Phos-tag™ SDS-PAGE相关产品

Phos-tag™ SDS-PAGE相关产品Phos-tag SDS-PAGE相关产品

◆Reagents for Phos-tag™ SDS-PAGE gel preparation

 

Product Name

Pkg. Size

Wako

Cat. No.

Use Application

30 w/v% Acrylamide/Bis Mixed solution(29:1)

500 mL

015-25635

Ready-to-use"Solution A".30%T, 3.3%C

Agarose H(High-strength type)

1 g

315-01203

High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 – 1% and a separation range of 1 – 200 kbp.

10 g

319-01201

25 g

317-01202

10% SDS Solution

100 mL

311-90271

Ready-to-Use "Solution D"

500 mL

313-90275

Manganese(Ⅱ) Chloride Tetrahydrate,
  99.0+ %(Titration)

25 g

134-15302

for Molecular Biology Please use for preparation of "Solution F"

100 g

136-15301

Zinc Chloride

25 g

268-01902

for Molecular Biology. Please use for preparation of "Solution M"

Bis-Tris

100 g

345-04741

Please use for preparation of "Solution N"

Sodium Hydrogensulfite〈JIS   Special Grade〉

25 g

196-01372

Please use for preparation of "Solution O"

100 g

198-01371

Sodium Hydrogensulfite
  〈For Molecular Biology〉

100 g

190-16461

MOPS

100 g

345-01804

Please use for preparation of "Solution P"

250 g

341-01801

MOPS〈For Molecular Biology〉

100 g

341-08241

500 g

343-08245

Separating Gel Buffer Solution (x4)

250 mL

192-11041

Ready-to-Use mixed solution of“Sol. B”and“Sol. D”for preparation of Resolving Gel. Contains SDS.

Stacking Gel Buffer Solution (x4)

250 mL

199-11051

Ready-to-Use mixed solution of“Sol.C”and“Sol. D”for preparation of Stacking Gel. Contains SDS.

N,N,N',N'-Tetramethylethylenediamine (TEMED)

25 mL

205-06313

for Electrophoresis

10 w/v% Ammonium Peroxodisulfate Solution
  (Ammonium Persulfate Solution)

25 mL

019-15922

Ready-to-Use“Solution G”



Premixed Buffers


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

Running Buffer Solution (x10)

1 L

184-01291

Ready-to-Use concentrated "Solution H"

SDS-PAGE 10x Running Buffer

1 L

312-90321

Ready-to-Use concentrated "Solution H"

5 L

318-90323

SDS-PAGE Buffer, pH 8.5

5 L

192-16801

Ready-to-Use "Solution H", 1×buffer.

Tricine Running Buffer Solution (×10)

1 L

200-17071

Composition: 0.5 M Tris/0.5 M Tricine/1% SDS

Sample Buffer Solution (2ME+) (×2)

25 mL

196-11022

Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol

Sample Buffer Solution
  with 3-Mercapto-1,2-propanediol (×2)

25 mL

196-16142

Laemmli Sample Buffer containing   3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME

AquaBlot™ 10×High Efficiency Transfer Buffer

1 L

015-26213

Can use it to transfer to a

menbrane with high efficience.



◆Enzyme for Dephosphorylation


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

Alkaline Phosphatase(for   Biochemistry)

50 U

018-10693

Applicable to dephosphorylation of proteins

100 U

012-10691



◆Reagents for Staining


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

Quick CBB Plus

250 mL

174-00553

Ready-to-Use Sol. K. Fixing and destaining procedure are not required.
No organic solvents are necessary.
Protein bands are stained in 10 minutes.

1 L

178-00551

Quick-CBB
  ・Staining solution A: 1L x 1
  ・Staining solution B: 1L x 1

2 L

299-50101

By mixing staing solution A and B,   ready-to-Use Sol. K

Silver Stain MS Kit

20 tests

299-58901

Modified proteins by glycosilation and/or phosphorylation can be detected sub-nanogram level on the electrophoretic gel.

Silver Stain Kit Wako

for 10 gels

299-13841

50~100 times more sensitive than CBB method.

Silver Stain Ⅱ Kit Wako

for 10 gels

291-50301

This kit contains Stopper, which can be adjusted the staining strength.

Negative Gel Stain MS Kit

20 tests

293-57701

Applicable to mass analysis and Western blot



Protein Size Marker


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

WIDE-VIEW Prestained Protein Size Marker Ⅲ

25 μL

236-02463

A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less distorted.

500 μL

230-02461

500 μL x3

234-02464



Positive Control(for confirmation of separation capacity of Phos-tag™ SDS-PAGE)


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

α-Casein, from BovineMilk, Dephosphorylated

1 mg

038-23221

Mixture of phosphylated and dephosphorylated α-casein

10 mg

034-23223



◆Electrophoresis Apparatus ・ Precast Gels


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

EasySeparator

1 unit

058-07681

An electrophoresis tank for SuperSep precast polyacrylamide gels.

SuperSep Ace, 6%, 13 wells

10 gels

195-15171

Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation.
Expired in 9 months after manufacture

SuperSep Ace, 7.5%, 13 wells

10 gels

198-14941

SuperSep Ace, 7.5%, 17 wells

10 gels

191-14931

SuperSep Ace, 10%, 13 wells

10 gels

195-14951

SuperSep Ace, 10%, 17 wells

10 gels

192-14961

SuperSep Ace, 12.5%, 13 wells

10 gels

199-14971

SuperSep Ace, 12.5%, 17 wells

10 gels

196-14981

SuperSep Ace, 15%, 13 wells

10 gels

193-14991

SuperSep Ace, 15%, 17 wells

10 gels

190-15001



◆Reagents for Western Blotting


Product Name

Pkg. Size

Wako

Cat. No.

Use Application

ImmunoStar LD*
・Luminescence solution A
・Luminescence solution B

200 cm2

296-69901

Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate.
Not available for sale in the US and Europe.

1,000 cm2

292-69903

2,000 cm2

290-69904

ImmunoStar Zeta*

200 cm2

291-72401

Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal.

1,000 cm2

297-72403

2,000 cm2

295-72404

ImmunoStar Basic*

200 cm2

295-75101

A cost-effective, stable and long-lasting product.

2,000 cm2

291-75103

5,000 cm2

299-75104

Immuno Enhancer

2 assays

294-68601

Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA

10 assays

290-68603

40 assays

298-68604

* : Not available for sales in the US and Europe.