LBIS® High Molecular Adiponectin-Mouse/Rat
LBIS® 小鼠/大鼠 高分子量脂联素 ELISA 试剂盒
Adiponectin（脂联素）是脂肪细胞分泌的一种细胞因子。作为脂肪细胞因子，控制脂肪代谢和胰岛素感受性、是抗糖尿病、抗动脉粥样硬化、抗炎症的重要物质。血液中的脂联素通过聚集单聚体形成３聚体、６聚体或者是 12-18 聚体。三聚体（LMW）通过胶原三螺旋链的非共价相互作用以及球状体 C1q 域的疏水相互作用形成。三聚体聚集形成六聚体（MMW）或者更大的多聚体（HMW）。
Adiponectin 与各种各样的生长因子相结合有明显的亲和性，将其隔离能影响细胞的生长、血管新生和细胞组织的重建。血液中 HMW 的测定值除了表示总脂联素以外，同时明确地反映出 BMI 和性别、体重减轻的影响、糖耐量、肝脏的胰岛素感受性、代谢综合征和2型糖尿病。预计 HMW 的测定比起总脂联素的测定，对于代谢综合征和 DM2 的分析更有帮助。
LBIS® 的此款试剂盒只用于测定高分子 Adiponectin。
◆特点
● 短时间测定（总的反应时间：４小时）
● 微量样品可测
● 使用对环境无害的防腐剂
● 全部试剂均为液体，可直接使用
● 精密的测定精度和高再现性
◆构成
|
组成部分 |
状态 |
容量 |
|
(A) 抗体固相化 96 孔板 |
洗净后使用 |
96 wells（8×12）/1块 |
|
(B) 标准溶液（2,000 ng/mL） |
稀释后使用 |
200 μL/1 瓶 |
|
(C) 缓冲液 |
即用 |
60 mL/1 瓶 |
|
(D) HRP标识抗脂联素抗体 |
稀释后使用 |
100 μL/1 瓶 |
|
(F) 显色液（TMB） |
即用 |
12 mL/1 瓶 |
|
(H) 反应终止液（1M H2SO4）※小心轻放 |
即用 |
12 mL/1 瓶 |
|
( I ) 浓缩洗净液（10×） |
稀释后使用 |
100 mL/1 瓶 |
|
封板膜 |
3 张 |
|
|
使用说明书 |
1 份 |
◆交叉反应
|
动物种类 |
对象物质 |
反应性和交叉率(%) |
|
Mouse |
Adiponectin(HMW) |
100 |
|
Adiponectin(Hexamer) |
<5 |
|
|
Adiponectin(Trimer) |
不存在交叉反应 |
|
|
Adiponectin(Monomer) |
不存在交叉反应 |
|
|
MCH |
不存在交叉反应 |
|
|
TNF-α |
不存在交叉反应 |
|
|
IFN-γ |
不存在交叉反应 |
|
|
Insulin |
不存在交叉反应 |
|
|
Leptin |
不存在交叉反应 |
|
|
Rat |
Adiponectin(HMW) |
100 |
|
Adiponectin(Monomer) |
不存在交叉反应 |
|
|
TNF-α |
不存在交叉反应 |
|
|
IFN-γ |
不存在交叉反应 |
|
|
Insulin |
不存在交叉反应 |
|
|
Leptin |
不存在交叉反应 |
※交叉率浓度为1,000 ng/mL
◆样品信息
小鼠/大鼠的血清•血浆•培养液
50 μL/well（稀释样品）
※血浆采血建议使用肝素处理
※正常样品的稀释倍数为50倍（～25倍）
◆测量范围
3.13～200 ng/mL（标准曲线范围）
78.25～5,000 ng/mL（25倍稀释样品）
0.1565～10 μg/mL（50倍稀释样品）
◆Validation data
精度测试（组内变异）
|
样品 |
A |
B |
|
1 |
29.5 |
129 |
|
2 |
30.7 |
125 |
|
3 |
29.8 |
128 |
|
4 |
29.0 |
126 |
|
5 |
29.6 |
126 |
|
mean |
29.7 |
127 |
|
SD |
0.631 |
1.89 |
|
CV(%) |
2.12 |
1.49 |
单位：ng/mL
重复性测试（组间变异）
|
测量日/样品 |
C |
D |
E |
|
第０天 |
196 |
126 |
62.5 |
|
第１天 |
192 |
130 |
59.1 |
|
第２天 |
196 |
125 |
60.7 |
|
第３天 |
190 |
125 |
60.3 |
|
mean |
193 |
127 |
60.7 |
|
SD |
2.63 |
2.27 |
1.41 |
|
CV(%) |
1.36 |
1.79 |
2.33 |
单位：ng/mL n=2
加标回收测试
样品H
|
添加量 |
实测值 |
回收量 |
回收率(%) |
|
0 |
68.5 |
– |
– |
|
35.0 |
103 |
34.5 |
98.6 |
|
65.0 |
132 |
63.5 |
97.7 |
|
95.0 |
165 |
96.9 |
102 |
单位：ng/mL n=2
样品I
|
添加量 |
实测值 |
回收量 |
回收率(%) |
|
0 |
23.3 |
– |
– |
|
18.0 |
40.3 |
17.0 |
94.4 |
|
26.0 |
50.6 |
27.3 |
105 |
|
32.0 |
55.5 |
32.2 |
101 |
单位：ng/mL n=2
稀释直线性测试
用稀释缓冲液分３次连续稀释２个血清样品的测量结果，直线回归方程的 R2 在 0.9987~0.9993 之间。
相关资料
 |
 |
 |
| 说明书 |
ELISA试剂盒选择指南①② |
ELISA试剂盒选择指③④ |
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