Wako 019-19741 Anti Iba1, Rabbit

发表于由

Wako 和光纯药 Anti Iba1, Rabbit (for Immunocytochemistry)

Iba1抗体，兔源（免疫細胞化学用）

品牌 产品编号 产品名称 包装
WAKO 019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50ug

钙离子作为信号转导通道中的第一信使，在所有细胞包括中枢神经细胞的生命活动中起重要作用。钙离子与不同的钙结合蛋白结合后进行级联反应。Iba1是能与巨噬细胞和小胶质细胞发生特异性结合的钙结合蛋白，当这些细胞激活后，Iba1的表达量上调，因此Iba1通常作为鉴定小胶质细胞的标记物使用。近来研究发现，小胶质细胞除了提供营养、保护神经的作用外，还被证明可产生NO、TNF-a、IL-1β等物质，因此将小胶质细胞定义为中枢神经系统的巨噬细胞样吞噬细胞，具有重要的免疫细胞作用。当病原菌、病毒入侵中枢神经系统时会起免疫监御的作用。近来研究发现，该细胞可能与神经退行性疾病的发生和发展有关。本产品是可与小胶质细胞发生特异性反应的兔多抗，配合对星形胶质细胞有特异性的GFAP单抗，可进行双染色。

抗原：合成肽（Iba1 的C末端序列）

外观：TBS溶液（1mg/ml）

提纯：抗原亲和提纯

特异性：对小胶质细胞、巨噬细胞特异反应。不会与神经元、星形胶质细胞发生交叉反应。能与人、小鼠、大鼠Iba1反应。

用法：IBA1抗体，兔源（免疫細胞化学用）适合免疫細胞化学。只需1~2μg/ml即可进行实验。

  • Anti Iba1, Rabbit

    019-19741(For Immunocytochemistry) & 016-20001 (for Western Blotting)

    Iba1: ionized calcium binding adapter molecule 1 小胶质细胞/巨噬细胞特异性蛋白抗体

    钙离子是已知的所有细胞，包括中枢神经系统（CNS）细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动，其中许多钙结合蛋白被划分成一个大的蛋白家族，EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白，在巨噬细胞和小胶质细胞中表达，并在这些细胞的活化过程中表达升高。
    本抗体以合成的的Iba1羧基末端序列为抗原，该序列是人，大鼠和小鼠Iba1蛋白质保守序列。本抗体（免疫组化用）特异性与小胶质细胞/巨噬细胞反应，免疫双重染色与单克隆抗体结合，可与GFAP单克隆抗体（(胶质纤维酸性蛋白，Glial Fibrillary Acidic Protein）联合使用对脑组织和细胞培养物进行双重染色。

    特点

    S特异性检测小胶质细胞与/巨噬细胞，不与神经细胞与星形胶质细胞发生交叉反应。可与人，大鼠，小鼠的Iba1反应

    货号 品名 包装 用途 工作浓度
    019-19741 Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry 50ug(100ul) IH 1-2ug/ml
    016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting 50ug(100ul) WB 0.5-1ug/ml

    应用

    石蜡切片应用方法：
    1. 固定
    2. 用0.01M PBS缓冲液清洗二次
    3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
    4. 用0.01M PBS缓冲液清洗三次
    5. 使用封闭液（含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液）室温孵育2小时
    6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
    7. 用0.01M PBS缓冲液清洗三次
    8. 使用含有生物素标记的抗兔IgG（1：200）的封闭液室温孵育1小时
    9. 用0.01M PBS缓冲液清洗三次
    10. 使用Elite ABC试剂（含有试剂A1：50，试剂B1：50的0.01M PBS溶液）
    11. 用0.01M PBS缓冲液清洗三次

    12. 过氧化物酶溶液（含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液）

    英文参考：

    Summary

    This product is for research use only. Do not administer it to human.

    Antibodies against Macrophage/Microglia-specific Protein Iba1

    Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.

    Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1.

    Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.

    Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.

    Working Concentration: Immunocytochemistry 1-2 micro-g/mL

    保存方法：Storage:Store at -20 degrees C. After opening, aliquot contents and freeze at -20 degrees C.

    在-20℃下储存。打开后，等分内容物并在-20℃下冷冻。

    Package Size: 50 ug (100 uL) (ug: micro-g; uL: micro-L)

    References:

    Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).

    Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).

    Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).

    Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).

    Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).

    Wako catalog #016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting (50 micrograms (100 microlitters))Working Concentration: Westernblot 0.5 – 1 micrograms/mL

    [Recommended preparations]

    Frozen mouse brain tissue or cultured cells should be perfusion-fixed with 4% paraformaldehyde-PBS. Then the tissue section is prepared. After those preparation, the tissue section and cultured cells are immunostained with anti Iba1 antibody.

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