UniWells™ Horizontal Co-Culture Plate

UniWells™ Horizontal Co-Culture Plate is an ideal co-culture device that connects two wells laterally.
The horizontal connection provides easy viewing of cells in both wells simultaneously using various types of microscopes.
It is a versatile tool for studying cell-cell interactions such as transport, migration, and invasion.

Instruction

Features
Excellent points
Kit Contents
Example of use – Uptake of exosomes –
Instructions for use
Medium volume
Product List
Related Information

Features

Simultaneous observation using a time-lapse microscope
No more filter clogging
Cells are cultured under the same condition (used same material on the bottom)
Filters of any membrane types and pore sizes are usable
Both wells are independent (connect in a free combination)

Excellent points

Both cells can be cultured under the same medium volume

Conventional co-culture plate

Medium volume in A is more than in B and cell secreted factors A are diluted in B.
UniWells™

Medium volume are the same between A and B

Filter is prevented from being clogged by cells

Conventional co-culture plate

The filter is clogged by cells cultured in the upper well, which leads to interfering with the migration of cell secreted factors between the upper and lower wells.
UniWells™

The filter is NOT clogged by cells.

Kit Contents

UniWells™ Horizontal Co-Culture Plate (384-14421)

Main Body A	・・・2
Main Body B	・・・2
Cover	・・・4
Common Cover	・・・4
Adapter	・・・1
O-ring	・・・2
[Note] The filters are sold separately

Example of use – Uptake of exosomes –

UniWells™ Horizontal Co-Culture Plate enables the observation that exosomes derived from cell B permeate the filter and are uptaken by cell A.

Comparison of permeability testing of exosome between conventional co-culture plate and UniWells™

Cells were seeded to be the same cell numbers into the upper well in conventional co-culture plate and one side well in Uniwells™.
Co-culture started after 24 hours from cell seeding.
Exosome densities of the cell side and medium side were analyzed.

Instructions for use

Connected use

Wells are connected by fitting a filter, O-ring and cover.

※The filters are sold separately.

Connection methods are as below;

A)Connect the wells cultured independently by aspirating the culture solution once.
B)Connect first and increase the volume of the culture solution to achieve co-culture
Single use

Fit a common cover and cover to a well.

Use in a microscope

Set wells to the adapter provided.

※The adapter is attached to the main unit.

Medium volume

A medium volume of less than 400 μL is not shared between the wells.

The maximum medium volume on each well is 1.8 mL.

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