Wako 043-22351 DA-64

Wako 043-22351 DA-64

货号:043-22351
CAS号:115871-19-7
英文名:DA-64

中文名称:N-(羧甲基氨基羰基)-4,4′-双(二甲氨基)二苯胺钠
保存条件:2-10℃
规格:100mg

2514694138412242.jpg

◆储存条件:2-10℃(冷藏运输)

◆分子式:C19H23N4NaO3

◆分子量:378.39

◆外观

白色~绿褐色或灰绿色,结晶性粉末~粉末(引用文献:和光纯药产品规格)

◆溶解性

可溶于水(10 mg/25 mL)

以1 mM以上的浓度溶于水

◆产品概要

检测波长

λmax(显色波长)= 727 nm,ε/H2O2(一个H2O2分子的消光系数)=9×104

分类

酶·底物·抑制剂>>酶底物>>其他底物

原理

过氧化氢是生物体中痕量组分分析、食品质量监控、环境物质检测等过程的重要中间代谢物。DA-64、DA-67是在有过氧化物酶的存在下高灵敏度地检测出过氧化氢的水溶性氧化显色试剂。传统的水溶性过氧化氢显色试剂由于显色色素的分子吸光系数较低以及虽具有高灵敏度,但是因其微溶性难以处理。DA-64,DA-67首次兼容了水溶性和高灵敏度。以往,为了追求高灵敏度,不得不使用发光、荧光试剂。现在,特殊的专用检测机的检测对象也可通过采用DA-64,DA-67,用简便的比色计就可以获取结果。

◆特点

兼容高灵敏度和水溶性(引用文献:和光纯药情报)

◆使用例

定量H2O2

1. 制备显色溶液

把3.78 mg DA64和1 mL 100 units/mL的过氧化物酶 (Peroxidase,下文简称POD)放到PIPES缓冲液(0.1mol/L, pH=7, Triton X-100 0.5%)中溶解,定容至100 mL(得到DA64 100 μmol/L溶液)

2. 将上述制备的溶液3 mL与10 μL样本溶液(H2O2:0~5 mmol/L)混合,并在37°C下孵育5分钟。

3. 用分光光度计检测727 nm处的吸光度,以空白(用蒸馏水替换样本溶液混合成的溶液)的吸光度差作为检测值,用单独制备的校准曲线确定样本中的含量。

检测POD活性

1. 制备显色溶液

把40 mg DA64和1 mL H2O2(2%)放到Mcllvaine缓冲液(柠檬酸0.05 mol/L、磷酸二钠0.1 mol/L, pH=5.7)中溶解,定容至100 mL(得到DA64 100 mmol/L溶液)

2. 将上述制备的溶液2 mL与2 mL样本溶液(1 fmol~1 pmol/tube POD)在37°C下孵育。

3. 用分光光度计检测727 nm处的吸光度,以减去空白的吸光度差作为检测值,用单独制备的校准曲线确定样本中的含量。

◆用途

过氧化氢比色定量(引用文献:和光纯药情报)

◆产品列表

产品编号 产品名称 等级 包装
043-22351 DA-64 生化学用 100 mg
  • 温馨提示:不可用于临床治疗

193-03065  Pyruvic acid Na 500g

197-02865  Na2HPO4 500g

027-07692  1H-Benzotriazole25g

112-00433  1-Kestose 1g

116-00431  1-Kestose 250mg

205-04196  2,2′-Thiodiethanol 500mL

132-07071  2-Methylisoborneol Standard 20mg

328-33402  2-Mercaptobenzothiazole Zinc Salt 25g

137-16051  3-MCPD Standard 100mg

130-11301  4,4′-Methylenediphenol 5g

196-05375  5mol/l Sodium Hydroxide Solution 500ml

019-11941  Active Carbon-impregnated Silica Gel 10

308-34201  AFLAKING 25

304-34203  AFLAKING 50 50本入

016-11875  Agar (Powder)500g

307-50651  alpha-Glucosidase, from Yeast 100u

139-15911  (Z)-Metominostrobin Standard 20mg

011-13363  Amphotericin B 1g

018-17435  Antifoam SI 500ml

015-14922  Apatite TTCP, monoclinic

609-07511  AQUAMICRONR Check Solution P100ml

016-19131  Artepillin C,from Propolis 10mg

022-00425  Barium Sulfate 500g

027-01276  Benzyl Alcohol 500mL

028-17811  BES-H2O2-Ac 1mg

021-17801  BES-So-AM 1mg

032-17991  beta-Carotene Standard 10mg

309-01401  beta-Sitosterol 100mg

294-31351  BioCleanTip Wako? 1000 1Box

290-31451  BioCleanTip Wako? 200 1Box

026-17415  Bonito Extract

021-02195  Boric Acid 500g

027-03052  Bromothymol Blue 25g

036-00382  Calcium Carbonate 2

031-00435  Calcium Chloride Dihydrate 500g

306-01391  Campesterol 25mg

032-15372  Casein Phosphopeptide 25g

039-12901  Catalase from bovine liver 1g

032-10573  Chloramphenicol 100g

036-10571  Chloramphenicol5g

439-17501  Cholesterol E 100 tests

032-08385  Citric Acid Monohydrate 500g

306-50481  Coenzyme A, Free Acid(CoA) 100mg

638-00781  Collagen Gel Culturing Kit 1Kit

038-04122  Copper(II) Chloride Dihydrate 2

039-06955  Copper, Shot500g

038-22001  Coptisine Chloride 10mg

034-09901  Curdlan 100g

032-09902  Curdlan  25g

073-02792  D(+)-Glucosamine Hydrochloride 25g

049-31165  D(+)-Glucose 試薬特級 500g

070-00482  D(+)-Glucuronolactone 25g

136-00612  D(+)-Maltose Monohydrate  25g

043-22351  DA-64 100mg

044-30451  Diafenthiuron-urea Standard  100mg

043-21991  Difructose Anhydride III  100mg

046-21981  Dimethyl Sulfoxide 100mL

164-04315  Dipotassium Hydrogen Phosphate  500g

049-16787  Distilled Water  20L

352-15085  EcosorbR C-941 500g

392-00471  EC培地EC Medium ”DAIGO” 300g

312-01791  Ethachinmate 0.2ml

079-00614  Glycerol  100ml

060-01081  Fast Violet B Salt 1.5g

060-04881  Flonicamid Standard  200mg

331-31293  Glt-Ala-Ala-Pro-Leu-p-NA.H2O  1g

431-52501  HDL-Cholesterol E 90tests

331-44341  Hepcidin 1 (Mouse) 0.1mg

339-43921  Hepcidin/LEAP-1 (Human) 0.1mg

072-05707  Glucose  10000g

069-00652  Fumaric Acid 25g

396-00511  GITR 500ml*10

070-04821  Glabridin 20mg

097-02471  INDOMETHACIN  10G

085-09291  Hirsutine  5mg

092-05422  Iodine  25g

339-30253  Leu-Gly-Gly  1g

122-03631  Lithium L-Lactate 100g

120-03632  Lithium L-Lactate 25g

138-07512  L-Malic Acid  25g

397-00281  LP稀释液  30g×5

034-06363  Octanoic Acid  25ml

639-09091  PANOSE  1g

050-08121  pEBMulti-Hyg  20ug

135-15011  Menbutone Standard  200mg

056-00242  meso-Erythritol  25g

131-02862  METHYL ORANGE  25g

534-02315  Hyflo Super-Cel 500g

098-01085  Iron(II) Sulfate Heptahydrate 500g

152-00373  L(+)-Ornithine Monohydrochloride 100g

010-18931  L-2-Aminooxy-3-phenylpropionic Acid 50mg

294-65801  LabAssay  Cholesterol 1000回用

296-63801  LabAssay Phospholipid  1300tests

290-63701  LabAssay  Triglyceride 1000回用

123-04124  Lactoferrin, from Bovine Milk 1g

128-00095  Lactose Monohydrate  500g

037-05275  L-Cysteine Hydrochloride Monohydrate 500g

127-04301  Lead Standard Solution (Pb 100) 100ml

299-51201  LES-2T (LIMULUS ES-2) 60tests

337-30911  Leu-MCA[Tosylate Form] 5mg

292-32751  Limulus Test Tube-S with Aluminum Cap

325-88521  Lipase PS Amano SD  10g

131-16571  Mogroside V Standard  50mg

129-02541  Lysyl EndopeptidaseR  1vial (10AU)

125-02543  Lysyl EndopeptidaseR  1vial (2AU)

013-04815  L-ASPARAGINE, MONOHYDRATE 500g

131-00405  Magnesium Sulfate Heptahydrate 500g

130-07773  Maltohexaose 500mg

134-07433  Maltopentaose 1g

139-00722  Manganese(II) Chloride Tetrahydrate 25g

605-07471  MCI Buffer L-8500-PF Kit  1kit

639-12421   MCI Buffer L-8500-PF-1 2L

608-07461  MCI Buffer L-8500-PH Kit  1kit

604-07463  MCI Buffer L-8500-PH-1  10L

634-12471  MCI Buffer L-8500-PH-1  1L×2

631-12481  MCI Buffer L-8500-PH-2  1L×2

606-07462  MCI Buffer L-8500-PH-3

638-12491  MCI Buffer L-8500-PH-3  2L

602-07464  MCI Buffer L-8500-PH-4

631-12501  MCI Buffer L-8500-PH-4  2L

600-07465  MCI Buffer L-8500-PH-RG  1L×10

133-14331  Menaquinone-7 Standard  20mg

143-05983  Nystose Trihydrate  1g

147-03781  N-ニトロソジメチルアミン標準品  1g

012-07021  O-Acetyl-beta-methylcholine Chloride  10g

168-23191  Penicillin-Streptomycin Solution ( x 100)  100ml

195-03645  Pentasodium Triphosphate  500g

162-18101  Peptidoglycan, from Micrococcus luteus  2mL

161-24161  Perillaldehyde   30mg×5A

300-93523  Phos-tag (TM) Acrylamide AAL-107  2mg/瓶

301-93531  Phos-tag (TM) Biotin BTL-104  10mg

304-93521  Phos-tag  AAL-107  10mg

163-11051  Polyamide C-100  200g

164-08531  Polyamide FM Plate  10枚

161-09065  Polyethylene Glycol 400  500g

169-09125  POLYETHYLENE GLYCOL 6,000  500G

166-21115  Polyoxyethylene(20) Sorbitan Monolaurate 500g

162-11565  Polyoxyethylene(23) Lauryl Ether 500g

392-02115  Polypepton 500g

165-17035  Polyvinylpyrrolidone K 30   500g

169-04245  Potassium Dihydrogen Phosphate  500g

160-04275  Potassium Dihydrogen Phosphate  500g

167-03722  Potassium Hexacyanoferrate(III)  25g

162-03071  Potassium Polyvinyl Sulfate  10g

290-34251  Presep-C DNPH  20個

018-00943  Ribitol  5g

398-00255  SCDLP寒天培地  500g

395-00265  SCDLP培地 , 一般細菌検査用  500g

393-00185  SCDLP培地 , 一般細菌検査用  500g

396-00175  SCD寒天培地 , 一般細菌検査用  500g

194-16241  SENNOSIDE A  10mg

192-09071  Silicon Dioxide, 99.9%  100g

192-06031  Silicon Standard Solution  100ml

194-00832  Silver Nitrate  25g

196-12781  Simeconazole Standar  200mg

297-51501  SLP Reagent Set  3mL用

293-58301  SLP-HS Single Reagent Set  20回用

198-01055  Sodium Acetate Trihydrate  500g

194-13321  Sodium Alginate 80-120  100g

198-01675  Sodium Chloride  500g

199-08361  Sodium Dextran Sulfate 5,000

194-13402  Sodium Dextran Sulfate 5000  25g

197-09705  Sodium Dihydrogenphosphate  500g

192-02815  Sodium Dihydrogenphosphate Dihydrate  500g

190-02811  Sodium Dihydrogenphosphate Dihydrate  100g

192-07935  Sodium Hydroxide  500g

197-03345  Sodium Sulfate  500g

197-07125  Sodium Sulfate  500g

191-03985  Soluble Starch 和光一級  500g

199-16291  Stevioside Standard  100mg

196-00015  Sucrose  500g

191-04502  Sulfanilamide  25g

204-08343  Testosterone  5g

203-00432  Tetraethylammonium Bromide  25g

205-00431  Tetraethylammonium Bromide  100g

208-16771  TFNG Standard  20mg

124-02275  Trilithium Citrate Tetrahydrate  500g

206-15591  Trinexapac Standard  200mg

190-05535  Trisodium Citrate Dihydrate  500g

208-09223  Trypsin Inhibitor, from Soybean  1g

028-03386  t-Butyl Alcohol 500ml

223-02112  VA-044  25G

226-01306  Vancomycin Hydrochloride  5g

302-07111  Vitamin E Reference Standard  1kit

234-01565  WakogelR 50C18  500g

396-01731  XLD 日局試験用  300g

035-17981  α-Carotene Standard  10mg

354-03232  γ-Phenylpropyl Acetate  25g

242-00641  Xylobiose  100mg

334-30183  Z-Glu-Tyr  1g

263-00271  Zinc Chloride  10g

033-08332  α-Cyclodextrin  25g

034-13632  Chitin  25g

030-09903  Curdlan  1g

342-01875  EDTA-3NA  500g

030-05525  Citric Acid  500g

999-09801  游离脂肪酸  2*19ml

995-09901  游离脂肪酸  2*10ml

635-25711  GIT?  500ml×10

638-01021  endotoxin standard/jpse 10000UN

030-01505Casein, from Milk  500g

195-07925  Sucrose  500g

191-01785   柠檬酸三钠二水合物 试剂特级  500g

191-01785  Trisodium Citrate, Dihydrate  50ml

077-00735 甘氨酸 Glycine  500g

075-00616  丙三醇(甘油) Glycerol  500ml

034-14612  Chondroitin Sulfate C Sodium Salt  25g

035-16521  Cinmethylin Standard  200mg

307-33811  SmartAssay Iprodione Test Kit  96test

399-01721  Cetrimide agar DAIGO for JP general test  300g

205-08591  Tetracycline Hydrochloride  5G

195-03961  Starch, Soluble  100g

141-01202  Nicotinamide  25g

145-08221 核酸酶P1  nuclease p1  500U

395-01181  Vogel-Johnson Agar ”DAIGO”  300g

197-02206  Sodium Hypochlorite Solution  500ml

094-05421 碘测试剂  100g

398-02151  D-3H DRIED YEAST EXTRACT D-3H  250g

010-15815  Agar Powder寒天特級  500g

018-08721  AGAR,POWDER琼脂粉  100g

010-08725  寒天,粉末   細菌培地用 500g

165-08245  精制水  500ml

011-23731  硫酸阿托品水合物测试剂  50MG

096-01081  硫酸鉄七水和物  100g

299-70501  日立用茚三酮显色液  2L

391-01041  Lactose Broth  300g

199-01585  炭酸ナトリウム Sodium Carbonate  500g

197-01307  炭酸水素????? NaHCO3  15000g

191-01305   Sodium Hydrogen Carbonate  500g

636-15133  Collagenase L  100mg

161-03541  Potassium Chloride  4000g

169-03547  Potassium Chloride  KCl  20kg

039-00475  Calcium Chloride  500g

197-01667  Sodium Chloride  20kg

191-01665  Sodium Chloride  500g

135-00165  Magnesium Chloride Hexahydrate  500g

125-02445  Lithium Chloride  500g

060-01802  叶酸 Folic acid  25g

276-76491  遊離脂肪酸基準液  10mL×4

295-73401  Fructooligosaccharides Set  1kit

293-40351  Presep-C Ozone Scrubber  20個

291-43951  DNPH预处理小柱小分子醛酮的检测  20入

023-07731  Benzylpenicillin Potassium  5G

205-08775  Carbonate pH Standard Solution  500ml

161-17032  Polyvinylpyrrolidone K 30  25g

164-21655  聚乙烯硫酸钾滴定液(N/400)  500ml

390-01751  MacConkey agar DAIGO for JP general test  300g

391-01801  MacConkey broth DAIGO for JP general test  300g

135-17711  Manninotriose甘露三糖  10mg

136-03032  Methyl Red  25g

129-04121  Lactoferrin, from Bovine Milk  5g

294-63601  NEFA kit  750回用

012-03305  Ammonium Dihydrogenphosphate  500g

164-04295  Dipotassium Hydrogenphosphate  500g

232-50891  Wakopak Wakosil-II5C18AR Prep 4.6*250mm  1本(D)

294-64701  Human/Rat βAmyloid(40)ELISA Kit Wako Ⅱ 96回用

165-12155  Phosphate pH Standard Equimolal Solution  500ml

072-04141  β-D-Galactosidase, from Escherichia coli  10000UN

308-97201  Phos-tag Biotin BTL-111 1mM Aqueous Solution  0.1mL

290-62601  Human/Rat β Amyloid(42)ELISA Kit Wako 96Tests

160-03055  Polyvinyl Alcohol (Polymerization Degree about 1500) 500g

123-02505  pH 2.2 Lithium Citrate Buffer Solution  500mL

195-07165  pH 2.2 Sodium Citrate Buffer Solution  500ml

125-05061  Lysyl Endopeptidase, Mass Spectrometry Grade 20μg×5

230-63381  Wakopak Fluofix-Ⅱ 120E 4.6*250mm  1本(D)

232-50151  Wakopak Wakosil-II3C18AR 4.6*50mm  1本(D)

045-29511  N1,N12-Diacetylspermin n-Hydrochloride  40mg

052-06121  N-Ethyl-N-isothiocyanatobutyl Isoluminol  10mg

343-02042  NN-希釈粉末/NN diluted with Potassium Sulfate  25g

231-50361  Wakopak Wakosil-II3C18HG 4.6*100mm  1本(D)

238-50773  Wakopak Wakosil-II5C18AR 4.6*250mm  1Column(W)

190-05395  0.2mol/l Sodium Hydroxide Solution 500ml

197-11611  10% Silver Nitrate-impregnated Silica Gel 100g

234-51113  Wakopak Wakosil-II5C18HG 4.6*150mm  1Column(W)

238-51471  Wakopak Wakosil-II5C18RS 4.6*150mm  1Column(D)

238-01921  Wetting Tension Test Mixture No.40.0  50ml

235-01931  Wetting Tension Test Mixture No.41.0  50ml

263-00891  Zirconium Standard Solution (Zr 1000ppm)  100mL

232-01941  Wetting Tension Test Mixture No.42.0  50mL

236-01961  Wetting Tension Test Mixture No.44.0  50ml

230-01981  Wetting Tension Test Mixture No.46.0  50ml

019-19741  Anti Iba1, Rabbit (for Immunocytochemistry) 50ug

016-20001  Anti Iba1, Rabbit (for Western Blotting) 50ug

341-01622  2-Morpholinoethanesulfonic acid, monohydrate 25G

199-07065  0.0125mol/l  Sodium Oxalate Solution 500ml

161-07505  0.1mol/l Perchloric Acid (Acetic Acid Solution) 500ml

035-21891  3-Chloro-1,2-propanediol Dilinoleate Standard 100mg

031-21511  3-Chloro-1,2-propanediol Dioleate Standard 100m

037-21471  3-Chloro-1,2-propanediol Dipalmitate Standard 100mg

038-21881  3-Chloro-1,2-propanediol Distearate Standard 100mg

084-07821  (-)-Hydroxycitric Acid Calcium Salt Standard 200mg

017-18321  (R)-(-)-Apomorphine Hydrochloride Hemihydrate 100mg

012-18011  40% Alkyldiaminoethylglycine Hydrochloride Solution 250mL

237-01991  Wetting Tension Test Mixture No.48.0  50ml

234-02001  Wetting Tension Test Mixture No.50.0  50mL

037-01032  D-Camphor-10-sulfonic Acid Sodium Salt 2

342-02615  EDTA.2Na solution(0.01mol/l) T013 500ml

057-02195  Ethyl Cellulose (abt.49% ethoxy) 10  500g

031-16741  Cycloartenyl Ferulate Standard 100mg

053-00735  Ethyl Cellulose (abt.49% ethoxy) 45  500g

050-06041  Eosin Alcohol Solution, acid extract 1L

231-02011  Wetting Tension Test Mixture No.52.0  50ml

238-02021  Wetting Tension Test Mixture No.54.0  50mL

235-02031  Wetting Tension Test Mixture No.56.0  50mL

239-02051  Wetting Tension Test Mixture No.59.0  50mL

399-01081  Tetrathionate Broth DAIGO, for JP General Test  300g

033-23271  Casein, from Bovine Milk (acc. to Hammarsten)  100g

236-02061  Wetting Tension Test Mixture No.60.0  50ml

233-02071  Wetting Tension Test Mixture No.61.0  50mL

230-02081  Wetting Tension Test Mixture No.62.0  50ml

231-02131  Wetting Tension Test Mixture No.70.0  50ml

238-02141  Wetting Tension Test Mixture No.73.0  50mL

631-00651  Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 20ml

396-01851  Mannitol salt agar DAIGO for JP general test 300g

094-05301  Isometamidium Chloride Standard(mixture of isomers) 200mg

326-91202  Lipase PS IM Amano,Immobilized on Diatomaceous Earth  25g

168-17461  Phosphate Ion Standard Solution (PO4(3-) : 1,000)  50ml

302-50581  Lactate Dehydrogenase, from Rabbit Muscle (LDH (R.M.)) 10000units

399-01841  Potato dextrose agar DAIGO for JP general test  300g

161-14695  0.0025N Poly(diallyldimethylammonium Chloride) Solution 500ml

399-01101  Brilliant Green Agar DAIGO, for JP General Test  300g

392-01831  Sabouraud-dextrose agar DAIGO for JP general test  300g

167-21341  フタル酸水素カリウム標準液 (Cとして : 1mg/ml 水溶液)  50ml

390-01131  Triple Sugar-Iron Agar DAIGO, for JP General Test 300g

146-08251 呋喃苯烯酸钠标准品Sodium Nifurstyrenate Standard  200mg

394-01771  Casein soya bean digest agar DAIGO for JP general test  300g

304-93526  Phos-tag (TM) Acrylamide AAL-107 5mM Aqueous Solution  0.3mL

395-01061   EMB寒天培地 , 日局試験用EMB Agar,”DAIGO”, for JP General Test 300g

394-00235   GPLP寒天培地?Daigo Glucose Peptone Agar With Lecithin & Polysorbate 80. 500g

345-01865   Ethylenediamine-N,N,N’,N’-tetraacetic Acid Disodium Salt Dihydrate (2NA) 500g

392-01875  Sabouraud-Dextrose Agar with Lecithin & Polysorbate 80 ”DAIGO”  500g

399-01885  Sabouraud-Dextrose Broth with Lecithin & Polysorbate 80 ”DAIGO’  500g

025-03195  Buffer Solution Standard (Phosphate pH Standard Equimolal Solution) pH6.86 (25 degrees C) 500ml

028-03185 Buffer Solution Standard (Phthalate pH Standard Solution) pH4.01 (25 degrees C) 500ml

028-03205  Buffer Solution Standard (Tetraborate pH Standard Solution) pH9.18 (25 degrees C) 500ml

168-12145  Potassium Hydrogen Phthalate Standard Solution (as C:1mg/ml Water Solution)  500ml

392-01711  Buffered sodium chloride-peptone solution pH 7.0 DAIGO for JP general test 300g

393-01741  Rappaport Vassiliadis Salmonella Enrichment Broth DAIGO for JP general test  300g