葡聚糖硫酸钠5,000
Sodium Dextran Sulfate 5,000
规格含量 : Total sulfur : 15.0～20.0%
生产商：FUJIFILM Wako Pure Chemical Corporation
保存条件 : 室温
CAS RN® : 9011-18-1
分子量 : 1,000~9,000
应用级别：分子生物学级别，医药生产用原料级别可供选择
包装规格：25 g、100 g、500 g 可批量供应
◆产品概况
葡聚糖硫酸钠，是一种由葡萄糖聚合，经硫酸化后所得的化合物。本产品的平均分子量约为5000（分子量范围：1000-9000），是聚阴离子物质。在细胞培养中，本品可通过改变细胞表面的电荷，来促使单个细胞悬浮，防止细胞结团，是一种被广泛用于重组蛋白生产的抗结团剂。
本品有分子生物学级别与用于医药生产的CertiPro-L级别两种可供选择。分子生物学级别经电泳或荧光法对DNase和RNase的活性进行检测。CertiPro-L级别是GMP标准下生产的符合日本药典、医药品添加剂规格（药添规）标准的生产专用原料。联系富士胶片和光客服可获取相应产品规格书。
◆物性信息
外观：白色~微淡黄色，结晶粉末~粉末
溶解性：易溶于水，几乎不溶于乙醇和丙酮
pH信息：pH（50 g/L，25℃）：4.5～8
包装：氩气封装
◆用途
可防止细胞培养中的细胞结团，提升CHO细胞等生产用细胞系的重组蛋白产量。
其他用途
1. 诱导小鼠、大鼠等动物的结肠炎模型[1-12]；
2. 诱导小鼠的结肠癌模型[13]；
3. β-脂蛋白的定量分析中选择性分离脂蛋白[14]；
4. 抑制人免疫缺陷病毒（HIV）的体外复制[15]；
5. 细胞培养中防止细胞结团；
6. 促进核酸杂交。
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参考文献
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1. |
Hori, Y. , Hoshino, J. , Yamazaki, C. , Sekiguchi, T. , Miyauchi, S. , & Mizuno, S. , et al. (1997). Effect of lecithinized-superoxide dismutase on the rat colitis model induced by dextran sulfate sodium. Jpn J Pharmacol, 74(1), 99-103. DSS 5000溶解于蒸馏水中并制备成3%溶液（25mg/5ml/Kg）【IF=1.305，诱导大鼠结肠炎模型】 |
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2. |
Kawabata, K., Kanmura, S., Morinaga, Y., Tanaka, A., Makino, T., Fujita, T. … Ido, A. (2019). A high‑fructose diet induces epithelial barrier dysfunction and exacerbates the severity of dextran sulfate sodium‑induced colitis. International Journal of Molecular Medicine, 43, 1487-1496. 全文 DSS 5000溶解于蒸馏水中并制备成1%溶液【IF=3.098，诱导小鼠结肠炎模型】 |
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3. |
Numata, Y. , Tazuma, S. , Nishioka, T. , Ueno, Y. , & Chayama, K. . (2010). Immune response in mouse experimental cholangitis associated with colitis induced by dextran sulfate sodium. Journal of Gastroenterology & Hepatology, 19(8), 910-915. DSS 5000溶解于蒸馏水中并制备成2.5%溶液【IF=1.83，诱导小鼠结肠炎模型】 |
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4. |
Ogawa, A., Andoh, Araki, Y., & Bamba, et al. (2004). Neutralization of interleukin-17 aggravates dextran sulfate sodium-induced colitis in mice. CLINICAL IMMUNOLOGY, 110(1), 55-62. 向小鼠喂食含有2.0%(wt/wt) DSS 5000的饲料【IF=3.368，诱导小鼠结肠炎模型】 |
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5. |
Nishimura, T. , Andoh, A. , Hashimoto, T. , Kobori, A. , Tsujikawa, T. , & Fujiyama, Y. . (2010). Cellobiose prevents the development of dextran sulfate sodium (dss)-induced experimental colitis. Journal of Clinical Biochemistry and Nutrition, 46(2). 向小鼠喂食含有3.5%(wt/wt) DSS 5000的饲料【IF=2.405，诱导小鼠结肠炎模型】 |
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6. |
Deguchi, Y., Andoh, A., Inatomi, O., Yagi, Y., Bamba, S., Araki, Y., Hata, K., Tsujikawa, T., & Fujiyama, Y. (2007). Curcumin prevents the development of dextran sulfate Sodium (DSS)-induced experimental colitis. Digestive diseases and sciences, 52(11), 2993–2998. 全文 向小鼠喂食含有3.5%(wt/wt) DSS 5000的饲料【IF=2.751，诱导小鼠结肠炎模型】 |
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7. |
Araki, Y., Mukaisyo, K., Sugihara, H., Fujiyama, Y., & Hattori, T. (2010). Increased apoptosis and decreased proliferation of colonic epithelium in dextran sulfate sodium-induced colitis in mice . Oncology Reports, 24, 869-874. 全文 向小鼠喂食含有5%(wt/wt) DSS 5000的饲料【IF=3.417，诱导小鼠结肠炎模型】 |
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8. |
Katada, K., Yoshida,N., Suzuki,T., et al. (2008). Dextran sulfate sodium-induced acute colonic inflammation in angiotensin ii type 1a receptor deficient mice. Inflammation Research, 57(2), 84-91. 用饮用水向小鼠喂食3.0 % (w/vol) DSS 5000，持续7天【IF=3.174，诱导小鼠结肠炎模型】 |
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9. |
Iwanaga, T. , Hoshi, O. , Han, H. , & Fujita, T. . (1994). Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: some possible mechanisms of cecal ulceration. Journal of Gastroenterology, 29(4), 430. 向豚鼠喂食3.0 % DSS 5000水溶液【IF=6.132，诱导豚鼠结肠炎模型】 |
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10. |
Shintani, N. , Nakajima, T. , Okamoto, T. , Kondo, T. , Nakamura, N. , & Mayumi, T. . (1998). Involvement of cd4+ t cells in the development of dextran sulfate sodium-induced experimental colitis and suppressive effect of igg on their action. General Pharmacology: The Vascular System, 31(3), 477-481. 向小鼠喂食含有5.0 % (w/v) DSS 5000的饮用水，持续7天【IF=1.134，诱导小鼠结肠炎模型】 |
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11. |
Kanauchi, O. , Nakamura, T. , Agata, K. , Mitsuyama, K. , & Iwanaga, T. . (1998). Effects of germinated barley foodstuff on dextran sulfate sodium-induced colitis in rats. Journal of Gastroenterology, 33(2), 179-188. 向大鼠喂食含有3.0%(wt/wt) DSS 5000的饲料【IF=6.132，诱导大鼠结肠炎模型】 |
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12. |
Kao, N. J. , Hu, J. Y. , Wu, C. S. , & Kong, Z. L. . (2016). Curcumin represses the activity of inhibitor-κb kinase in dextran sulfate sodium-induced colitis by s-nitrosylation. International immunopharmacology, 38, 1-7. 向小鼠喂食含有5.0 % (w/v) DSS 5000的饮用水【IF=3.943，诱导小鼠结肠炎模型】 |
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13. |
Gu, S. , Papadopoulou, N. , Gehring, E. M. , Nasir, O. , Dimas, K. , & Bhavsar, S. K. , et al. (2009). Functional membrane androgen receptors in colon tumors trigger pro-apoptotic responses in vitro and reduce drastically tumor incidence in vivo. Molecular Cancer, 8(1), 114. 向小鼠喂食30g/L（即3.0%）的 DSS 5000水溶液，持续7天【IF=15.302，诱导小鼠结肠癌模型】 |
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14. |
Sakagami, T. , & Zilversmit, D. B. . (1961). Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis. Journal of Lipid Research, 2(3), 271-277. 向每毫升血清中加入0.04 ml的5％葡聚糖硫酸盐和0.1 mL的11％氯化钙。在4℃下静置2小时后，将混合物以4,000 X g的速度离心10分钟，去除上清液【IF=4.483，选择性分离脂蛋白】 |
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15. |
Ito, M. , Baba, M. , Sato, A. , Pauwels, R. , Clercq, E. D. , & Shigeta, S. . (1987). Inhibitory effect of dextran sulfate and heparin on the replication of human immunodeficiency virus (hiv) in vitro.Antiviral Research , 7(6), 361-367. 使用PBS溶解（保存于4℃待用）【IF=4.101，抑制人免疫缺陷病毒（HIV）的体外复制】 |
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16. |
Yoshio, A., kira, A., Yoshihide, F., Kazunori, H.,Jin, M., Takafumi, O., Fumiyasu, N., Tadao, B., et al. (2001). Application of 2-aminopyridine fluorescence labeling in the analysis of in vivo and in vitro metabolism of dextran sulfate sodium by size-exclusion high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 753( 2), 209-215. 0.2mol的 DSS 5000溶解于25mL的氨基吡啶溶液中【利用HPLC分析在体内外的代谢】 |