Wako和光纯药 Lysyl Endopeptidase 赖氨酰内肽酶
Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)
This product is a recombinant Lysyl Endopeptidase® expressed in E. coli. Lysyl endopeptidase® is a serine protease that cleaves peptide bonds at the carboxy-terminus of lysine residues with high specificity.
Taking advantage of its excellent specificity, Lysyl Endopeptidase® is used for peptide fragmentation and peptide mapping for analysis of the primary structure of proteins. This product is checked residual DNA and Host cell protein for biopharmaceutical analysis.
赖氨酰内肽酶®，重组，生物药物分析级 (rLys-C)
该产品是在大肠杆菌中表达的重组赖氨酰内肽酶®。赖氨酰内肽酶®是一种丝氨酸蛋白酶，可高度特异性地切割赖氨酸残基羧基末端的肽键。Lysyl Endopeptidase ®利用其出色的特异性，可用于肽片段化和肽图分析，以分析蛋白质的一级结构。本产品经检查残留 DNA 和宿主细胞蛋白，用于生物制药分析。
- Brochure
- Biochemistry Grade
Lysyl Endopeptidase® - Mass Spectrometry Grade
Lysyl Endopeptidase®
- Features
- Specification
- BSA Digestion Analysis
- Procedure
- Product List
- Related Product List
- Related Information
Features
- Expressed in E. coli
- Checked residual DNA / Host cell protein
- Missed cleavage rate is lower than competitor‘s Lys-C
Specification
| Requirement | Specification |
|---|---|
| Appearance | Lyophilisate |
| Activity | ≧2.0AU/mg |
| DNA residual test | ≦10ng/mg |
| HCP assay | ≦1.0μg/mg |
| Endotoxin testing | ＜20EU/mg |
| Electrophoresis test (SDS-PAGE) | to pass test |
规格
| 要求 | 规格 |
|---|---|
| 外貌 | 冻干物 |
| 活动 | ≧2.0AU/mg |
| DNA残留检测 | ≤10ng/mg |
| HCP测定 | ≤1.0μg/mg |
| 内毒素检测 | ＜20EU/mg |
| 电泳测试（SDS-PAGE） | 通过测试 |
BSA Digestion Analysis
We incubated BSA with each protease for 1 hour and 18 hours.
After incubations, we analized the missed cleavage rate and number of peptides.
| Our Product | Competitor’s Product | |
|---|---|---|
| Specificity (Missed cleavage rate after 1 hour’s incubation) |
0% | 0% |
| Specificity (Missed cleavage rate after 18 hours’ incubation) |
10% | 20% |
| Activity (Number of peptides after 18 hours’ incubation) |
41 | 35 |
➡ The data suggested that our rLys-C have higher activity and specificity in long time reaction than the competitor’s product.
Procedure
1. Sample preparation
①Dissolve or dilute the protein sample to be digested with 25 mmol/L Tris-HCl, 1 mmol/L EDTA，pH 8.5~9.0.
②Add Disulfide threitositol (DTT) or β-mercaptoethanol to the solubilized protein at the final concentration of 5 mmol/L.
③Incubate for 30 min at room temperature.
④Add iodoacetamide to the solubilized protein at the final concentration of 10 mmol/L.
⑤Incubate in dark for 30 min at room temperature.
2. rLys-C preparation
①Dissolve lyophilized powder of rLys-C with 100 μl 12.5 mmol/L Tris-HCl, pH 8.5~9.0.
3. Sample digestion
①Add the prepared rLys-C to sample solution according to the enzyme : protein mass ratio of 1:20~1:100, and incubate at 35℃~37℃ for 2~18 hours.
②The final concentration of 0.5~1.0v/v% trifluoroacetic acid is added to stop the reaction.
程序
1. 样品制备
①用25 mmol/L Tris-HCl，1 mmol/L EDTA，pH 8.5~9.0溶解或稀释待消化的蛋白质样品。
②在溶解的蛋白质中加入二硫化物苏糖醇（DTT）或β-巯基乙醇至终浓度为5 mmol/L。
③室温孵育30分钟。
④在溶解的蛋白质中加入碘乙酰胺，终浓度为10 mmol/L。
⑤室温避光孵育30分钟。
2. rLys-C 的制备
①用100 μl 12.5 mmol/L Tris-HCl，pH 8.5~9.0溶解rLys-C冻干粉。
3. 样品消解
①将配制好的rLys-C按酶：蛋白质量比1:20~1:100加入样品溶液，35℃~37℃孵育2~18小时。
②加入终浓度0.5~1.0v/v%的三氟乙酸终止反应。
Product List
Lysyl Endopeptidase®, recombinant, Biopharmaceutical Analysis Grade (rLys-C)
for Cell Culture
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
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Comparison
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Product Number
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Package Size
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Price
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Inventory
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|---|---|---|---|---|
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20ug
|
Related Product List
Biochemistry Grade
Lysyl EndopeptidaseR (Lys-C)
for Biochemistry
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
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Comparison
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Product Number
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Package Size
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Price
|
Inventory
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|---|---|---|---|---|
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1vial(10AU)
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In stock in Japan |
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1vial(2AU)
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In stock in Japan |
Mass spectrometry grade
Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C)
for Proteome Research
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at -20 degrees C.
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Comparison
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Product Number
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Package Size
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Price
|
Inventory
|
|---|---|---|---|---|
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20ug x5
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In stock in Japan |
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20ug
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|
In stock in Japan |
Biochemistry Grade
Mass spectrometry grade
Related Information
Category
- Life Science
- Enzyme
- Protease
- Cell Culture
- Biopharmaceuticals Manufacturing
- Quality Control Test
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