鼠源Iba1抗体,无标签,单克隆抗体(NCNP24) Anti Iba1, Monoclonal Antibody (NCNP24)

鼠源Iba1抗体,无标签,单克隆抗体(NCNP24)                              Anti Iba1, Monoclonal Antibody (NCNP24)小胶质标记

鼠源无标签抗Iba1,单克隆抗体(NCNP24)



  Iba1是在小胶质细胞和巨噬细胞中高度表达的蛋白质,分子量约17 kDa。 此蛋白质通常称为中枢神经系统中的小胶质细胞标记物。

  最近,小胶质细胞已被广泛研究,其负责中枢神经系统中的免疫功能,并且与各种疾病相关,如神经变性疾病,精神错乱,脑肿瘤和感染。

鼠源Iba1抗体,无标签,单克隆抗体(NCNP24)                              Anti Iba1, Monoclonal Antibody (NCNP24)

类别:Monoclonal

应用物种:IHC(小鼠、大鼠和狨猴冰冻切片,DABlocking)(1:500-2,000),

应用物种IHC(大鼠冰冻切片,fluorescent)(1:100)

◆特点


● 免疫组化评估

● 用于多色免疫染色

● 具有高特异性和低背景的单克隆抗体



应用 – 免疫组织化学染色


鼠源Iba1抗体,无标签,单克隆抗体(NCNP24)                              Anti Iba1, Monoclonal Antibody (NCNP24)


使用抗Iba1,单克隆抗体(FUJIFILM Wako产品编号:012-26723)小胶质细胞的定位信号清晰,与抗Iba1,兔多克隆抗体的效果相同(FUJIFILM Wako产品编号:019-19741、019-19741)。



实验条件


● 样品:7周龄大鼠,7周龄小鼠或成年狨猴的大脑皮质

● 切片:50 μm冰冻切片(小鼠和大鼠),40 μm冰冻切片(狨猴)

● 染色法:ABC法+DAB染色

● 抗体浓度= 1:500


数据提供


Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan. 


抗体信息


抗Iba1,单克隆抗体(NCNP24)

抗原

合成肽(Iba1的C末端)

克隆号

NCNP24

子类

小鼠IgG1

浓度

1.3 mg/mL

Buffer缓冲液

50%甘油/ TBS,0.05%叠氮化钠

物种反应性

小鼠,大鼠,狨猴

应用

免疫组织化学(冷冻切片)(1:500-2,000)

欲了解相关产品信息请点击文字:

兔源Iba1抗体,有标签

兔源Iba1抗体,无标签


欲了解相关知识请点击文字:

巨噬细胞/小胶质细胞特异性蛋白抗体Iba1的应用

小胶质细胞研究动向与新型Iba1标签抗体

欲了解相关资料请点击文字:

Wako神经生物学抗体清单

巨噬细胞/小胶质细胞Iba1抗体

相关资料


鼠源Iba1抗体,无标签,单克隆抗体(NCNP24)                              Anti Iba1, Monoclonal Antibody (NCNP24)

小胶质细胞产品目录



◆相关产品

产品编号

产品名称

中文名称

包装

储存条件

016-26461

Anti Iba1, Rabbit, Biotin-conjugated

抗Iba1,兔,生物素缀合

100 μL

保存在2-10℃

013-26471

Anti Iba1, Rabbit, Red Fluorochrome   (635)-conjugated

抗Iba1,兔,红色荧光染料(635)结合

100 μL

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Cougnoux, A., Fellmeth, M., Gu, T., Davidson, C. D., Gibson, A. L., Pavan, W. J., & Porter, F. D. (2019). Maternal immune activation modifies the course of Niemann-pick disease, type C1 in a gender specific manner. Molecular genetics and metabolism.

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Groves, A., Kihara, Y., Jonnalagadda, D., Rivera, R., Kennedy, G., Mayford, M., & Chun, J. (2018). A functionally defined in vivo astrocyte population identified by c-Fos activation in a mouse model of multiple sclerosis modulated by S1P signaling: immediate-early astrocytes (ieAstrocytes). eNeuro, 5(5).

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Cai, W., Wang, J., Hu, M., Chen, X., Lu, Z., Bellanti, J. A., & Zheng, S. G. (2019). All trans-retinoic acid protects against acute ischemic stroke by modulating neutrophil functions through STAT1 signaling. Journal of neuroinflammation, 16(1), 175.

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Feng, T., Yamashita, T., Shang, J., Shi, X., Nakano, Y., Morihara, R., … & Matsumoto, N. (2019). Clinical and Pathological Benefits of Edaravone for Alzheimer’s Disease with Chronic Cerebral Hypoperfusion in a Novel Mouse Model. Journal of Alzheimer's Disease, (Preprint), 1-13.

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Chen, Y. J., Nguyen, H. M., Maezawa, I., Jin, L. W., & Wulff, H. (2018). Inhibition of the potassium channel Kv1. 3 reduces infarction and inflammation in ischemic stroke. Annals of clinical and translational neurology, 5(2), 147-161.

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Michaelis, K. A., Norgard, M. A., Levasseur, P. R., Olson, B., Burfeind, K. G., Buenafe, A. C., … & Marks, D. L. (2019). Persistent Toll-like receptor 7 stimulation induces behavioral and molecular innate immune tolerance. Brain, behavior, and immunity, 82, 338-353.

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Surrao, D. C., Greferath, U., Chau, Y. Q., Skabo, S. J., Huynh, M., Shelat, K. J., … & Liu, Q. (2017). Design, development and characterization of synthetic Bruch’s membranes. Acta biomaterialia, 64, 357-376.