| Home > Trace elements > Mg / Magnesium assay |
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Rev.150708 |
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| Easy to use. For cell lysates, serum and wide variety of biological samples. |
Pre-preatment free!
Species independent!
No toxic substance!
Applicable to microplate reader! |
[ Product Description ]
Magnesium is one of the most abundant minerals in the living cells, and plays important roles in many metabolic reactions such as polyphosphoric acid formation and photosynthesis in the chlorophyll.
[ Assay principle ]
Under alkaline condition, Xyridyl Blue-I(XB-I) – magnesium complex shows absorbance at 520 nm, and on the other hand, the absorbance by free XB-I at 600-680nm decreases.
[ Performance ]
1. Sensitivity:
2. Accuracy:
3. Intra assay variation:
4. Assay range: |
O.D. at 2 mg/dL standard will be 0.05 – 0.1(660nm vs 700nm)
±15% of known concentration
C.V. is less than 5%
0.05 – 3.5 mg/dL |
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| [ Specifications ] |
| Assay principle: |
XB-I method (Colorimetric assay at 520-660 vs 700 nm)
Measuring wavelength: 660nm (reference 700 nm) is recommended.
Measuring at 520 nm vs 700 nm shows higher sensitivity but may be susceptible to various factors. |
| Specifity: |
Magnesium (Mg) |
| Measuring range: |
0.05 – 3.5 mg/dL |
| Assay conditions: |
5 minutes at room temperature (25 – 37 °C) |
| Applications: |
Serum, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. This assay will be interfered by EDTA, hemoglobin, anticoagulants and high concentration of potassium. |
| Storage temperature: |
2 – 8°C |
| Expiry date: |
1 year after the day of manufacturing. |
| Required but not provided: |
Distilled water
96 well microtiter plate
Micropipettors and pipette tips
Microtiter plate reader (measuring wavelength 660/700 nm) |
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| [ Contents of this kit ] |
| R-1 Chelate color (ready to use): |
25 mL |
| Magnesium standard 2 mg/dL (ready to use): |
0.5 mL |
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| [ References: Assay examples ] |
| 1) |
Akanuma G, Kobayashi A, Suzuki S, Kawamura F, Shiwa Y, Watanabe S, Yoshikawa H, Hanai R, Ishizuka M:
Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.
J Bacteriol 196(22),p3820-3830(2014) doi: 10.1128/JB.01896-14 |
| 2) |
Sakamoto A, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A, Michael AJ, Igarashi K, Kashiwagi K:
Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor.
Int J Biochem Cell Biol 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010 |
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| Product name |
Catalog# |
Assay range |
Size |
Price |
| MC Reagent Magnesium Assay Kit (Xyridil Blue-I) |
CMG-035 |
0.05 – 3.5 mg/dL |
100 tests |
US$310 / €238 |
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Made in Japan.
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If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA).
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| Instruction manual |
Technical information |
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| [NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would be caused by such faults, we will be exempt from responsibility. |
日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。
