Serum-Free Cell Freezing Medium
BAMBANKER™ & BAMBANKER™ Direct
BAMBANKER™ is a serum-free medium for long-term freezing at -80°C and preservation of valuable culture cells such as tumor cells and normal cells.
BAMBANKER™ Direct is improved operability. It doesn’t require centrifugation for cell collecting.
User report 1 by: Shota Moriya, Department of Biochemistry, Tokyo Medical University
User report 2 by:Dr. Atsunori Sato of Tagawa Laboratory, School and Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology
- User Report 1Jul. 01, 2017
- User Report 2Feb. 01, 2019
- Features
- Operating Procedure
- Sterility test
- Application
- Comparison
- Precautions
- References
- Product List
- Related Information
Features
- Ready-to-use medium for preservation of cells
- Usable without diluting
- NO program freezer is required
- Rapid and long-term freezing and preservation in a deep freezer (-80°C)
- Serum-free
Operating Procedure
BAMBANKER™
BAMBANKER™ Direct
Sterility test
| Endotoxin: | Chromogenic substrate method |
| Mycoplasma: | Fluorescent antibody method |
| Fungi and bacteria: | As per Japanese Pharmacopoeia |
| **: Certificate of analysis will be issued upon request. | |
Application
Cell lines
| •201B7 (human iPS cell line) | •3T3-L1 (mouse preadipocyte cell line) | •A431 (human squamous cell carcinoma cell line) | |
| •BAEC (bovine aortic vascular endothelial cell line) | •Balb/3T3 (mouse fibroblast cell line) | •C2C12 (mouse skeletal muscle cell line) | |
| •Daudi (human B cell line) | •ECV 304 (human umbilical vein endothelial cell line) | •H295R (adrenal cortex cells) | |
| •HEK 293 (human embryonic kidney cell line) | •HEK293T (human fetal kidney cell line) | •HeLa (human cervical cancer cell line) | |
| •HeLa S3 (human cervical cancer cell line) | •HepG2 (human liver cancer cell line) | •HFF (human normal fibroblast cell line) | |
| •Huh7 (human liver cancer cell line) | •Jurkat (human leukemia T cell line) | •K562 (human chronic myelogenous leukemia cell line) | |
| •KATO III (human gastric cancer epithelial cell line) | •KLM-1 (human pancreatic cancer cell line) | •MDCK (canine kidney tubular epithelial cell line) | |
| •DT40 (derived from chicken B cells) | •NIH3T3 (mouse fetal skin cell line) | •OKT4 (mouse hybridoma) | |
| •OP9 (mouse myeloid stromal cells) | •P3U1 (mouse myeloma cell line) | •PANC-1 (human pancreatic cancer cell line) | |
| •PC12 (rat-derived adrenal pheochromocytoma cell line) | •RPE (human membrane epithelial cell line) | •SNL (mouse fetal fibroblasts) | |
| •TSU-Pr1 (human prostate cancer cell line) | •Vero (African green monkey kidney cell line) | •Mouse ES cell line | |
| •Human immortalized myocytes | •Human immortalized myocytes | ||
Primary cells
| •MEF (mouse fetal fibroblasts) | •Human γδ T cells | •Human peripheral blood-derived activated lymphocytes | |
| •PBMC (peripheral blood mononuclear cells) | •Mouse spleen-derived activated lymphocytes | ||
Comparison
Comparison of preservation efficiency of mouse fibroblast strain
| BAMBANKER™ | Self prepared solution | ||||||||||||||||
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Better cell viability was obtained using BAMBANKER™ than self-prepared preservative solution.
Comparison of Bambanker™ with other relevant products
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[Cryopreservation Test] Result *1: Storage period of Cell Line at -80ºC |
Media tested 1. Bambanker™ (LYMPHOTEC Inc.) 2. Medium with serum (Company A) 3. Serum-free Medium (Company A) |
Precautions
- Use only for research purposes, not for human use.
- Prior to using this medium, perform the confirmation test on the cells under study.
- The manufacturer shall not be responsible for any accident or damage caused by the use of this product.
- If you have any questions on the use of this medium, please contact the local distributor.
References
- T.Hikichi, et al; Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 25, 46-53(2007)
- Durruthy-Durruthy, J. et al. : Nature Genetics, 48, 44-52 (2016).
- Sugii, S. et al. : Nature Protocols, 6, 346–358 (2011).
- Zaidi, SK. et al. : Proc Natl Acad Sci U S A, 104, 19861–19866 (2007).
Product List
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Related Information
Category
- Cell Culture
- Animal Cell Culture
- Cell Preservation
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