Wako 神经元解离解决方案Neuron Dissociation Solutions
For simple isolation of neurons from brain tissues
Neuron Dissociation Solutions
Our neuron dissociation solutions can be used to dissociate and isolate neurons from tissues derived from the central nervous system of rat and mouse. It is composed of 3 solutions (enzyme, dissociation, and isolation solutions).
The product is ready-to-use without the need for preparations, and achieves simple isolation of neurons while maintaining high cell viability.
神经元解离解决方案
我们的神经元分离解决方案可用于从大鼠和小鼠中枢神经系统的组织中分离和分离神经元。它由 3 种溶液(酶、解离和分离溶液)组成。
该产品即用即用,无需制备,在保持高细胞活力的同时实现了神经元的简单分离。
- Features
- Protocol
- Dissociation and Isolation of Frozen Neurons
- Product List
- Related Product List
- Related Information
Features
- Simple and stable isolation of neurons
- Ready to Use
Protocol
Reagents needed
- Frozen neuron series
- Neuron dissociation solutions (code No. 291-78001) or neuron dissociation solutions S (code No. 297-78101)
- Neuron culture medium (code No. 148-09671)
When using frozen neurons
4. Washing solution: Leibovitz’s L-15 medium (code No. 128-06075) or HPSS (+) (code No.128-06075)
所需试剂
- 冷冻神经元系列
- 神经元解离溶液(代码编号 291-78001)或神经元解离溶液 S(代码编号 297-78101)
- 神经元培养基(编号148-09671)
使用冷冻神经元时
4、洗涤液:Leibovitz’s L-15培养基(代号128-06075)或HPSS(+)(代号128-06075)
Experimental protocol

Recommended volumes of dissociation solutions
Experimental condition | Type of solution | Amount | Recommended products |
---|---|---|---|
For samples containing large tissues that need to be dissociated (1-3 cerebral hemispheres from mouse or rat embryos) |
Enzyme solution | 5.0mL | Neuron Dissociation Solutions (Code: 291-78001) |
Dispersion solution | 3.0mL | ||
Isolation solution | 5.0mL | ||
For samples containing small tissues that need to be dissociated (1-8 hippocampi from mouse or rat embryos) |
Enzyme solution | 2.5mL | Neuron Dissociation Solutions S (Code: 297-78101) |
Dispersion solution | 2.5mL | ||
Isolation solution | 2.5mL |
Dissociation and Isolation of Frozen Neurons
Frozen neurons from our company were thawed, and were dissociated and isolated in the neuron dissociation solutions.
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Product Name ① Hippocampus, from mouse (embryonic day 16) Total(cells/vial) 4.32 x 105 Viable(cells/vial) 3.83 x 105 Cell viability(%) 89 ② Cerebral cortex, from mouse (embryonic day 15) Total(cells/vial) 6.64 x 106 Viable(cells/vial) 6.09 x 106 Cell viability(%) 92 ③ Hippocampus, from rat (embryonic day 19) Total(cells/vial) 1.37 x 106 Viable(cells/vial) 1.24 x 106 Cell viability(%) 90 ④ Cerebral cortex, from rat (embryonic day 17) Total(cells/vial) 1.32 x 107 Vialble(cells/vial) 1.17 x 107 Cell viability(%) 89 ⑤ Cerebral striatum, from rat (embryonic day 17) Total(cells/vial) 2.73 x 106 Viable(cells/vial) 2.58 x 106 Cell viability(%) 95
▶The data demonstrated that the use of neuron dissociation solutions enables isolation of cells from frozen samples with high viability (~90%).
Product List
Neuron Dissociation Solutions
for Cell Culture
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -80 degrees C.
Comparison
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Product Number
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Package Size
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Price
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Inventory
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4Set
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In stock in Japan |
for Cell Culture
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -80 degrees C.
Comparison
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Product Number
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Package Size
|
Price
|
Inventory
|
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10Set
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In stock in Japan |
Related Product List
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Culture medium
Frozen nerve cells
Cryopreservation solution
Basal Medium / supplement
Related Information
Category
- Cell Culture
- Nerve Cell Culture
- Cell Dispersion / Detachment
- Life Science
- Neuroscience
- Neural Cell Culture
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