Wako和光纯药 小胶质细胞标记物 Iba1抗体 Microglia Marker – Iba1 Antibody Series

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Wako和光纯药 小胶质细胞标记物 Iba1抗体 Microglia Marker – Iba1 Antibody Series

A microglial markerMicroglia Marker – Iba1 Antibody Series

小胶质细胞标记物  小胶质细胞标记物 – Iba1 抗体系列

Microglia Marker - Iba1 Antibody Series

Iba1 is a calcium-binding protein with a molecular weight of 17,000 specifically expressed in macrophage/microglia. Recently, microglia has attracted attentions because neurological damage effect by production of NO, TNF-α, and IL-1β have been proved in addition to its role in neurotrophic effects/neuroprotective actions. These products are antibodies that specifically recognize Iba1, and are available for a microglial marker.
In particular, “Anti Iba1, Rabbit (for immunocytochemistry) Code No. 019-19741” is used by many worldwide researchers as a standard for microglial marker antibody. There are also many references in major journals such as Nature, Cell etc.

Iba1 是一种钙结合蛋白，分子量为 17,000，在巨噬细胞/小胶质细胞中特异性表达。最近，小胶质细胞除了在神经营养作用/神经保护作用中的作用外，还证明了通过产生 NO、TNF-α 和 IL-1β 产生的神经损伤作用而受到关注。这些产品是专门识别 Iba1 的抗体，可用于小胶质细胞标记。
特别是，“Anti Iba1, Rabbit (for Immunocytochemistry ) Code No. 019-19741 ”被全球许多研究人员用作小胶质细胞标记抗体的标准。在Nature、Cell等主要期刊上也有很多参考文献。

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Anti Iba1, Goat ➡ Click here for more information

SPICA Dye™ 568 Label Anti Iba1, Rabbit ➡ Click here for more information

  • Brochure
  • Reference List
  • Antibody Information
  • Select Flowchart (immunostain)
  • References
  • Application 1
  • Application 2
  • Application 3
  • Product List
  • Related Information

Antibody Information

Polyclonal Antibody Monoclonal Antibody
Product Name Most published
Anti Iba1, Rabbit
(for Immunocytochemistry)		 Anti Iba1, Rabbit
(for Paraffin Section)		 New
Anti, Iba1, Goat		 New
Anti Iba1,Rabbit,
SPICA Dye TM 568
-conjugate		 Anti Iba1, Rabbit,
Biotin
– conjugated		 Anti Iba1, Rabbit
Red Fluorochrome(635)
-conjugated		 Anti Iba1, Rabbit
(for Western Blotting)		 Anti Iba1,
Monoclonal Antibody
(NCNP24)		 Anti Human Iba1,
Monoclonal Antibody
(NCNP27)
Cat. Code No.
(Pkg. Size)		 019-19741
(50 µg)		 013-27691
(50 µg)		 011-27991
(100 µL)		 015-28011
(100μL)		 016-26461
(100 µL)		 013-26471
(100 µL)		 016-20001
(50 µg)		 012-26723
(10 µL)
016-26721
(50 µL)		 017-27591
(10 µL)
013-27593
(50 µL)
Antigen Synthetic peptide
(C-terminal of Iba1)		 Synthetic peptide
(C-terminal of Iba1)
Clone No. –
(Polyclonal Antibody)		 NCNP24 NCNP27
Subclass –
(Polyclonal Antibody)		 Mouse　IgG1 Mouse IgG2b ·κ
Conjugate – – – SPICA DyeTM 568
(Ex=556nm, Em=591nm)		 Biotin Red Fluorochrome
(Ex=634 nm, Em=654 nm)		 – – –
Concentration 0.5-0.7 mg/mL 0.5-0.7 mg/mL 0.6-0.7 mg/mL 0.5-0.6 mg/mL 0.5-0.7 mg/mL 0.9-1.6 mg/mL 0.9-1.3 mg/mL
Buffer TBS TBS TBS PBS, 0.05% NaN3 TBS 50% Glycerol / TBS, 0.05% NaN3
Cross-reactivity Mouse, rat, human,etc※ Mouse, rat Mouse, rat Mouse, rat Mouse, rat, human Mouse, rat, maroset Human
Applications ■Immunohistochemistry
(frozen section)
1:500-1,000

■Immunocytochemistry
1:500-1,000

 ■Immunohistochemistry
(paraffin section)
1:500-1,000		 ■Immunohistochemistry
(frozen section)
1:200-2,000

■Immunohistochemistry
(paraffin section)
1:200-2,000

■Western blotting
1:1,000

 ■Immunohistochemistry
(frozen section)
1:200-2,000		 ■Western Blotting
1:500-1,000		 ■Immunohistochemistry
(frozen section, DAB)
1:500-2,000

■Immunohistochemistry
(frozen section, fluorescent)
1:500-2,000

 ■Immunohistochemistry
(paraffin section, DAB)
1:100-1,000
References 1)2)3)4)5) 6)7)8) 9) – 10)11)12) 13)14)15) 16)17) 18)19) –
  • The paper using samples of dog1), cat2), pig3), marmoset4), and zebrafish5)
References  参考文献
  1. Ahn, J.H., et al.: Lab. Anim. Res., 28, 3, 165 (2012).
  2. Ide, T., et al.: J. Vet. Med .Sci., 72, 1, 99 (2010).
  3. Gaige, S., et al.: Neurotoxicology., 34, 135(2013).
  4. Rodriguez-Callejas, J.D. et al.: Front. Aging Neurosci., 8, 315(2016).
  5. Fantin, A., et al.: Blood, 116, 5, 829 (2010).
  6. Harlan, BA. et al.: Exp. Neurol, 327, 113219(2020).
  7. Boucher, AA. et al.: J. Thromb. Haemost, 18, 91(2020).
  8. Harland, M., et al.: J. Neurosci, 40, 1756(2020).
  9. Ryan, KM., et al.: J. Neuroimmunol., 338, 577082(2020).
  10. Jones, M. E., et al.: Brain Behav. Immun., 67, 355(2018).
  11. Rui, Y., et al.: Nutrients, 10, 1084(2018).
  12. Noyori, O., et al.: Clin. Transl. Immunology, 8, :e1074(2019).
  13. Inyang, K. E., et al.: Pharmacol. Res., 139, 1(2019).
  14. Yamamoto, M. et al.: Cell Rep., 28, 11, 2923(2019).
  15. Nozaki, K., et al.: Neuropharmacology, 162, 107835(2020).
  16. Sun, J.S. et al.: Mol. Med. Rep., 12, 2, 2677(2015).
  17. Grishchuk, Y., et al.: Am. J. Pathol., 186, 1, 199(2016).
  18. Wan, S., et al.: J. Neuroinflammation, 15, 31(2018).
  19. Chen, Y., et al.: Annals of Clinical and Translational Neurology,10.1002/acn3.513 (2017).

Select Flowchart (immunostain)

Microglia Marker - Iba1 Antibody Series

References

The number of publications using FUJIFILM Wako’s Iba1 antibody per year since 2010

Searched “Iba1 019-19741 Wako” by google scholar

Microglia Marker - Iba1 Antibody Series

Reference of major journal

Journal Title Number of references IF in 2018/2019
Nature 24 42.778
Cell 26 38.637
Science 1 41.845
Nature Medicine 9 36.130
Nature Neuroscience 42 20.071
Nature Immunology 4 20.479
Nature Biotechnology 3 36.558
Nature Methods 2 30.822
Neuron 22 14.415
Total 133

Application 1

Application data for immunohistochemistry, rat frozen section

Microglia Marker - Iba1 Antibody Series
Experimental condition

Code No. 019-19741/012-26723/016-26721
Tissue: Rat brain cortex
Section: Frozen section
Antibody concentration: 1/1,000

Code No. 016-26461/013-26471
Tissue: Rat brain cortex
Section: Frozen section
Antibody concentration: 1/200

These data were provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Application 2

Application Data –immunohistochemistry paraffin section-

016-27691:Anti Iba1, Rabbit(for Paraffin Section)

Microglia Marker - Iba1 Antibody Series
Experimental condition

Tissue: mouse and rat hippocampus neighborhood
Section: paraffin section
Antibody concentration: 1/1,000

Application 3

Application Data -western blotting-

016-20001: Anti Iba1, Rabbit (for Western Blotting)

Microglia Marker - Iba1 Antibody Series

Lane

  1. Iba1 protein (10 ng)
  2. Rat microglia (10 µg)
  3. Rat neuron (10 µg)
  4. Rat cortex (150 µg)
Experimental condition

SDS-PAGE: 5.5 % stacking gel, 12.5 % running gel, 100V
Blocking: 3 % skim milk in TBS at RT for 1h
Primary antibodies:anti-Iba1 antibody(1/1000) in 3 % skim milk in TTBS at 4 °C O/N
Secondary antibody: peroxidase labeled anti-rabbit antibody (1/5000) in 3 % skim milk in TTBS at RT for 1h

The data was provided by Sanagi,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Reference of major journal

Product List

Anti Iba1, Rabbit (for Immunocytochemistry)

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
Distributor
019-19741
50ug
List Price
JPY 40,000

In stock in Japan
Anti Iba1, Rabbit(for Paraffin Section)

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
013-27691
50ug
List Price
JPY 45,000

In stock in Japan
Anti Iba1, Rabbit, Biotin-conjugated

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Inventory
016-26461
100uL
List Price
JPY 45,000

In stock in Japan
Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
013-26471
100uL
List Price
JPY 48,500

In stock in Japan
Anti Iba1, Rabbit (for Western Blotting)

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
016-20001
50ug
List Price
JPY 40,000

In stock in Japan
Anti Iba1, Monoclonal Antibody(NCNP24)

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
012-26723
10uL
List Price
JPY 15,000

In stock in Japan
016-26721
50uL
List Price
JPY 45,000

In stock in Japan
Anti Human Iba1, Monoclonal Antibody(NCNP27)

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.
Comparison
Product Number
Package Size
Price
Inventory
017-27591
10uL
List Price
JPY 18,000

In stock in Japan
013-27593
50uL
List Price
JPY 40,000

In stock in Japan
Anti Iba1, Goat

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at -20 degrees C.

Comparison
Product Number
Package Size
Price
Inventory
011-27991
100uL
List Price
JPY 45,000

In stock in Japan
Anti Iba1, Rabbit, SPICA DyeTM 568-conjugated

for Immunochemistry

Manufacturer : FUJIFILM Wako Pure Chemical Corporation
Storage Condition : Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Inventory
011-28013
10uL
List Price
JPY 10,000

Inquire
015-28011
100uL
List Price
JPY 50,000

In stock in Japan

Related Information

Category

  1. Cell Culture
  2. Nerve Cell Culture
  3. Antibody
  1. Life Science
  2. Neuroscience
  3. Antibody

Content

6th review: Control of stroke via microglia-astrocyte crosstalkWako Blog
The roles of glial cells in brain function have been successively clarified. Given rapid, substantial, and various changes in glial cells in various brain diseases, it is assumed that these changes in…
5th review: Reverse translational research in psychiatry using human blood to predict brain microglial activityWako Blog
While the involvement of microglia, immune cells that play an important role in brain inflammation, has recently been suggested not only in neurodegenerative diseases but also in various other neurops…
4th review: Macrophages control the inflammation and subsequent neural repair after ischemic strokeWako Blog
Cerebrovascular disease (stroke) is a major cause of chronic functional deficits, such as being bedridden. Ischemic stroke comprises approximately 80% of cerebrovascular disease in Japan. In ischemic …
3rd review: Phagocytic cells in the brainWako Blog
Phagocytosis is an important process in the immune system. Exogenous viruses and pathogens as well as endogenous cell debris and abnormally aggregated proteins are removed by phagocytes, “professional…
2nd review: Origin of microglia and brain diseasesWako Blog
Microglia are tissue macrophages in the central nervous system (CNS) (i.e., brain and spinal cord) and play a frontline role in CNS immunity. With its unique developmental mode and biology being revea…
1st review: Neuropathic painWako Blog
In response to harmful stimuli, pain occurs in the body through the nociceptive system to trigger defensive behavior. However, chronic pain called neuropathic pain occurs when the nervous system is da…

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