Wako 神经元解离解决方案Neuron Dissociation Solutions

For simple isolation of neurons from brain tissues

Neuron Dissociation Solutions

Our neuron dissociation solutions can be used to dissociate and isolate neurons from tissues derived from the central nervous system of rat and mouse. It is composed of 3 solutions (enzyme, dissociation, and isolation solutions).
The product is ready-to-use without the need for preparations, and achieves simple isolation of neurons while maintaining high cell viability.

用于从脑组织中简单分离神经元

神经元解离解决方案

我们的神经元分离解决方案可用于从大鼠和小鼠中枢神经系统的组织中分离和分离神经元。它由 3 种溶液（酶、解离和分离溶液）组成。
该产品即用即用，无需制备，在保持高细胞活力的同时实现了神经元的简单分离。

Features
Protocol
Dissociation and Isolation of Frozen Neurons
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Features

Simple and stable isolation of neurons
Ready to Use

Protocol

Reagents needed

Frozen neuron series
Neuron dissociation solutions (code No. 291-78001) or neuron dissociation solutions S (code No. 297-78101)
Neuron culture medium (code No. 148-09671)

When using frozen neurons

4. Washing solution: Leibovitz’s L-15 medium (code No. 128-06075) or HPSS (+) (code No.128-06075)

所需试剂

冷冻神经元系列
神经元解离溶液（代码编号 291-78001）或神经元解离溶液 S（代码编号 297-78101）
神经元培养基（编号148-09671）

使用冷冻神经元时

　4、洗涤液：Leibovitz’s L-15培养基（代号128-06075）或HPSS（+）（代号128-06075）

Experimental protocol

Experimental condition	Type of solution	Amount	Recommended products
For samples containing large tissues that need to be dissociated (1-3 cerebral hemispheres from mouse or rat embryos)	Enzyme solution	5.0mL	Neuron Dissociation Solutions (Code: 291-78001)
Dispersion solution	3.0mL
Isolation solution	5.0mL
For samples containing small tissues that need to be dissociated (1-8 hippocampi from mouse or rat embryos)	Enzyme solution	2.5mL	Neuron Dissociation Solutions S (Code: 297-78101)
Dispersion solution	2.5mL
Isolation solution	2.5mL

Dissociation and Isolation of Frozen Neurons

Frozen neurons from our company were thawed, and were dissociated and isolated in the neuron dissociation solutions.

	Product Name
①	Hippocampus, from mouse (embryonic day 16)	Total(cells/vial)	4.32 x 10⁵
		Viable(cells/vial)	3.83 x 10⁵
		Cell viability(%)	89
②	Cerebral cortex, from mouse (embryonic day 15)	Total(cells/vial)	6.64 x 10⁶
		Viable(cells/vial)	6.09 x 10⁶
		Cell viability(%)	92
③	Hippocampus, from rat (embryonic day 19)	Total(cells/vial)	1.37 x 10⁶
		Viable(cells/vial)	1.24 x 10⁶
		Cell viability(%)	90
④	Cerebral cortex, from rat (embryonic day 17)	Total(cells/vial)	1.32 x 10⁷
		Vialble(cells/vial)	1.17 x 10⁷
		Cell viability(%)	89
⑤	Cerebral striatum, from rat (embryonic day 17)	Total(cells/vial)	2.73 x 10⁶
		Viable(cells/vial)	2.58 x 10⁶
		Cell viability(%)	95

▶The data demonstrated that the use of neuron dissociation solutions enables isolation of cells from frozen samples with high viability (~90%).

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