Wako 和光纯药 011-27991 Anti Iba1, Goat Iba1抗体，山羊源多克隆抗体
名称：Anti Iba1, Goat
用途：for Immunochemistry
Storage Condition : Keep at -20 degrees C.
Application Data
Immunohistochemistry (fluorescent staining)
Immunohistochemistry of frozen section in mouse model of Alzheimer’s disease (APPNL-G-F mouse) brain cerebral neocortex using anti Iba1, goat polyclonal antibody at dilution of 1:1,000, and anti goat IgG, Alexa Flour 488-conjugated. Aβ were stained by 0.001% FSB solution(Amyloid fluorescent probe). The data were provided by Dr. Sakakibara, National Center for Geriatrics and Gerontology in Japan.
Immunohistochemistry of frozen section in rat(left) and mouse(right) brain cerebral cortex using anti Iba1, goat polyclonal antibody at dilution of 1:250, and anti goat IgG, Alexa Flour 488-conjugated.
The data were provided by Dr. Nakajima from Soka University in Japan.
Immunohistochemistry(DAB staining)
Sample: paraffin section of mouse brain frontal lobe
1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, biotin-conjugated
Antigen retrieval: 10mM citric acid buffer(pH6.0), 90℃, 10min
Western Blotting
The data were provided by Dr. Nakajima from Soka University in Japan.
Sample:
Microglia from rat primary culture 10μg
Neuron from rat primary culture 10μg
Astrocyte from rat primary culture 10μg
Cerebral cortex from rat brain 100μg
1st antibody: Anti Iba1, goat（1:1.000）
2nd antibody: Anti goat IgG, HRP-conjugated
Antibody Profile
| Antigen | Synthetic peptide corresponding to C-terminal of Iba1 |
|---|---|
| Buffer | TBS |
| Species cross-reactivity | Rat and Mouse |
| Antibody concentration | 0.5-0.7 mg/mL |
| Application | Immunohistochemistry(frozen section) 1:250-1,000 Immunohistochemistry(paraffin section) 1:250-1,000 Western blotting 1:1,000 |
Overview / Applications
| Outline | Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage and microglia.This product is goat polyclonal antibody that specifically recognize Iba1, and is available for a microglial marker.
Antigen: synthetic peptide(C-terminal of Iba1) |
|---|
Property
| Appearance | Liquid |
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Alias
- Anti Iba1, Goat Polyclonal Antibody
Anti AIF1, Goat Polyclonal Antibody
Anti IRT1, Goat Polyclonal Antibody