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Rev.151226 |
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| Suitable for immunohistocheistry, western blotting and ELISA. For research use only. |
| A novel biomarker for protein oxidation/nitration: |
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Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO–) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2–). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions.
To date, 6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO–-modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress. |
| An anti-6-nitrotryptophan monoclonal antibody has been developed by Prof. Yamakura at Juntendo University, which is suitable for immunohistochemistry, western blotting and ELISA. This antibody may provide a powerful tool for the investigation of nitrosative stress inside living bodies. |
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| Specifications |
| Antigen: |
6-nitrotryptophan |
| Form: |
Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG. |
| Subclass: |
Mouse IgG1(kappa), clone 117C. Protein A purified. |
| Specificity: |
Specific for 6-nitrotryptophan |
| Application: |
Immunohistochemistry, western blotting and ELISA |
| Storage: |
Less than -20°C |
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| Reference |
| 1) |
Ikeda K, Yukihiro Hiraoka B, Iwai H, Matsumoto T, Mineki R, Taka H, Takamori K, Ogawa H, Yamakura F:
Detection of 6-nitrotryptophan in proteins by Western blot analysis and its application for peroxynitrite-treated PC12 cells.
Nitric Oxide 16(1):18-28(2007) |
| 2) |
Degendorfer G, Chuang CY, Hammer A, Malle E, Davies MJ.
Peroxynitrous acid induces structural and functional modifications to basement membranes and its key component, laminin.
Free Radic Biol Med 89,p721-733(2015). doi: 10.1016/j.freeradbiomed.2015.09.018. |
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| Product name |
Code |
Content |
| Anti nitrotryptophan (NW) monoclonal antibody |
MNW-020P |
20 µg IgG |
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If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA).
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| Instruction manual (PDF) |
Technical information |
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| Related products:    |
Dityrosine (DT) ELISA |
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| [NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would be caused by such faults, we will be exempt from responsibility. |
日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。
