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New 8-OHdG Check

Suitable for assessment of oxidative stress using urine samples. For research use only.

About 8-hydroxy-2′-deoxyguanosine (8-OHdG):

New 8-OHdG Check

8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.

New 8-OHdG Check

This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells, we recommend to use ‘Highly Sensitive 8-OHdG Check ELISA’.

Content of this kit

8-OHdG Microtiter Plate:	Precoated with 8-OHdG(12 X 8wells, split type)	1 plate
Primary Antibody:	Anti 8-OHdG antibody, powder.	1 vial
Primary Antibody Solution		1 vial (6mL)
Secondary Antibody:	HRP-anti mouse antibody, powder.	1 vial
Secondary Antibody Solution:		1 vial (12mL)
Chromatic Solution:	3,3′,5,5′-tetramethylbenzidine	1 vial (0.25mL)
Diluting Solution:	H2O2 containing buffer.	1 vial (12mL)
Washing Solution(5x):		2 vials (26mL x 2)
Reaction Terminating Solution:	1M Phosphoric acid.	1 vial (12mL)
Standard 8-OHdG Solution:	Purified 8-OHdG (0.5, 2, 8, 20, 80, 200 ng/mL)	1 vial each
Plate Seal:		2 sheets

Specifications

Assay principle:	Competitive ELISA (detection: 450 nm)
Specifity:	Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Measuring range:	0.5 to 200 ng/mL
Format:	96 wells (18 samples in triple assays)
Applications:	Urine from human and animals. For serum and tissue samples, Highly Sensitive 8-OHdG Check (code #KOG-HS10E) is recommended.
Storage:	Store at 4 – 10°C (don’t freeze).
Expiry:	12 months after the day of manufacturing.
Required but not provided:	Micropipet and chip (100 micro L, 1000 micro L). Measuring pipet (10 mL, 20 mL)/ measuring cylinders. 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. Microplate reader (filter; 450 nm).

References

	1)	H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986) [ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ]
	2)	R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991) [ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ]
	3)	S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2’deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997) [ Specifity of the antibody clone N45.1.]
	4)	Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000) [ Development and assessment of 8-OHdG Check ELISA. ]
	5)	Nagayoshi Y, Kawano H, Hokamaki J, Miyamoto S, Kojima S, Shimomura H, Tsujita K, Sakamoto T, Yoshimura M, Ogawa H: Urinary 8-hydroxy-2′-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events. Am J Cardiol. 95(4), p514-517 (2005) [ Measurement of 8-OHdG in human urine. ]
	6)	Morillas-Ruiz J, Zafrilla P, Almar M, Cuevas MJ, Lopez FJ, Abellan P, Villegas JA, Gonzalez-Gallego J: The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists. Eur J Appl Physiol 95(5-6),p543-549(2005) [ Study about exercise, antioxidant supplements and urinary 8-OHdG. ]
	7)	Someya T, Kaneko K, Yamada T, Yamashiro Y: Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats. Pediatr Nephrol 20(10),p1430-1434(2005) [ Effect of antioxidants to 8-OHdG in rat urine. ]
	8)	Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci 250(1-2),p159-161(2006) [ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ]

[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.

Product name	Code	Assay range	Format	Application
New 8-OHdG Check	KOG-200SE	0.5-200 ng/mL	96 wells	Urine and animal serum.