Wako 043-22351 DA-64
货号:043-22351
CAS号:115871-19-7
英文名:DA-64
中文名称:N-(羧甲基氨基羰基)-4,4′-双(二甲氨基)二苯胺钠
保存条件:2-10℃
规格:100mg
◆储存条件:2-10℃(冷藏运输)
◆分子式:C19H23N4NaO3
◆分子量:378.39
◆外观
白色~绿褐色或灰绿色,结晶性粉末~粉末(引用文献:和光纯药产品规格)
◆溶解性
可溶于水(10 mg/25 mL)
以1 mM以上的浓度溶于水
◆产品概要
检测波长
λmax(显色波长)= 727 nm,ε/H2O2(一个H2O2分子的消光系数)=9×104
分类
酶·底物·抑制剂>>酶底物>>其他底物
原理
过氧化氢是生物体中痕量组分分析、食品质量监控、环境物质检测等过程的重要中间代谢物。DA-64、DA-67是在有过氧化物酶的存在下高灵敏度地检测出过氧化氢的水溶性氧化显色试剂。传统的水溶性过氧化氢显色试剂由于显色色素的分子吸光系数较低以及虽具有高灵敏度,但是因其微溶性难以处理。DA-64,DA-67首次兼容了水溶性和高灵敏度。以往,为了追求高灵敏度,不得不使用发光、荧光试剂。现在,特殊的专用检测机的检测对象也可通过采用DA-64,DA-67,用简便的比色计就可以获取结果。
◆特点
兼容高灵敏度和水溶性(引用文献:和光纯药情报)
◆使用例
定量H2O2
1. 制备显色溶液
把3.78 mg DA64和1 mL 100 units/mL的过氧化物酶 (Peroxidase,下文简称POD)放到PIPES缓冲液(0.1mol/L, pH=7, Triton X-100 0.5%)中溶解,定容至100 mL(得到DA64 100 μmol/L溶液)
2. 将上述制备的溶液3 mL与10 μL样本溶液(H2O2:0~5 mmol/L)混合,并在37°C下孵育5分钟。
3. 用分光光度计检测727 nm处的吸光度,以空白(用蒸馏水替换样本溶液混合成的溶液)的吸光度差作为检测值,用单独制备的校准曲线确定样本中的含量。
检测POD活性
1. 制备显色溶液
把40 mg DA64和1 mL H2O2(2%)放到Mcllvaine缓冲液(柠檬酸0.05 mol/L、磷酸二钠0.1 mol/L, pH=5.7)中溶解,定容至100 mL(得到DA64 100 mmol/L溶液)
2. 将上述制备的溶液2 mL与2 mL样本溶液(1 fmol~1 pmol/tube POD)在37°C下孵育。
3. 用分光光度计检测727 nm处的吸光度,以减去空白的吸光度差作为检测值,用单独制备的校准曲线确定样本中的含量。
◆用途
过氧化氢比色定量(引用文献:和光纯药情报)
◆产品列表
产品编号 | 产品名称 | 等级 | 包装 |
043-22351 | DA-64 | 生化学用 | 100 mg |
-
温馨提示:不可用于临床治疗
193-03065 Pyruvic acid Na 500g
197-02865 Na2HPO4 500g
027-07692 1H-Benzotriazole25g
112-00433 1-Kestose 1g
116-00431 1-Kestose 250mg
205-04196 2,2′-Thiodiethanol 500mL
132-07071 2-Methylisoborneol Standard 20mg
328-33402 2-Mercaptobenzothiazole Zinc Salt 25g
137-16051 3-MCPD Standard 100mg
130-11301 4,4′-Methylenediphenol 5g
196-05375 5mol/l Sodium Hydroxide Solution 500ml
019-11941 Active Carbon-impregnated Silica Gel 10
308-34201 AFLAKING 25
304-34203 AFLAKING 50 50本入
016-11875 Agar (Powder)500g
307-50651 alpha-Glucosidase, from Yeast 100u
139-15911 (Z)-Metominostrobin Standard 20mg
011-13363 Amphotericin B 1g
018-17435 Antifoam SI 500ml
015-14922 Apatite TTCP, monoclinic
609-07511 AQUAMICRONR Check Solution P100ml
016-19131 Artepillin C,from Propolis 10mg
022-00425 Barium Sulfate 500g
027-01276 Benzyl Alcohol 500mL
028-17811 BES-H2O2-Ac 1mg
021-17801 BES-So-AM 1mg
032-17991 beta-Carotene Standard 10mg
309-01401 beta-Sitosterol 100mg
294-31351 BioCleanTip Wako? 1000 1Box
290-31451 BioCleanTip Wako? 200 1Box
026-17415 Bonito Extract
021-02195 Boric Acid 500g
027-03052 Bromothymol Blue 25g
036-00382 Calcium Carbonate 2
031-00435 Calcium Chloride Dihydrate 500g
306-01391 Campesterol 25mg
032-15372 Casein Phosphopeptide 25g
039-12901 Catalase from bovine liver 1g
032-10573 Chloramphenicol 100g
036-10571 Chloramphenicol5g
439-17501 Cholesterol E 100 tests
032-08385 Citric Acid Monohydrate 500g
306-50481 Coenzyme A, Free Acid(CoA) 100mg
638-00781 Collagen Gel Culturing Kit 1Kit
038-04122 Copper(II) Chloride Dihydrate 2
039-06955 Copper, Shot500g
038-22001 Coptisine Chloride 10mg
034-09901 Curdlan 100g
032-09902 Curdlan 25g
073-02792 D(+)-Glucosamine Hydrochloride 25g
049-31165 D(+)-Glucose 試薬特級 500g
070-00482 D(+)-Glucuronolactone 25g
136-00612 D(+)-Maltose Monohydrate 25g
043-22351 DA-64 100mg
044-30451 Diafenthiuron-urea Standard 100mg
043-21991 Difructose Anhydride III 100mg
046-21981 Dimethyl Sulfoxide 100mL
164-04315 Dipotassium Hydrogen Phosphate 500g
049-16787 Distilled Water 20L
352-15085 EcosorbR C-941 500g
392-00471 EC培地EC Medium ”DAIGO” 300g
312-01791 Ethachinmate 0.2ml
079-00614 Glycerol 100ml
060-01081 Fast Violet B Salt 1.5g
060-04881 Flonicamid Standard 200mg
331-31293 Glt-Ala-Ala-Pro-Leu-p-NA.H2O 1g
431-52501 HDL-Cholesterol E 90tests
331-44341 Hepcidin 1 (Mouse) 0.1mg
339-43921 Hepcidin/LEAP-1 (Human) 0.1mg
072-05707 Glucose 10000g
069-00652 Fumaric Acid 25g
396-00511 GITR 500ml*10
070-04821 Glabridin 20mg
097-02471 INDOMETHACIN 10G
085-09291 Hirsutine 5mg
092-05422 Iodine 25g
339-30253 Leu-Gly-Gly 1g
122-03631 Lithium L-Lactate 100g
120-03632 Lithium L-Lactate 25g
138-07512 L-Malic Acid 25g
397-00281 LP稀释液 30g×5
034-06363 Octanoic Acid 25ml
639-09091 PANOSE 1g
050-08121 pEBMulti-Hyg 20ug
135-15011 Menbutone Standard 200mg
056-00242 meso-Erythritol 25g
131-02862 METHYL ORANGE 25g
534-02315 Hyflo Super-Cel 500g
098-01085 Iron(II) Sulfate Heptahydrate 500g
152-00373 L(+)-Ornithine Monohydrochloride 100g
010-18931 L-2-Aminooxy-3-phenylpropionic Acid 50mg
294-65801 LabAssay Cholesterol 1000回用
296-63801 LabAssay Phospholipid 1300tests
290-63701 LabAssay Triglyceride 1000回用
123-04124 Lactoferrin, from Bovine Milk 1g
128-00095 Lactose Monohydrate 500g
037-05275 L-Cysteine Hydrochloride Monohydrate 500g
127-04301 Lead Standard Solution (Pb 100) 100ml
299-51201 LES-2T (LIMULUS ES-2) 60tests
337-30911 Leu-MCA[Tosylate Form] 5mg
292-32751 Limulus Test Tube-S with Aluminum Cap
325-88521 Lipase PS Amano SD 10g
131-16571 Mogroside V Standard 50mg
129-02541 Lysyl EndopeptidaseR 1vial (10AU)
125-02543 Lysyl EndopeptidaseR 1vial (2AU)
013-04815 L-ASPARAGINE, MONOHYDRATE 500g
131-00405 Magnesium Sulfate Heptahydrate 500g
130-07773 Maltohexaose 500mg
134-07433 Maltopentaose 1g
139-00722 Manganese(II) Chloride Tetrahydrate 25g
605-07471 MCI Buffer L-8500-PF Kit 1kit
639-12421 MCI Buffer L-8500-PF-1 2L
608-07461 MCI Buffer L-8500-PH Kit 1kit
604-07463 MCI Buffer L-8500-PH-1 10L
634-12471 MCI Buffer L-8500-PH-1 1L×2
631-12481 MCI Buffer L-8500-PH-2 1L×2
606-07462 MCI Buffer L-8500-PH-3
638-12491 MCI Buffer L-8500-PH-3 2L
602-07464 MCI Buffer L-8500-PH-4
631-12501 MCI Buffer L-8500-PH-4 2L
600-07465 MCI Buffer L-8500-PH-RG 1L×10
133-14331 Menaquinone-7 Standard 20mg
143-05983 Nystose Trihydrate 1g
147-03781 N-ニトロソジメチルアミン標準品 1g
012-07021 O-Acetyl-beta-methylcholine Chloride 10g
168-23191 Penicillin-Streptomycin Solution ( x 100) 100ml
195-03645 Pentasodium Triphosphate 500g
162-18101 Peptidoglycan, from Micrococcus luteus 2mL
161-24161 Perillaldehyde 30mg×5A
300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg/瓶
301-93531 Phos-tag (TM) Biotin BTL-104 10mg
304-93521 Phos-tag AAL-107 10mg
163-11051 Polyamide C-100 200g
164-08531 Polyamide FM Plate 10枚
161-09065 Polyethylene Glycol 400 500g
169-09125 POLYETHYLENE GLYCOL 6,000 500G
166-21115 Polyoxyethylene(20) Sorbitan Monolaurate 500g
162-11565 Polyoxyethylene(23) Lauryl Ether 500g
392-02115 Polypepton 500g
165-17035 Polyvinylpyrrolidone K 30 500g
169-04245 Potassium Dihydrogen Phosphate 500g
160-04275 Potassium Dihydrogen Phosphate 500g
167-03722 Potassium Hexacyanoferrate(III) 25g
162-03071 Potassium Polyvinyl Sulfate 10g
290-34251 Presep-C DNPH 20個
018-00943 Ribitol 5g
398-00255 SCDLP寒天培地 500g
395-00265 SCDLP培地 , 一般細菌検査用 500g
393-00185 SCDLP培地 , 一般細菌検査用 500g
396-00175 SCD寒天培地 , 一般細菌検査用 500g
194-16241 SENNOSIDE A 10mg
192-09071 Silicon Dioxide, 99.9% 100g
192-06031 Silicon Standard Solution 100ml
194-00832 Silver Nitrate 25g
196-12781 Simeconazole Standar 200mg
297-51501 SLP Reagent Set 3mL用
293-58301 SLP-HS Single Reagent Set 20回用
198-01055 Sodium Acetate Trihydrate 500g
194-13321 Sodium Alginate 80-120 100g
198-01675 Sodium Chloride 500g
199-08361 Sodium Dextran Sulfate 5,000
194-13402 Sodium Dextran Sulfate 5000 25g
197-09705 Sodium Dihydrogenphosphate 500g
192-02815 Sodium Dihydrogenphosphate Dihydrate 500g
190-02811 Sodium Dihydrogenphosphate Dihydrate 100g
192-07935 Sodium Hydroxide 500g
197-03345 Sodium Sulfate 500g
197-07125 Sodium Sulfate 500g
191-03985 Soluble Starch 和光一級 500g
199-16291 Stevioside Standard 100mg
196-00015 Sucrose 500g
191-04502 Sulfanilamide 25g
204-08343 Testosterone 5g
203-00432 Tetraethylammonium Bromide 25g
205-00431 Tetraethylammonium Bromide 100g
208-16771 TFNG Standard 20mg
124-02275 Trilithium Citrate Tetrahydrate 500g
206-15591 Trinexapac Standard 200mg
190-05535 Trisodium Citrate Dihydrate 500g
208-09223 Trypsin Inhibitor, from Soybean 1g
028-03386 t-Butyl Alcohol 500ml
223-02112 VA-044 25G
226-01306 Vancomycin Hydrochloride 5g
302-07111 Vitamin E Reference Standard 1kit
234-01565 WakogelR 50C18 500g
396-01731 XLD 日局試験用 300g
035-17981 α-Carotene Standard 10mg
354-03232 γ-Phenylpropyl Acetate 25g
242-00641 Xylobiose 100mg
334-30183 Z-Glu-Tyr 1g
263-00271 Zinc Chloride 10g
033-08332 α-Cyclodextrin 25g
034-13632 Chitin 25g
030-09903 Curdlan 1g
342-01875 EDTA-3NA 500g
030-05525 Citric Acid 500g
999-09801 游离脂肪酸 2*19ml
995-09901 游离脂肪酸 2*10ml
635-25711 GIT? 500ml×10
638-01021 endotoxin standard/jpse 10000UN
030-01505Casein, from Milk 500g
195-07925 Sucrose 500g
191-01785 柠檬酸三钠二水合物 试剂特级 500g
191-01785 Trisodium Citrate, Dihydrate 50ml
077-00735 甘氨酸 Glycine 500g
075-00616 丙三醇(甘油) Glycerol 500ml
034-14612 Chondroitin Sulfate C Sodium Salt 25g
035-16521 Cinmethylin Standard 200mg
307-33811 SmartAssay Iprodione Test Kit 96test
399-01721 Cetrimide agar DAIGO for JP general test 300g
205-08591 Tetracycline Hydrochloride 5G
195-03961 Starch, Soluble 100g
141-01202 Nicotinamide 25g
145-08221 核酸酶P1 nuclease p1 500U
395-01181 Vogel-Johnson Agar ”DAIGO” 300g
197-02206 Sodium Hypochlorite Solution 500ml
094-05421 碘测试剂 100g
398-02151 D-3H DRIED YEAST EXTRACT D-3H 250g
010-15815 Agar Powder寒天特級 500g
018-08721 AGAR,POWDER琼脂粉 100g
010-08725 寒天,粉末 細菌培地用 500g
165-08245 精制水 500ml
011-23731 硫酸阿托品水合物测试剂 50MG
096-01081 硫酸鉄七水和物 100g
299-70501 日立用茚三酮显色液 2L
391-01041 Lactose Broth 300g
199-01585 炭酸ナトリウム Sodium Carbonate 500g
197-01307 炭酸水素????? NaHCO3 15000g
191-01305 Sodium Hydrogen Carbonate 500g
636-15133 Collagenase L 100mg
161-03541 Potassium Chloride 4000g
169-03547 Potassium Chloride KCl 20kg
039-00475 Calcium Chloride 500g
197-01667 Sodium Chloride 20kg
191-01665 Sodium Chloride 500g
135-00165 Magnesium Chloride Hexahydrate 500g
125-02445 Lithium Chloride 500g
060-01802 叶酸 Folic acid 25g
276-76491 遊離脂肪酸基準液 10mL×4
295-73401 Fructooligosaccharides Set 1kit
293-40351 Presep-C Ozone Scrubber 20個
291-43951 DNPH预处理小柱小分子醛酮的检测 20入
023-07731 Benzylpenicillin Potassium 5G
205-08775 Carbonate pH Standard Solution 500ml
161-17032 Polyvinylpyrrolidone K 30 25g
164-21655 聚乙烯硫酸钾滴定液(N/400) 500ml
390-01751 MacConkey agar DAIGO for JP general test 300g
391-01801 MacConkey broth DAIGO for JP general test 300g
135-17711 Manninotriose甘露三糖 10mg
136-03032 Methyl Red 25g
129-04121 Lactoferrin, from Bovine Milk 5g
294-63601 NEFA kit 750回用
012-03305 Ammonium Dihydrogenphosphate 500g
164-04295 Dipotassium Hydrogenphosphate 500g
232-50891 Wakopak Wakosil-II5C18AR Prep 4.6*250mm 1本(D)
294-64701 Human/Rat βAmyloid(40)ELISA Kit Wako Ⅱ 96回用
165-12155 Phosphate pH Standard Equimolal Solution 500ml
072-04141 β-D-Galactosidase, from Escherichia coli 10000UN
308-97201 Phos-tag Biotin BTL-111 1mM Aqueous Solution 0.1mL
290-62601 Human/Rat β Amyloid(42)ELISA Kit Wako 96Tests
160-03055 Polyvinyl Alcohol (Polymerization Degree about 1500) 500g
123-02505 pH 2.2 Lithium Citrate Buffer Solution 500mL
195-07165 pH 2.2 Sodium Citrate Buffer Solution 500ml
125-05061 Lysyl Endopeptidase, Mass Spectrometry Grade 20μg×5
230-63381 Wakopak Fluofix-Ⅱ 120E 4.6*250mm 1本(D)
232-50151 Wakopak Wakosil-II3C18AR 4.6*50mm 1本(D)
045-29511 N1,N12-Diacetylspermin n-Hydrochloride 40mg
052-06121 N-Ethyl-N-isothiocyanatobutyl Isoluminol 10mg
343-02042 NN-希釈粉末/NN diluted with Potassium Sulfate 25g
231-50361 Wakopak Wakosil-II3C18HG 4.6*100mm 1本(D)
238-50773 Wakopak Wakosil-II5C18AR 4.6*250mm 1Column(W)
190-05395 0.2mol/l Sodium Hydroxide Solution 500ml
197-11611 10% Silver Nitrate-impregnated Silica Gel 100g
234-51113 Wakopak Wakosil-II5C18HG 4.6*150mm 1Column(W)
238-51471 Wakopak Wakosil-II5C18RS 4.6*150mm 1Column(D)
238-01921 Wetting Tension Test Mixture No.40.0 50ml
235-01931 Wetting Tension Test Mixture No.41.0 50ml
263-00891 Zirconium Standard Solution (Zr 1000ppm) 100mL
232-01941 Wetting Tension Test Mixture No.42.0 50mL
236-01961 Wetting Tension Test Mixture No.44.0 50ml
230-01981 Wetting Tension Test Mixture No.46.0 50ml
019-19741 Anti Iba1, Rabbit (for Immunocytochemistry) 50ug
016-20001 Anti Iba1, Rabbit (for Western Blotting) 50ug
341-01622 2-Morpholinoethanesulfonic acid, monohydrate 25G
199-07065 0.0125mol/l Sodium Oxalate Solution 500ml
161-07505 0.1mol/l Perchloric Acid (Acetic Acid Solution) 500ml
035-21891 3-Chloro-1,2-propanediol Dilinoleate Standard 100mg
031-21511 3-Chloro-1,2-propanediol Dioleate Standard 100m
037-21471 3-Chloro-1,2-propanediol Dipalmitate Standard 100mg
038-21881 3-Chloro-1,2-propanediol Distearate Standard 100mg
084-07821 (-)-Hydroxycitric Acid Calcium Salt Standard 200mg
017-18321 (R)-(-)-Apomorphine Hydrochloride Hemihydrate 100mg
012-18011 40% Alkyldiaminoethylglycine Hydrochloride Solution 250mL
237-01991 Wetting Tension Test Mixture No.48.0 50ml
234-02001 Wetting Tension Test Mixture No.50.0 50mL
037-01032 D-Camphor-10-sulfonic Acid Sodium Salt 2
342-02615 EDTA.2Na solution(0.01mol/l) T013 500ml
057-02195 Ethyl Cellulose (abt.49% ethoxy) 10 500g
031-16741 Cycloartenyl Ferulate Standard 100mg
053-00735 Ethyl Cellulose (abt.49% ethoxy) 45 500g
050-06041 Eosin Alcohol Solution, acid extract 1L
231-02011 Wetting Tension Test Mixture No.52.0 50ml
238-02021 Wetting Tension Test Mixture No.54.0 50mL
235-02031 Wetting Tension Test Mixture No.56.0 50mL
239-02051 Wetting Tension Test Mixture No.59.0 50mL
399-01081 Tetrathionate Broth DAIGO, for JP General Test 300g
033-23271 Casein, from Bovine Milk (acc. to Hammarsten) 100g
236-02061 Wetting Tension Test Mixture No.60.0 50ml
233-02071 Wetting Tension Test Mixture No.61.0 50mL
230-02081 Wetting Tension Test Mixture No.62.0 50ml
231-02131 Wetting Tension Test Mixture No.70.0 50ml
238-02141 Wetting Tension Test Mixture No.73.0 50mL
631-00651 Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0) 20ml
396-01851 Mannitol salt agar DAIGO for JP general test 300g
094-05301 Isometamidium Chloride Standard(mixture of isomers) 200mg
326-91202 Lipase PS IM Amano,Immobilized on Diatomaceous Earth 25g
168-17461 Phosphate Ion Standard Solution (PO4(3-) : 1,000) 50ml
302-50581 Lactate Dehydrogenase, from Rabbit Muscle (LDH (R.M.)) 10000units
399-01841 Potato dextrose agar DAIGO for JP general test 300g
161-14695 0.0025N Poly(diallyldimethylammonium Chloride) Solution 500ml
399-01101 Brilliant Green Agar DAIGO, for JP General Test 300g
392-01831 Sabouraud-dextrose agar DAIGO for JP general test 300g
167-21341 フタル酸水素カリウム標準液 (Cとして : 1mg/ml 水溶液) 50ml
390-01131 Triple Sugar-Iron Agar DAIGO, for JP General Test 300g
146-08251 呋喃苯烯酸钠标准品Sodium Nifurstyrenate Standard 200mg
394-01771 Casein soya bean digest agar DAIGO for JP general test 300g
304-93526 Phos-tag (TM) Acrylamide AAL-107 5mM Aqueous Solution 0.3mL
395-01061 EMB寒天培地 , 日局試験用EMB Agar,”DAIGO”, for JP General Test 300g
394-00235 GPLP寒天培地?Daigo Glucose Peptone Agar With Lecithin & Polysorbate 80. 500g
345-01865 Ethylenediamine-N,N,N’,N’-tetraacetic Acid Disodium Salt Dihydrate (2NA) 500g
392-01875 Sabouraud-Dextrose Agar with Lecithin & Polysorbate 80 ”DAIGO” 500g
399-01885 Sabouraud-Dextrose Broth with Lecithin & Polysorbate 80 ”DAIGO’ 500g
025-03195 Buffer Solution Standard (Phosphate pH Standard Equimolal Solution) pH6.86 (25 degrees C) 500ml
028-03185 Buffer Solution Standard (Phthalate pH Standard Solution) pH4.01 (25 degrees C) 500ml
028-03205 Buffer Solution Standard (Tetraborate pH Standard Solution) pH9.18 (25 degrees C) 500ml
168-12145 Potassium Hydrogen Phthalate Standard Solution (as C:1mg/ml Water Solution) 500ml
392-01711 Buffered sodium chloride-peptone solution pH 7.0 DAIGO for JP general test 300g
393-01741 Rappaport Vassiliadis Salmonella Enrichment Broth DAIGO for JP general test 300g