Wako和光纯药 Lysyl Endopeptidase 赖氨酰内肽酶，质谱级
Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)
Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase®. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.
Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C) is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.
赖氨酰内肽酶®，质谱级 (Lys-C)
蛋白质组分析中最重要的技术之一是凝胶内消化蛋白质斑点/条带，这些蛋白质斑点/条带已使用消化酶（如胰蛋白酶和赖氨酰内肽酶® ）通过电泳分离。蛋白质可以通过凝胶内消化产生的肽的质谱分析来识别，并且可以获得关于翻译后修饰的进一步信息。
Lysyl Endopeptidase ® , 质谱级 (Lys-C) 是一种冷冻干燥的产品，保留了足够的凝胶内消化活性，并以非常小的数量包装以方便使用。
- Recombinant
Lysyl Endopeptidase® - Biochemistry Grade
Lysyl Endopeptidase®
- Features
- Application Data
- References
- Product List
- Related Product List
- Related Information
Features
- High specificity and efficiency of protein digestion allow for easy database searches by peptide mass.
- Improved cleavage at lysine residue and increase in the number of peptides are obtained by combination with trypsin.
- Packed in very small quantities according to the amounts used so that sufficient activity for in-gel digestion may be retained.
Application Data
Comparison of In-gel Digestion Using Trypsin (Tp), Lysyl Endopeptidase® (Lep) and Lep Combined with Tp (Lep +Tp)
BSA band (100ng) resolved by SDS-PAGE was in-gel digested with Tp, Lep and Lep +Tp and analyzed by MALDI-TOFMS. The figure shows the individual mass spectra. The evaluation of these peptidases is summarized in the table.
使用胰蛋白酶 (Tp)、赖氨酰内肽酶® (Lep) 和 Lep 结合 Tp (Lep +Tp)进行凝胶内消化的比较
通过 SDS-PAGE 解析的 BSA 条带 (100ng) 用 Tp、Lep 和 Lep +Tp 进行凝胶内消化，并通过 MALDI-TOFMS 进行分析。该图显示了单独的质谱图。这些肽酶的评价总结在表中。
Table: Comparison of Trypsin (Tp), Lysyl Endopeptidase® (Lep) and Lep + Tp
| Tp | Lep | Tp + Lep | |
|---|---|---|---|
| Cleavage site | C terminal of Arg + Lys | C terminal of Lys | C terminal of Arg + Lys |
| Missed cleavage (Rates of missed cleavage)※ | Many (8%) | Very few (0%) | Few (3%) |
| No. of identified peptides | 17 | 19 | 22 |
Data provided by: Wada, Y., Osaka Women’s and Children’s Hospital
These results indicate there are very few missed cleavages obtained by Lep digestion. When Tp is used concomitantly with Lep, missed cleavages decrease and the number of identified peptides increase compared to when only Tp is used.
*The value resulted from subtracting the coverage obtained when database searches were performed with Missed cleavage 0 from that obtained when performed with Missed cleavage 1. “Coverage” is the percentage of peptides obtained after in-gel digestion in the whole sequence.
Mass spectrum: Comparison of Trypsin (Tp), Lysyl Endopeptidase® (Lep) + Tp
References 参考文献
- Wada, Y. and Kadoya, M.: J. Mass Spectrom., 38, 117(2003).
- Shevchenko, A., Wilm, M., Vorm, O. and Mann., M.: Anal. Chem., 68, 850(1996).
Product List
Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)
for Proteome Research
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
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Comparison
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20ug x5
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In stock in Japan |
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20ug
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In stock in Japan |
Trypsin, Mass Spectrometry Grade
Trypsin, from Porcine Pancreas, Mass Spectrometry Grade
for Proteome Research
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
- GHS :
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Comparison
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Product Number
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Package Size
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Price
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Inventory
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20ug x5
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In stock in Japan |
Related Product List
Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)
for Cell Culture
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
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Comparison
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Product Number
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Package Size
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Price
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Inventory
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20ug
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Inquire |
Lysyl Endopeptidase®, Biochemistry Grade (Lys-C)
Lysyl EndopeptidaseR (Lys-C)
for Biochemistry
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
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Comparison
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Product Number
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Package Size
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Price
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Inventory
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1vial(10AU)
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In stock in Japan |
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1vial(2AU)
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In stock in Japan |
Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)
Lysyl Endopeptidase®, Biochemistry Grade (Lys-C)
Related Information
Category
- Life Science
- Proteomics
- Enzyme
- Life Science
- Enzyme
- Protease
- Cell Culture
- Biopharmaceuticals Manufacturing
- Quality Control Test
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