism | 60 U/mg lyophilizate |
| α-Glucosidase(alphaGLS-SE) | 60241 | recombinant E.coli | 10 U/mg lyophilizate |
| β-Glucosidase(betaGLS-A) | 60126 | sweet almond | 10 U/mg lyophilizate |
| L-Glutamate oxidase(GLOD) | 60239 | recombinant E.coli | 3 U/mg lyophilizate |
| Glutaminase(GLN) | 60463 | microorganism | 4 U/mg lyophilizate |
| Glutamine synthetase(GST) | 61222 | microorganism | 7 U/mg lyophilizate |
| Histamine dehydrogenase(HDH-E) | 60477 | recombinant E.coli | 1 U/mg lyophilizate |
| 3α-Hydroxysteroid dehydrogenase(3αHSD-EH) | 61224 | recombinant B.choshinensis | 30 U/mg lyophilizate |
| Lactate dehydorgenase(LDH-P) | 61170 | porcine heart | 150 U/mg lyophilizate |
| Lactate dehydorgenase(LDH-R) | 61171 | rabbit muscle | 300 U/mg lyophilizate |
| Malate dehydrogenase(MDH-P) | 61169 | porcine heart | 100 U/mg lyophilizate |
| Maltose 1-epimerase(MER) | 60133 | Lactobacillus brevis | 20 U/mg lyophilizate |
| Maltose phosphorylase(MPL-EP) | 60233 | recombinant E.coli | 10 U/mg lyophilizate |
| β-Phosphoglucomutase( βPGM-EP) | 60189 | recombinant E.coli | 20 U/mg lyophilizate |
| Pyranose oxidase(PROD-E) | 60274 | recombinant E.coli | 5 U/mg lyophilizate |
| Sarcosine oxidase(SOD-TE) | 60105 | recombinant E.coli | 15 U/mg lyophilizate |
| Sorbitol dehydrogenase(SDH-E) | 60462 | recombinant E.coli | 20 U/mg lyophilizate |
| Sucrose phosphorylase | 60122 | recombinant E.coli | 50 U/mg lyophilizate |
| Urease(URE) | 61156 | jack bean | 100 U/mg lyophilizate |
| Uricase(U-TE) | 60199 | recombinant E.coli | 4 U/mg lyophilizate |
| Biotinylated Luciferase(BLU-Y) | 60407 | recombinant E.coli | |
| Luciferase(LUC-H) | 61314 | recombinant E.coli | |
| Luciferase(LUC-T) | 61315 | recombinant E.coli | |
| Acid protease(ACP) | 60743 | Aspergillus saitoi | 22,000U/g |
| Tannase(TAH) | 60544 | Aspergillus Oryzae | 500U/g |