Features

• No need for the preparation of rat and mouse models that are otherwise time-consuming.
• Enables stable dissociation and culture of neurons by combining with neuron culture medium and neuron dissociation solution.

Protocol

Dispersion and Isolation of Frozen Neurons

The products in our frozen neuron series were thawed, and our dissociation solution was used to disperse and isolate the cells. Cell viability was calculated as % of total cells.

• 

• 	Product name
  ①	Hippocampus, from mouse (embryonic day 16)	Total number of cells(cells/vial)	4.32 x 105
  		Total number of viable cells(cells/vial)	3.83 x 105
  		Cell viability(%)	89
  ②	Cerebral cortex, from mouse (embryonic day 15)	Total number of cells(cells/vial)	6.64 x 106
  		Total number of viable cells(cells/vial)	6.09 x 106
  		Cell viability(%)	92
  ③	Hippocampus, from rat (embryonic day 19)	Total number of cells(cells/vial)	1.37 x 106
  		Total number of viable cells(cells/vial)	1.24 x 106
  		Cell viability(%)	90
  ④	Cerebral cortex, from rat (embryonic day 17)	Total number of cells(cells/vial)	1.32 x 107
  		Total number of viable cells(cells/vial)	1.17 x 107
  		Cell viability(%)	89
  ⑤	Cerebral striatum, from rat (embryonic day 17)	Total number of cells(cells/vial)	2.73 x 106
  		Total number of viable cells(cells/vial)	2.58 x 106
  		Cell viability(%)	95

The data demonstrated that neurons were isolated from frozen samples with approximately 90% viability.

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