Wako和光纯药 昆虫细胞培养基Insect Cell Culture Medium: WakoVAC PSFM-J1
Insect Cell Culture Medium: WakoVAC PSFM-J1
Licensed Formulation
Formulated for insect cell expansion. Designed to maximize insect cell growth, viability and productivity. WakoVAC PSFM-J1 manufactured in Wako outperforms the same formulation manufactured in competitor’s site due to using quality raw materials and strict and robust raw material control.
Raw Material
All raw materials are documented and traceable. All raw materials used in WakoVAC PSFM-J1 are standardized to a certain specification, scalable, and provided from validated suppliers. WakoVAC PSFM-J1 contains fish-derived component (cGMP, virus-inactivated) and Hydorlysate. Without fish-derived component, the media can be defined animal component-free.
Industrial Applications
The intended use of WakoVAC (GMP-compliant manufactured) is for research, process development, large-scale manufacturing/commercialization. WakoVAC is scalable to commercial volumes for industrial application. Both liquid and powder are available. Flexible packaging is also available upon request. Wako provides complete workflow support from media optimization service to regulatory support at the time of IND/NDA.
Performance
WakoVAC PSJF-J1 supports a wide range of insect cells: Sf+, Hi5, Sf9, Sf21. It is designed to maximize and consistent insect cell growth, viability and sustains high productivity. Best in class performance, Easy to use, Ongoing stability.
许可配方
专为昆虫细胞扩增而配制。旨在最大限度地提高昆虫细胞的生长、活力和生产力。由于使用优质原材料和严格而稳健的原材料控制，Wako 制造的 WakoVAC PSFM-J1 的性能优于竞争对手现场制造的相同配方。
原材料
所有原材料都有记录和可追溯。WakoVAC PSFM-J1 中使用的所有原材料均已标准化为特定规格、可扩展，并由经过验证的供应商提供。WakoVAC PSFM-J1 含有鱼源成分（cGMP，病毒灭活）和水解物。没有鱼源成分，培养基可以定义为不含动物成分。
工业应用
WakoVAC（符合 GMP 制造）的预期用途是用于研究、工艺开发、大规模制造/商业化。WakoVAC 可扩展到工业应用的商业量。液体和粉末都可用。也可应要求提供软包装。Wako 在 IND/NDA 时提供从媒体优化服务到监管支持的完整工作流程支持。
表现
WakoVAC PSJF-J1 支持多种昆虫细胞：Sf+、Hi5、Sf9、Sf21。它旨在最大限度地和一致的昆虫细胞生长、活力和维持高生产力。一流的性能，易于使用，持续的稳定性。
- Brochure
- Features
- Culturing Sf9 Cells
- Culturing Hi5 Cells
- Target expression data with Sf21
- Product List
- Related Product List
- Related Information
Features
- Support wide range of insect cells
- Performs best with Sf+
- Cell Growth
- Cell Viability
- Cell Growth
- Fish-derived component(cGMP) and hydrolysate contained
- CoA for non-GMP and GMP available
Cell growth, cell viability, and protein expression data can be included on CoA upon request - Intended use: for research and further manufacturing
| Wako Cat# 160-25851 | TBD | |
|---|---|---|
| Format | Liquid | Powder |
| Package | 1L | 8kg and/or Customs upon request |
| Shelf-life | 1 year from the date of MFG | 2 years from the date of MFG |
| Storage Condition | 2-10˚C, protect from light | |
Other Information
- Assure lot-to-lot consistency
- Fine protein expression as well with Sf9, Sf21 and Hi5
- L-Glutamine does not need to be supplemented
- Comparative Landscape
Product Description Supplier SF ACF PF CD GMP Hydrolysate Cell Type Shelf-life Storage Size Bag Avaiability Sf Insect FUJIFILM Irvine Scientific Yes Yes Yes + Sf9, Sf21 1 year 2-8℃ 1L 5L(MTO) Custom upon request WakoVAC PSFM-J1 FUJIFILM Wako Yes Fish-derived component Yes Yes ++ Sf+, Hi5, Sf9, and Sf21 1 year 2-10℃ 1L 10L and 20L Powder available
Culturing Sf9 Cells
Retain robust cell growth, viability and target expression with Sf9
Cell Growth |
Protein Expression Testing
* Data provided by the Goshima Lab from Nagoya University |
|
Cell Viability |
Culturing Hi5 Cells
Retain robust cell growth, viability and target expression with Hi5
Cell Growth |
Protein Expression Testing
* Data provided by Mirai-Kougaku |
|
Cell Viability |
Target expression data with Sf21
Retain target expression with Sf21
Protein Expression Testing
| M: Molecular weight size marker Lane 1: Medium A (Pre-viral inoculation) Lane 2: Medium A (Post-viral inoculation) Lane 3: PSFM-J1 (Pre-viral inoculation) Lane 4: PSFM-J1 (Post-viral inoculation) ←: Target protein |
- The amount of samples being used: 15μl/Lane
- SDS-PAGE: 5-20% gradient gel, 200v constant
- Voltage for 50 minutes
* Data provided by the Goshima Lab from Nagoya University
Product List
-
PSFM-J1 Medium Wako, Liquid
for Cell Culture
- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at 2-10 degrees C.
ComparisonProduct NumberPackage SizePriceInventory- 160-25851
1L- List Price
- JPY 7,300
In stock in Japan
- 162-25855
500mLDiscontinued
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Price
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Inventory
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|---|---|---|---|---|
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10EA
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In stock in Japan |
SuperSep (TM) Ace, 10-20%, 17well
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- Manufacturer : FUJIFILM Wako Pure Chemical Corporation
- Storage Condition : Keep at 2-10 degrees C.
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Related Information
Category
- Cell Culture
- Insect Cell Culture
- Medium
- Cell Culture
- Vaccine Manufacturing
- Medium
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