Wako 071-02293 γ-Globulin, from Human Serum9007-83-4
标签归档:日本和光纯药
Wako 065-06651 2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺 3688-53-7
Wako 065-06651 2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺 3688-53-7
英文名称:2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide
中文名称:2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺
品牌:Wako
CAS No.:3688-53-7
储存条件:室温
纯度:98.0+% (HPLC)
065-06651 |
2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide | Wako Special Grade | 50 mg | 3688-53-7 |
Wako 051-08411 Evodiamine Standard 吴茱萸碱标准品 替代品:059-09311
Wako 051-08411 Evodiamine Standard 吴茱萸碱标准品 替代品:059-09311
Wako代理 017-12101 1-Anthroyl Cyanide 1-氰酸蒽 85985-43-9
Wako代理 017-12101 1-Anthroyl Cyanide 1-氰酸蒽 85985-43-9
017-12101 |
1-Anthroyl Cyanide
1-氰酸蒽 |
for Hydroxy Groups Fluorescent Labeling | 100 mg | 85985-43-9 |
Wako和光纯药297-51501 SLP Reagent Set 3mL 替代品:296-81001
Wako和光纯药297-51501 SLP Reagent Set 3mL 替代品:296-81001
——肽聚糖和(1→3)-β-D-葡聚糖定量检测
家蚕的淋巴有一种自我保护机制,被称为“酚氧化酶原串联反应”。当受外界入侵(如细菌和真菌)时,
这个串联反应参与黑色素的形成并使机体免受攻击。这个反应由细菌中的肽聚糖或是真菌、酵母中的(1→
3)-β-D-葡聚糖引发,该反应中的酚氧化酶原被激活。也有人认为丝氨酸蛋白酶也参与了该反应,但是没
有从这方面阐述的资料。
已消毒的SLP包括了所有参与该反应的因子,再生后的溶液中含有L-3,4-苯丙氨酸(L-3,4-
dihydroxyphenylalanine, DOPA),该物质可以作为肽聚糖或β-葡聚糖所激发的酚氧化酶的底物,DOPA被
酚氧化酶氧化,形成黑色素。肽聚糖是绝大多数细菌细胞壁的组成成分,而β-葡聚糖广泛的分布于真菌
的细胞壁中。因此,SLP通过测定黑色素的形成情况,进而定性或定量检测细菌和真菌的污染。
特点
1. 用于肽聚糖和(1→3)-β-D-葡聚糖的定量检测,检测敏感。
2. 使用酶标仪或内毒素检测仪,能对肽葡聚糖和β-葡聚糖精确定量。
3. 不需任何特殊器械即可通过肉眼检测出细菌和真菌的污染。
4. 冻干SLP的在2-10℃至少可保存18个月。
原理见:http://www.cxbio.com/news/news_details.asp?id=9588
当使用酶标仪或内毒素检测仪时,通过测定反应的始动时间和由颜色变化而导致的吸光度的变化,即可对
肽聚糖或β-葡聚糖进行定量。
所含试剂(所有试剂均存放于2-10℃):
1. 冻干SLP for 3ml
使用酶标仪或者目测约50Test,使用内毒素检测仪约30Test
2. 冻干底物 for 3ml
含有作为酚氧化酶底物的DOPA
3. 稀释液 4ml
含有good’s buffer
试剂准备:
(A) 底物中加入3ml稀释液,轻轻摇晃至底物完全溶解,注意避免碰到橡胶帽。
(B) 所有底物溶液加入冻干的SLP中,轻轻摇晃至SLP完全溶解,同样,注意避免碰到橡胶帽。此溶液即为
SLP检测液。
操作步骤
1. 使用酶标板(微孔板)目测
(A) 分别稀释肽聚糖和β-葡聚糖参照物,做两个不同浓度的参照系列,每孔加入0.05ml的参照物稀释液
或者待测样品
(B) 每孔加入0.05mlSLP检测液
(C) 温和摇晃后,酶标板于30℃孵育一小时
(D) 肉眼判断:反应液中有黑色素生成的为阳性,其它的为阴性。
(E) 参考物的最低浓度(即能生成黑色素的最小浓度)不应在SLP检测液的最低检测浓度以下。
(F) 样品中SLP反应物浓度是否在SLP检测液浓度的最低检测范围内,决定了样品是阳性或阴性。
2. 使用酶标仪检测
(A) 样品和参考物的稀释系列各0.05ml分别加入酶标板孔中
(B) 迅速在每孔中加入0.05mL SLP检测液。好用消毒过的加液器。
(C) 在以下条件下使用酶标仪上测定光吸收值:
检测波长: 650nm
检测模式: 动态检测
反应时间: 90分钟
检测温度: 30℃
自动混匀: 一次
(D) 绘制双对数回归(regression of Log-Log)标准曲线,横轴为SLP参考物的浓度,坚轴为反应时间
(E) 通过标准曲线上的反应始动时间,计算SLP反应物质的浓度。
3. 使用内毒素检测仪做SLP检测,其操作步骤可以按照仪器生产商提供的操作手册中所述的细菌内毒素检
测方法操作。内毒素检测仪可能会发出反应混合液缺乏透射光的信号。忽视这个的警告,测定结果不会受
此影响。
297-51501 SLP Reagent Set (定量的需要另配以下标准品使用)
162-18101 Peptidoglycan, from Micrococcus luteus肽聚糖 2ml
030-09903 Curdlan (1→3)-β-D-葡聚糖 1g
1. 器械应250℃干热灭菌至少2小时,并避免被SLP反应物污染
2. SLP冻干粉溶解后,其溶液应存于0-4℃,并尽早使用
3. 注意在器械储存期间避免其被SLP反应物质污染
4. SLP检测液的敏感性很大程度上受离子强度的影响
5. 该试剂盒仅用于研究,不可用于诊断;仅用于体外,不可用于人或动物体内。
(本资料只供参考,以原包装说明书为准)
常见使用说明:
1. 因为SLP是被PG(肽聚糖)或BG[(1→3)-β-D-葡聚糖]所引发,在操作和使用本试剂时,单一灭
菌器皿或者使用无热源器皿都不够的,必须还要同时无PG和BG;
2. 如果是用玻璃器皿,清洗干净后,需要在250度下干热灭菌2小时;
3. 如果是一次性无菌器皿,在使用之前必须进行测试能否适用于此项检测(即注1);
4. 如果使用过后还有剩余,我们认为其很可能被样品污染,不建议继续使用。以前我们也遇到类似
的问题,但所有的都是因为污染造成。如果用户一定要保留一些SLP溶液,建议在未使用前进行无菌分装
并0-4℃保存;好是将SLP冻干粉在无菌无污染条件下,按照使用计划量预先分装后0-4℃保存。
297-51501 SLP Reagent Set 3mL用
293-58301 SLP-HS Single Reagent Set 20回用
198-01055 Sodium Acetate Trihydrate 500g
194-13321 Sodium Alginate 80-120 100g
198-01675 Sodium Chloride 500g
199-08361 Sodium Dextran Sulfate 5,000
194-13402 Sodium Dextran Sulfate 5000 25g
197-09705 Sodium Dihydrogenphosphate 500g
192-02815 Sodium Dihydrogenphosphate Dihydrate 500g
190-02811 Sodium Dihydrogenphosphate Dihydrate 100g
192-07935 Sodium Hydroxide 500g
197-03345 Sodium Sulfate 500g
197-07125 Sodium Sulfate 500g
191-03985 Soluble Starch 和光一級 500g
199-16291 Stevioside Standard 100mg
196-00015 Sucrose 500g
191-04502 Sulfanilamide 25g
204-08343 Testosterone 5g
203-00432 Tetraethylammonium Bromide 25g
205-00431 Tetraethylammonium Bromide 100g
208-16771 TFNG Standard 20mg
124-02275 Trilithium Citrate Tetrahydrate 500g
206-15591 Trinexapac Standard 200mg
190-05535 Trisodium Citrate Dihydrate 500g
208-09223 Trypsin Inhibitor, from Soybean 1g
028-03386 t-Butyl Alcohol 500ml
223-02112 VA-044 25G
226-01306 Vancomycin Hydrochloride 5g
302-07111 Vitamin E Reference Standard 1kit
234-01565 WakogelR 50C18 500g
396-01731 XLD 日局試験用 300g
035-17981 α-Carotene Standard 10mg
354-03232 γ-Phenylpropyl Acetate 25g
242-00641 Xylobiose 100mg
334-30183 Z-Glu-Tyr 1g
263-00271 Zinc Chloride 10g
033-08332 α-Cyclodextrin 25g
034-13632 Chitin 25g
030-09903 Curdlan 1g
342-01875 EDTA-3NA 500g
030-05525 Citric Acid 500g
192-09071 Silicon Dioxide, 99.9% 100g
192-06031 Silicon Standard Solution 100ml
194-00832 Silver Nitrate 25g
196-12781 Simeconazole Standar 200mg
Wako 013-08391 H型氨基酸混合标准液 替代品:013-28154
Wako 013-08391 H型氨基酸混合标准液 替代品:013-28154
Wako新型PS亲和法Exosome外泌体纯化试剂盒
Wako新型PS亲和法Exosome外泌体纯化试剂盒
外泌体和其他细胞外囊泡(EVs)属于小细胞膜囊泡,包含蛋白,mRNA,microRNA,DNA和脂类,是由不同细胞分泌,并且在体液包括血液、唾液、 尿液、脑脊髓液(CSF)和乳液中稳定存在。这些EVs被当做细胞和细胞沟通的信使,不同疾病的生物标志物。提取外泌体的常规方法有:超离,使用表面抗原 的抗体进行亲和层析,和用多聚物试剂进行沉淀。然而,这些方法在回收效率,纯度和可操作性上不是非常令人满意。
MagCapture™Exosome Isolation Kit PS采用磁珠和磷脂酰丝氨酸(PS)结合蛋白(PS亲和法)的方法进行纯化。该方法可以很方便获得高纯度的外泌体和其他来自细胞培养基和体液的EVs(通 过正常的微滤方法获得高产量)。如果想要获得更高纯度的外泌体,请使用10,000×g获得上清。该试剂盒利用中性pH的金属螯合试剂,将捕获的EVs从 磁珠上洗脱下来,可以获得完整的外泌体和其他EVs。纯化的完整外泌体和其他EVs可用于不同实验,包括电镜分析,纳米粒子追踪分析,EVs的操作和分析。
细胞膜表面磷脂酰丝氨酸的亲和方法
使用磷脂酰丝氨酸(PS)结合蛋白,细胞外囊泡在金属离子依赖方式捕获,接着用金属粒子螯合试剂进行洗脱。
◆优点・特色
使用新型亲和纯化方法
● 通过PS亲和分子回收
● 低背景值 ● 在中性条件下通过螯合试剂进行温和洗脱 ※ 可获得高纯度,完整的外泌体 |
|
无需进行超离
● 使用磁珠改进了操作
● 流程优化
※ 高重复性
与其他纯化方法相比
方法 | 纯度 | 外泌体回收率 | 获得囊泡的完整性 |
MagCapture™
(PtdSer亲和方法) |
◎ | ■■■ | ○ |
超离 | △ | ■■ | ○ |
多聚物沉淀 | × | ■■■■ | ○ |
外泌体表面抗原亲和方法(使用抗体) | ○ | ■ | × |
样品类型:细胞培养上清,血清,血浆,尿液等。
◆产品列表
产品名称 | 包装规格 | 和光编号 | 保存条件 |
MagCapture™ Exosome Isolation Kit PS |
10次 | 293-77601 | 保存在2-10°C |
试剂盒成分
(1) 链霉亲和素磁珠 |
<10 次> 600 μL x 1 管 100 μL x 1 管 35 mL x 1 瓶 500 μL x 1 管 75 mL x 2 瓶 5 mL x 1 瓶 22 管 |
*每个test里的磁珠可以循环使用共5次,即试剂盒的实际使用量为:10tests*5次/Kit
产品编号 | 产品名称 | 产品规格 |
293-77601 | MagCapture™ Exosome Isolation Kit PS MagCapture™ 外泌体提取试剂盒PS |
10次用 |
299-77603 | MagCapture™ Exosome Isolation Kit PS MagCapture外泌体提取试剂盒PS |
2次用 |
297-79201 | PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) PS Capture™外泌体ELISA试剂盒(抗小鼠IgG POD) |
|
品牌 | 产品编号 | 产品名称 | 等级 | 规格 |
Wako | 299-77603 | MagCapture™ Exosome Isolation Kit PS MagCapture™外泌体提取试剂盒PS | for Genetic Research | 2 tests |
Wako | 293-77601 | MagCapture™ Exosome Isolation Kit PS MagCapture™外泌体提取试剂盒PS | – | 10 tests |
Wako | 290-84103 | MagCapture™ Exosome Isolation Kit PS Ver.2 MagCapture™外泌体提取试剂盒PS Ver.2 | for Genetic Research | 10 tests |
Wako | 294-84101 | MagCapture™ Exosome Isolation Kit PS Ver.2 MagCapture™外泌体提取试剂盒PS Ver.2 | for Genetic Research | 2 tests |
Wako | 297-79201 | PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD) PS Capture™外泌体ELISA试剂盒(抗小鼠IgG POD) | – | 96 tests |
Wako | 297-79701 | PS Capture™ Exosome Flow Cytometry Kit PS Capture™外泌体流式试剂盒 | for Genetic Research | 300 tests |
Wako | 298-80601 | PS Capture™ Exosome ELISA Kit (Streptavidin HRP) PS Capture™外泌体ELISA Kit (链霉亲和素HRP) | for Genetic Research | 96 tests |
Wako | 290-80301 | PS Capture™ Exosome Isolation Resin Kit PS Capture™外泌体提取树脂试剂盒 | for Genetic Research | 1 kit(0.5 mL浆状) |
Wako | 052-09301 | Exosomes, from COLO201 cells, purified COLO201来源外泌体(已纯化) | for Genetic Research | 50 μL |
Wako | 292-83801 | CD81-Capture Human Exosome ELISA Kit (Streptavidin HRP) CD81-Capture 人外泌体ELISA试剂盒(链霉亲和素HRP) | for Genetic Research | 96 tests |
组织透明化原理
细胞膜表面磷脂酰丝氨酸的亲和方法
外泌体提取试剂盒
使用磷脂酰丝氨酸(PS)结合蛋白,细胞外囊泡在金属离子依赖方式捕获,接着用金属粒子螯合试剂进行洗脱
外泌体提取试剂盒的优点/特色
使用新型亲和纯化方法!
可获高纯度,完整的外泌体!
无需进行超离 !
组织透明化检测流程
产品编号 | 产品名称 | 产品规格 | 零售价 | |
293-77601 | MagCaptureTM Exosome Isolation Kit PS Exosome外泌体纯化试剂盒 |
10次 | 8980 |
*每套试剂盒能完成5次回收实验,即实际使用量为10×5次/Kit
Wako 299-79901 组织透明化试剂盒 SCALEVIEW-S Trial Kit
Wako 299-79901 组织透明化试剂盒 SCALEVIEW-S Trial Kit
The original recipe reported by the Miyawaki team in 2011 termed Scale was an aqueous solution based on urea that is limited because the transparency process itself can damage the structures under study.
The research team spent 5 years improving the effectiveness of the original recipe to overcome this critical challenge, and the result is ScaleS, (we called SCALEVIEW-S) a new technique with many practical applications. SCALEVIEW-S creates transparent brains with minimal tissue damage, that can handle both florescent and immunohistochemical labeling techniques, and is even effective in older animals.
The new technique creates transparent brain samples that can be stored in SCALEVIEW-S solution for more than a year without damage. Internal structures maintain their original shape and brains are firm enough to permit the micron-thick slicing necessary for more detailed analyses.
Miyawaki 团队在 2011 年报告的原始配方称为 Scale 是一种基于尿素的水溶液,它是有限的,因为透明过程本身会损坏正在研究的结构。
研究团队花了 5 年时间改进原始配方的有效性以克服这一关键挑战,结果是 ScaleS,(我们称为 SCALEVIEW-S)一种具有许多实际应用的新技术。 SCALEVIEW-S 创造了组织损伤最小的透明大脑,可以处理荧光和免疫组织化学标记技术,甚至对老年动物也有效。
新技术创造了透明的大脑样本,可以在 SCALEVIEW-S 解决方案中存储一年多而不会损坏。 内部结构保持其原始形状,大脑足够坚固,可以进行更详细分析所需的微米厚切片。
Overview / Applications
Outline | This product is for research use only. Do not administer it to human. SCALEVIEW-S series are reagent used for ScaleS. ScaleS is tissue clearing method developed by Dr. Atsushi Miyawaki etc. This method uses solutions with mainly urea. The tissue is made transparent and adjusted RI for microscopic observation by soaking in 6 kinds of solutions (SCALEVIEW-S0, SCALEVIEW-S1, SCALEVIEW-S2, SCALEVIEW-S3, SCALEVIEW-S4, SCALEVIEW-SMt). 2mm section can be made transparent in 1 day using the reagents. The method can make a transparent tissue keeping the structure of proteins. Therefore it is possible to observe the tissue expressing fluorescent proteins, and to label by antibody and/or fluorescent dye after tissue clearing. It’s also applicable to not only mouse and rat brain but also human after dye. The transparent tissue can be observed for electric microscope or confocal microscope. ScaleS is higher transparency than ScaleA2. See the following website for more details. |
---|
概述 / 应用
本产品仅供研究使用。不要将其施用于人类。
SCALEVIEW-S 系列是用于 ScaleS 的试剂。 ScaleS 是宫胁敦博士等开发的组织清除方法。该方法使用以尿素为主的溶液。
通过浸泡在 6 种溶液(SCALEVIEW-S0、SCALEVIEW-S1、SCALEVIEW-S2、SCALEVIEW-S3、SCALEVIEW-S4、SCALEVIEW-SMt)中使组织透明并调整 RI 用于显微观察。 2mm 切片可以在 1 天内使用试剂变透明。
该方法可以制成保持蛋白质结构的透明组织。因此可以观察表达荧光蛋白的组织,并在组织清除后用抗体和/或荧光染料进行标记。它不仅适用于小鼠和大鼠的大脑,也适用于染色后的人类。透明组织可用电镜或共聚焦显微镜观察。
ScaleS 的透明度高于 ScaleA2。
有关详细信息,请参阅以下网站。
* 清洁液 deScale Solution (041-34425) 单独出售。
Product List
Product Name | |
---|---|
SCALEVIEW-A2 | Tissue Optical Clearing Reagent |
SCALEVIEW-S0 | Tissue Optical Clearing Reagent |
SCALEVIEW-S1 | Tissue Optical Clearing Reagent |
SCALEVIEW-S2 | Tissue Optical Clearing Reagent |
SCALEVIEW-S3 | Tissue Optical Clearing Reagent |
SCALEVIEW-S4 | Tissue Optical Clearing Reagent |
SCALEVIEW-SMt | Tissue Optical Clearing Reagent |
deScale Solution | Tissue Optical Clearing Reagent |
References 参考文献
- Hama,H.et al. : Nature Neuroscience 14, 1481(2011).
- Hama,H.et al. : Nature Neuroscience 18, 1518(2015).
- Hama H, et al. : Protocol Exchange (2016), doi:10.1038/protex.2016.019
- Molly E, Boutin, et al :Scientific Reports, 8, 11135 (2018).
Wako 290-80801 CUBIC Trial Kit CUBIC试剂盒
Wako 290-80801 CUBIC Trial Kit CUBIC试剂盒
CUBIC Trial Kit
Tissue Optical Clearing Reagent Manufacturer : FUJIFILM Wako Pure Chemical Corporation Storage Condition : Keep at RT.
This product is for research use only. Do not administer it to human.
CUBIC Trial Kit is the reagent kit used for CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) that is clearing method. This method was developed by Professor Hiroki Ueda and Associate Professor Etsuo Susaki etc.
A tissue can be made transparent only soaking into ScaleCUBIC-1 and ScaleCUBIC-2 included in the kit. Also, it’s possible to adjust RI and make microscopic observation by using a reagent that is mixed Mounting Solution-1 and Mounting Solution-2 included the kit. Mouse whole brain can be made transparent in 2 weeks.
CUBIC enables transparency and observation mouse whole tissue including brain, primates such as marmosets whole brain and fish or crustacean whole tissue. This method is expected to be applied pathological approach or to observation of single cell level.
本产品仅供研究使用。 不要将其施用于人类。
CUBIC Trial Kit 是用于 CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) 的试剂盒,是一种清除方法。 该方法由上田博树教授和须崎悦男副教授等开发。
只有浸泡在套件中包含的 ScaleCUBIC-1 和 ScaleCUBIC-2 中才能使组织透明。 此外,通过使用试剂盒中包含的 Mounting Solution-1 和 Mounting Solution-2 混合的试剂,可以调整 RI 并进行显微观察。 小鼠全脑可在 2 周内变得透明。
CUBIC 可实现透明和观察小鼠全组织,包括大脑、灵长类动物(如狨猴全脑和鱼类或甲壳类动物全组织)。 该方法有望应用于病理方法或单细胞水平的观察。
Whole-brain / body imaging with single-cell resolution
CUBIC
Dr. Hiroki R. Ueda et al. developed a simple, efficient, and scalable brain / body clearing method and imaging / computational analysis pipeline, CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis). CUBIC offers a fluorescent-preserving, high-performance and device-free tissue clearing method based on hydrophilic Solutions (ScaleCUBIC Solutions).
It enables reproducible whole-organ and whole-body clearing and rapid 3D imaging with LSFM. CUBIC also provides processing and analysis of 3D images for extracting biological information.
Therefore, CUBIC presents a platform of whole-organ/body imaging with single-cell resolution and image informatics, which enables a wide range of users to perform experiments targeting cellular and organ layers with multiple samples..
Data provided by Dr. Etsuo A Susaki, and Dr. Hiroki R. Ueda, RIKEN
Features
- Easy-to-use
- No special equipment required
- Follow up 2D H&E
- High transparency
- Applicable for many organs
- Compatible with IF, FP and other fluorescent labels
Protocol
Whole organ samples
2 mm section samples
Fluorescent protein expression tissue
Whole organ samples
**Typically, 3:7 mixture of Mounting Solution 1 and 2 (RI ~1.49) can be used.
Brain | Heart | Lung | Kindney | Liver | |
PBS | |||||
CUBIC |
Figure1.Transmittance images of mouse whole organs before and after clearing with Scale CUBIC Solutions.
2 mm section samples
**Typically, 3:7 or 2:8 mixture of Mounting Solution 1 and 2 (RI ~1.49) can be used
Brain | Heart | Liver | |
PBS | |||
CUBIC |
Figure2.Transmittance images of mouse 2mm slices before and after clearing with Scale CUBIC Solutions.
Fluorescent protein expression tissue
Figure3. LSFM imaging of CAG-EGFP Tg Mouse (8w Male) whole brain, whole heart and dissected liver.
Application
CUBIC Application with pathological methods
CUBIC Application with fish clearing
CUBIC Application with crustacean clearing
CUBIC Application with beetle clearing
Visualization of whole-brain vascular networks
CUBIC Application with pathological methods
Data by S. Nojima.et al. : Scientific Reports ,7,9269 (2017) / Adapted.
Before CUBIC
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After CUBIC ——————————————————————————————- |
||||||
0 day | 2 days | 3 days | 4 days | 6 days | 7 days | ||
Human lung |
CUBIC | ||||||
HE stain | |||||||
Human Lymph node |
CUBIC | ||||||
HE stain |
Figure 4.Tissue clearing of various human organs with CUBIC
Tissue clearing and 3D imaging of deparaffinizedsamples with CUBIC
Figure 5.Tissue clearing and 3D imaging of pathological specimens with CUBIC.
CUBIC Application with fish clearing
Data were provided by Kunihiko Futami, Tokyo University of Marine Science and Technology.
Figure6. Fish clearing with CUBIC
See Reference 5) for the protocol
Figure7. Stained image of Fish organ treated by CUBIC
Organs were sliced at 5 μm and stained with Mayer’s hematoxylin after CUBIC treatment.
Figure8. Immunostaining image of zebrafish treated with CUBIC
CUBIC Application with crustacean clearing
Data were provided by Aru Konno and Shigetoshi Okazaki, Hamamatsu University School of Medicine in Japan.
Figure9. Crustacean clearing with CUBIC
See Reference 6) for the protocol
Figure10. Crustacean clearing with CUBIC (PI staining)
See Reference 6) for the protocol
CUBIC Application with beetle clearing
Data were provided by Shinya Kuroda, Tokyo University
Beetle clearing requires fixing, bleaching, and clearing steps.
See the website for Detailed protocols.
Figure11. Beetle clearing with CUBIC
LEFT: drone beetle MIDDLE: rhinoceros beetle RIGHT: stag beetle
This protocol altered from reference 7).
Visualization of whole-brain vascular networks
Data by Miyawaki, T. et al. : Nat. Commun., 11:(1) (2020)
Figure12. Tissue clearing with sodium deoxycholate (SDC)
TOP: SDS-based delipidation. The tissue was swelling.
BOTTOM: SDC-based delipidation. Tissue swelling was suppressed.
This protocol altered from reference 8).
Figure13. Mouse brain cortex clearing after vascular casting
LEFT: SDS-based delipidation. Some penetrating vessels were severed (arrowheads).
BOTTOM: SDC-based delipidation. Breakages of vessels were rarely.
This protocol altered from reference 8).
References 参考文献
- E. A. Susaki. et al. : Cell, 157(3), 726 (2014).
- K. Tainaka. et al. : Cell, 159(4), 911 (2014).
- E. A. Susaki. et al. : Nature Protocols, 10, 1709 (2015).
- S. Nojima. et al. : Scientific Reports, 7, 9269 (2017).
- S. Ohnuma. et al.: Fish Pathology, 52(2), 96(2017).
- A. Konno and S. Okazaki.: Zoological Letters, 4:13(2018).
- M Kuroda and S Kuroda (2020). Whole-body clearing of beetles by successive treatment of hydrogen peroxide and CUBIC reagents.
- Miyawaki, T. et al. : Nat Commun., 27, 11(2020).
WAKO LabAssay系列试剂盒(比色法)
WAKO LabAssay系列试剂盒(比色法)
Wako LabAssay系列试剂盒是利用实验动物的血清进行生化检验的试剂盒。使用微孔板检测,仅需少量样品,可同时进行多样品检测,检测常规的临床生化指标(脂质,酶,蛋白质,糖)。
品牌 | 产品编号 | 产品名称 | 等级 | 规格 |
Wako 和光纯药 | 633-51021 | LabAssay™ ALP LabAssay™ 碱性磷酸酶检测试剂盒 | – | 500 tests |
Wako 和光纯药 | 632-50991 | LabAssay™ Triglyceride LabAssay™ 甘油三酯检测试剂盒 | – | 350 tests |
Wako 和光纯药 | 638-50971 | LabAssay™ Glucose LabAssay™ 葡萄糖检测试剂盒(Mutarotase-GOD法) | – | 500 tests |
Wako 和光纯药 | 635-50981 | LabAssay™ Cholesterol LabAssay™ 胆固醇检测试剂盒 | – | 500 tests |
Wako 和光纯药 | 639-51001 | LabAssay™ Phospholipid LabAssay™ 磷脂检测试剂盒 | – | 500 tests |
Wako 和光纯药 | 633-52001 | LabAssay™ NEFA LabAssay™ 游离脂肪酸检测试剂盒 | – | 500 tests |
Wako 和光纯药 | 633-51761 | LabAssay™ Ammonia LabAssay™ 氨检测试剂盒 | – | 700 tests |
Wako 和光纯药 | 636-51011 | LabAssay ™ Creatinine LabAssay™ 肌酐检测试剂盒 | – | 500 tests |
Wako 和光纯药 | 290-63701 替代品:632-50991 |
LabAssay™ Triglyceride LabAssay™ 甘油三酯检测试剂盒 | for Cellbiology | 1000 tests |
Wako 和光纯药 | 290-65901 替代品:636-51011 |
LabAssay™ Creatinine LabAssay™ 肌酐检测试剂盒 | for Cellbiology | 500 tests |
Wako 和光纯药 | 291-58601 替代品:633-51021 |
LabAssay™ ALP LabAssay 碱性磷酸酶检测试剂盒 | for Cellbiology | 900 tests |
Wako 和光纯药 | 294-63601 替代品:633-52001 |
LabAssay™ NEFA LabAssay™ 游离脂肪酸检测试剂盒 | for Cellbiology | 750 tests |
Wako 和光纯药 | 294-65801 替代品:635-50981 |
LabAssay™ Cholesterol LabAssay™ 胆固醇检测试剂盒 | for Cellbiology | 1000 tests |
Wako 和光纯药 | 296-63801 替代品:639-51001 |
LabAssay™ Phospholipid LabAssay™ 磷脂检测试剂盒 | for Cellbiology | 1300 tests |
Wako 和光纯药 | 298-65701 替代品:638-50971 |
LabAssay™ Glucose LabAssay™ 葡萄糖检测试剂盒(Mutarotase-GOD法) | for Cellbiology | 1000 tests |
Wako 和光纯药 | 292-63901 | LabAssay™ A/G | for Cellbiology | 1000 tests |
Wako 和光纯药 | 292-64001 | LabAssay™ Uric Acid | for Cellbiology | 1300 tests |
Wako 和光纯药 | 295-78901 替代品:633-51761 |
LabAssay™ Ammonia LabAssay™ 氨检测试剂盒 | for Cellbiology | 700 tests |
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