日本老化Jaica-New 8-OHdG Check

Products > New 8-OHdG Check ELISA

Suitable for assessment of oxidative stress using urine samples. For research use only.

About 8-hydroxy-2′-deoxyguanosine (8-OHdG):

8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterials from human and animals.

This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. New 8-OHdG Check ELISA is suitable for urine and serum sample from animal and human. If you are planning to measure 8-OHdG in human serum,tissue, cultured cells, we recommend to use ‘Highly Sensitive 8-OHdG Check ELISA’.

Content of this kit

Specifications

References

[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for assessment of oxidative stress in serum, tissue and cultured cells. For research use only.

About 8-hydroxy-2′-deoxyguanosine (8-OHdG):

8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. New 8-OHdG Check is a competitive enzyme-linked immunosorbent assay (ELISA) utilising monoclonal antibody (clone N45.1) which is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. This product is suitable for detection of 8-OHdG in urine and other biomaterialsfrom human and animals. This product is a 8-OHdG ELISA kit utilizing anti 8-OHdG monoclonal antibody (clone N45.1) which is highly specific for 8-OHdG. We provide two types of 8-OHdG ELISA kits with different assay range. Highly Sensitive 8-OHdG Check ELISA is suitable for urine, serum , tissue and cultured cells.

Specifications

Assay principle: Competitive ELISA (detection: 450 nm) Specifity: Specific for 8-OHdG. Antibody have been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G). Measuring range: 0.125 to 10 ng/mL Format: 96 wells (18 samples in triple assays) Applications: Urine, serum, tissue and cultured cells. Storage: Store at 4 – 10°C (don’t freeze). Expiry: 9 months after the day of manufacturing. Required but not provided: Micropipet and chip (100 µL, 1000 µL). Measuring pipet (10 mL, 20 mL)/ measuring cylinders. 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. Microplate reader (filter; 450 nm).

Content of this kit

8-OHdG Microtiter Plate: Precoated with 8-OHdG(12 X 8wells, split type) 1 plate Primary Antibody: Anti 8-OHdG antibody, powder. 1 vial Primary Antibody Solution   1 vial (6mL) Secondary Antibody: HRP-anti mouse antibody, powder. 1 vial Secondary Antibody Solution:   1 vial (12mL) Chromatic Solution: 3,3′,5,5′-tetramethylbenzidine 1 vial (0.25mL) Diluting Solution: H2O2 containing buffer. 1 vial (12mL) Washing Solution(5x):   2 vials (26mLx2) Reaction Terminating Solution: 1M Phosphoric acid. 1 vial (12mL) Standard 8-OHdG Solution: Purified 8-OHdG (0.125, 0.25, 0.5, 1, 4, 10 ng/mL) 1 vial each Plate Seal:   2 sheets

References

1) H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura,A.Ootsuyama and H.Tanooka : Formation of 8-hydroxyguaine moiety in cellular DNA by agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7(11), p1849-1851 (1986) [ 8-OHdG/8-OHG is formed by reactive oxygen radicals. ] 2) R.G.Cutler: Antioxidants and aging. Am J Clin Nutr 50,p373S-379S (1991) [ Relationship between lifespan and antioxidants, enzymes and 8-OHdG. ] 3) S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2’deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab Invest 76(3), p365-374 (1997) [ Specifity of the antibody clone N45.1.] 4) Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K: Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol 107(1-2),p39-44 (2000) [ Development and assessment of 8-OHdG Check ELISA. ] 5) M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert and J.Lunec: Discrepancies in the measurement of UVC-induced 8-oxo-2′-deoxyguanosine: Implications for the analysis of oxidative DNA damage. Biochem Biophys Res Commun 259, p374-378 (1999) [ ELISA results show high correlation between those of HPLC-ECD when 8-OHdG in DNA samples are assessed. ] 6) Ha Won Kim, Akira Murakami, Marshall V Williams and Hajime Ohigashi: Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells. Carcinogenesis 24(2), p235-241 (2003) [ Detection of 8-OHdG in cultured cells. ] 7) Watanabe E, Matsuda N, Shiga T, Kajimoto K, Ajiro Y, Kawarai H, Kasanuki H, Kawana M: Significance of 8-hydroxy-2′-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy. J Card Fail. 2006 Sep;12(7):527-32. [ Serum 8-OHdG in IDC patients are assessed. ] 8) Shiihara T, Kato M, Ichiyama T, Takahashi Y, Tanuma N, Miyata R, Hayasaka K: Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage. J Neurol Sci. 250(1-2):159-61(2006) [ Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF). ] 9) Reiko Nagasaka, Nobuaki Okamoto, Hideki Ushio: Effects of caloric restriction on post-spawning death of ayu. Exp Gerontol 40, p556-561(2005) [ Detection of 8-OHdG in fish tissues (brain and liver). ] 10) Yasuda M, Ide H, Furuya K, Yoshii T, Nishio K, Saito K, Isotani S, Kamiyama Y, Muto S, Horie S: Salivary 8-OHdG: a useful biomarker for predicting severe ED and hypogonadism. J Sex Med. 5(6),p1482-1491(2008) [ 8-OHdG in saliva can be detected by 8-OHdG ELISA. ]

Product name Code Assay range Application Highly Sensitive 8-OHdG Check KOG-HS10E 0.125 – 10 ng/mL Serum, plasma, tissue and other biological fluids. Made in Japan.

Related products:&nbsp&nbsp&nbsp New 8-OHdG Check ELISA  &nbsp Anti 8-OHdG monoclonal antibody

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual Quality control Technical information   Application to serum Application to tissue and cultured cells

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry and ELISA. For research use only.

About 8-hydroxy-2′-deoxyguanosine (8-OHdG):

Reactive oxygen species (ROS) and oxidative stress may be crucial for development of various diseases such as diabetes, cancer, and may play an important role in the aging process. 8-hydroxy-2′-deoxyguanosine (8-OHdG) is a product of oxidatively damaged DNA formed by hydroxy radical, singlet oxygen and direct photodynamic action. 8-OHdG can be detected in tissue, serum, urine and other biomaterials. Anti 8-OHdG monoclonal antibody (clone N45.1) is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine. Suitable for immunohistochemistry and ELISA. Epidermis from hairless mouse by chronic UVB irradiation after 4 weeks of treatment stained with N45.1. (Y.Hattori, et.al.: J Invest Dermatol 107, p733-737, 1996)

Specifications

Clone #: N45.1 Antigen: 8-OHdG-conjugated Keyhole Limpet Hemocyanin Subclass: Mouse IgG1(kappa) Prepared as ascite, and ammnonium sulphate purified. Form: Lyophilized Powder Package: 20 or 100 µg of IgG / vial (MOG-020P and MOG-100P respectively). Specificity: 19 analogues of 8-OHdG {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%). Use: Immunohistochemistry and ELISA Storage: Less than -20°C for 5 years When transport, stable at least 7 days at room temperature.

References

1) S.Toyokuni, T.Tanaka, Y.Hattori, Y,Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa: Quantitative immunohistochemical determination of 8-hydroxy-2’deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab. Invest. 76(3), p365-374 (1997) [ Specifity of the antibody clone N45.1.] 2) Y.Hattori, C.Nishigori, T.Tanaka, K.Uchida, O.Nikaido, T.Osawa, H.Hiai, S.Imamura, and S.Toyokuni: 8-Hydroxy-2’deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure. J. Invest. Dermatol. 107, p733-737 (1996) [ Immunohistochemistry of UV-B irradiated mouse skin. ] 3) T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni: Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997) [ Immunohistochemistry of ferric nitrotriacetate-treated mouse kidney. ] 4) Y.Ihara, S.Toyokuni, K.Uchida, H.Odaka, T.Tanaka, H.Ikeda, H.Hiai, Y.Seino, and Y.Yamada: Hyperglycemia causes oxidative stress in pancreatic -cells of GK rats, a model of type 2 diabetes. Diabetes 48, p927-932 (1999) [ Immunohistochemistry of pancreatic islets from GK rat, a diabetes model. ] 5) T. Ichiseki, A. Kaneuji, S. Katsuda1, Y. Ueda,T. Sugimori and T. Matsumoto: DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis. Rheumatology 44,p456-460(2005) [ Immunohistochemistry of rabbit tissue. ] 6) Lee YA, Cho EJ, Yokozawa T: Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under H2O2 -induced cellular senescence. Biol Pharm Bull 31(6)p1265-1269(2008) [ Application to human cultured cells. ]

Product name Code Content Anti 8-OHdG monoclonal antibody MOG-020P 20 µg of IgG MOG-100P 100 µg of IgG Made in Japan.

Related products:&nbsp&nbsp&nbsp New 8-OHdG Check ELISA  &nbsp Highly sensitive 8-OHdG Check ELISA

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual Immunohistochemical procedure Technical Information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry. For research use only.

[ Specific to DNA oxidation ]

Thymidineglycol (TG) is one of the major oxidation products of DNA. Thymidine (T) can be damaged by oxidative stress such as radiation and energy metabolism. Two different pathways to form TG have been suggested. Deoxythymidine in DNA is directly oxidised by hydroxy radical, to form TG. TG can be also formed through an intermediate thymidine chlorohydrin, which is derived from hypochlorous acid (HOCl) from neutrophil myeloperoxidase. Thymidineglycol is derived from DNA, not from RNA. TG is the oxidative stress marker secific for DNA damage.

[ Comparison with 8-OHdG ]

8-hydroxy-2′-deoxyguanosine (8-OHdG) is one of the well-known DNA oxidation products. In an lipopolysaccharide (LPS)-treated mouse liver, 8-OHdG can be stained in 24 hours after LPS treatment. TG can be detected in 6 hours, and remain at least for 72 hours. Anti TG monoclonal antibody (clone 2E8) has been established by Dr. Toshihiko Osawa (Nagoya Univ.). Poly dT (50 mer) has been oxidised by OsO4 and injected to Balb/c mouse as immunogen. This antibody can react with thymidineglycol in DNA or TG polymer, and suitable for immunohistochemistry.

[ Specifications ]

Antigen: Thymidineglycol polymer. Form: Lyophilized power. Reconstitute by 1000 µL of distilled water. 100 µg/mL of IgG. Subclass: Mouse IgG1 (kappa), clone 2E8 Specificity: Specific for DNA fragment containing thymidine glycol, and thymidine glycol polymer. Cross reactivity have been tested to following analogues: oxidized deoxycytidine polymer, oxidized deoxyguanosine polymer and oxidized deoxyadenosine polymer. Not react with free thymidineglycol. Application: Immunohistochemistry (antibody conc. 5 – 10 µg/mL) Storage: Less than -20°C

Reference

1) Ito K, Yano T, Morodomi Y, Yoshida T, Kohno M, Haro A, Shikada Y, Okamoto T, Maruyama R, Maehara Y. Serum antioxidant capacity and oxidative injury to pulmonary DNA in never-smokers with primary lung cancer. Anticancer Res. 32(3),p1063-1067(2012)

Product name Code Content Anti thymidineglycol (TG) monoclonal antibody MTG-100P 100 µg IgG

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual (PDF) Technical Information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.

What is HEXANOYL-LYSINE ?

Biomarker for early stage of lipid oxidation Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids. Hexanoyl-Lys (HEL) ELISA kit JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.

Specifications

Assay principle: Competitive ELISA (detection: 450 nm) Specifity: Specific to N-epsilon-Hexanoyl-Lysine adduct Measuring range: 2 – 700 nmol/L Time to answer: Over night and 2 hours. Format: 96 wells (54 samples in single assay) Applications: Urine, serum and cultured cells from human and animals. Storage: Store at 2 – 8°C (don’t freeze). Expiry: 2 years after the day of manufacturing. Required but not provided: 50 µL micropipettor and pipette tips 8-channel (50-200 µL) micropipettor and tips 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. 4-7 °C incubator Microtiter plate reader (measuring wavelength 450 nm)

Content of this kit

HEL-coated Microtiter Plate: 1 plate (96 wells) Primary Antibody (ready to use): 1 vial Secondary Antibody: 1 vial Secondary Antibody Buffer: 1 vial Chromogen (TMBZ solution): 1 vial Chromogen Buffer: 1 vial Washing Buffer (5X): 1 vial Stop Solution: 1 vial Standard solution (6 levels): 1 vial each Plate seal: 2 sheets

References

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999) Identification of HEL, which is lysine adduct of 13-HPODE. 2) Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44 Immunohistochemical detection of hexanoyl-lysine adduct. 3) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000) Development and characterization of anti HEL monoclonal antibody. 4) Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006) HEL can be detected in saliva samples from patients with Sjogren’s syndrome. 5) Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007) Application to canine serum samples. 6) Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1. Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects. 7) Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008) Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment. 8) Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008) Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma. 9) Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008) Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept. 10) Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008) HEL concentration in tear was increased by brief passive exposure to cigarette smoke. 11) K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato; Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation. Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014) An original paper for HEL ELISA development.

Product name Code Assay range Application Hexanoyl-Lys (HEL) ELISA KHL-700E 2-700 nmol/L Urine, serum, cultured cells and other biological materials Made in Japan.

Related products:&nbsp&nbsp&nbsp Anti Hexanoyl-Lys monoclonal antibody  &nbsp

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual Quality control Technical information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry and ELISA. For research use only.

A new biomarker for lipid peroxidation

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

Cisplatin-treated rat kidney. (Dr. Sugiyama, Tottori Univ.) Detection of HEL at Human atherosclerotic lesions. (Dr. Naito)

Specifications

Clone #: 5F12 Subclass: IgG1 kappa Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH) Specificity: Specific to Hexanoyl-Lys adducts. Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 µg/mL of IgG. Application: Immunohistochemistry (Recommended antibody concentration is 2 µg/mL on paraformaldehyde fixed tissue), and ELISA Storage: Less than -20°C

References

1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999) Identification of HEL, which is lysine adduct of 13-HPODE. 2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000) Development and characterization of anti HEL monoclonal antibody. 3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008) Application to rabbit atherosclerotic lesions. 4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008) Application to cultured cells (HUVECs). 5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008) Application to cultured cell from mouse.

Product name Code Specifications Application NOTICE Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20 µg of IgG Immunohistochemistry Current product Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20 µg of IgG Immunohistochemistry Discontinued Made in Japan. IMPORTANT NOTICE The clone of anti HEL monoclonal antibody has been changed from 5H4 (code. MHL-020P) to 5F12 (code. MHL-021P). If you need old product, please contact our technical support.

Related products:&nbsp&nbsp&nbsp Hexanoyl-Lys ELISA kit  &nbsp

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual (PDF) Immunohistochemical procedure Technical information Comparison of clone 5F12 and 5H4 (PDF)

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry and western blotting. For research use only.

About 4-hydroxy-2-nonenal (4-HNE)

Structure of 4-hydroxy-2-nonenal Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

Specifications

Clone #: HNEJ-2 Immunogen: 4-HNE-modified Keyhole Limpet Hemocyanin Subclass: Mouse IgG1(kappa) Prepared as ascite, and ammnonium sulphate purified. Form: Lyophilized Powder (containing 20 µg or 100 µg of IgG) Specificity: This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde. Applications: Immunohistochemistry and western blotting. Storage: Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature.

References

Immunohistochemistry: T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni: Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997) Western blotting: S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida: The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct. FEBS Lett. 359, p189-191 (1995)

Product name Code Content Anti 4-HNE monoclobal antibody MHN-020P 20 µg of IgG/ vial MHN-100P 100 µg of IgG/ vial Made in Japan

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual (PDF) Immunohistochemical procedure Technical information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry. For research use only.

About malondialdehyde (MDA)

Malondialdehyde (MDA) is one of the major aldehyde derive from lipid peroxidation. MDA is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with MDA and lysine residue leads to the formation of numerous numbers of adducts, such as dihydropyridine-lysine (DHP-lysine) type derivative. This monoclonal antibody is specific for the MDA-modified protein, especially DHP-lysine type derivative.

Specifications

Clone #: 1F83 Antigen: MDA-modified keyhole-lympet hemocyanine. Form: Frozen (100 µg/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A. Application: Immunohistochemistry. Recommended antibody concentration is 0.5-1.0 µg/mL on paraformaldehyde fixed tissue. Specificity: MSpecific for MDA-modified protein (especially DHP-lysine). Subclass: Mouse IgG2a(lambda) Storage: Less than -20°C   Specificity of anti MDA antibody. Immunohistochemical detection of MDA-modified protein in atherosclerotic aorta. Dr. N Shibata, Tokyo Women’s Medical University.

References

1) Immunochemical detection of a lipofuscin-like fluorophore derivered from malondialdehyde and lysine. S Yamada, S Kumazawa, T Ishii, T Nakayama, K Itakura, N Shibata, M Kobayashi, K Sakai, T Osawa and K Uchida. J.Lipid Res. 42, p1187-1196 (2001) 2) Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress. Muratori M, Tamburrino L, Marchiani S, Cambi M, Olivito B, Azzari C, Forti G, Baldi E Mol Med. 21,p109-122(2015). doi: 10.2119/molmed.2014.00158.

Product name Code Content Anti Malondialdehyde (MDA) monoclonal antibody MMD-030n 30 µg IgG Manufactured by NOF corporation, Japan.

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual (PDF) Technical information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry. For research use only.

Acrolein (ACR) is a representative carcinogenic aldehyde found ubiquitously in the environment and formed endogenously through oxidation reactions, such as lipid peroxidation and myeloperoxidasecatalyzed amino acid oxidation. ACR is highly reactive aldehyde and reacts with lysine residue in protein. The reaction with ACR and lysine residue leads to the formation of numerous numbers of adducts, such as formyl-dehydropiperidino-lysine (FDP-lysine) type derivative. This antibody is specific for the ACR-modified protein, especially FDP-lysine type derivative.

Specifications

Clone #: 5F6 Antigen: ACR-modified keyhole-lympet hemocyanine Form: Frozen (100 µg/mL antibody in 10mM PBS containing 0.1% NaN3 and 0.5% BSA). Purified by Protein-A. Application: Immunohistochemistry. Recommended antibody concentration is 0.5-1.0 µg/mL on paraformaldehyde fixed tissue. Specificity: Specific for ACR-modified protein (especially FDP-lysine type derivative) Subclass: Mouse IgG1,kappa Storage: Less than -20°C

References

1) Protein-bound acrolein: Potential markers for oxidativestress. K.Uchida, M.Kanematsu, K.Sakai, T.Matsuda, N.Hattori,Y.Mizuno, D.Suzuki,T.Miyata, N.Noguchi, E.Niki,T.Osawa Proc.Natl.Acad.Sci.USA,95,4882-4887(1998) 2) Protein-bound acrolein: A novel markers of oxidativestress in Alzheimer’s Disease. Noel Y. Calingasan, Koji Uchida, and Gary E.Gibson Journal of Neurochemistry.72(2),751-756(1999)

Product name Code Content Anti Acrolein monoclonal antibody MAR-020n 20 µg of IgG MAR-100n 100 µg of IgG Manufactured by NOF corporation, Japan.

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual (PDF) Technical information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。

Suitable for immunohistocheistry. For research use only.

About 4-hydroxy-2-hexenal (4-HHE)

4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.

Specifications

Clone #: HHE53 Antigen: HHE-modified keyhole-lympet hemocyanine Specificity: Specific for HHE-modified protein (especially HHE-His Michael adduct) Subclass: Mouse IgG1 kappa Prepared as ascite, and protein-A purified. Form: Frozen in 10mM PBS containing 0.1% NaN3 and 0.5% BSA. Application: Immunohistochemistry；It is recommended that the antibody be tried at 0.5-1.0 µg/mL on paraformaldehyde fixed tissue. Storage: Less than -20°C   Specifity of anti 4-HHE antibody.

Product name Code Content Anti 4-HHE monoclonal antibody MHH-030n 30 µg IgG Manufactured by NOF corporation, Japan.

If you are in the U.S., please click here to Genox Corporation (Los Angeles, CA).

Instruction manual (PDF) Technical information

日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。