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Rev.120209 |
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| Suitable for immunohistocheistry, western blotting and ELISA. For research use only. |
| A novel biomarker for protein oxidation: |
DT staining of mouse skin (Dr. Sugiyama, Tottori Univ.) |
Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation. |
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| Detection of DT in protein samples: |
| Anti dityrosine monoclonal antibody clone 1C3 has been established by Dr. Toshihiko Osawa (Nagoya Univ.) and Dr. Yoji Kato (Univ. Hyogo)1). They have prepared this antibody using a dimer of 3-(p-hydroxyphenyl) propionic acid as a hapten, and screened using dityrosine-conjugated BSA. This antibody is highly specific for dityrosine, and can react with both free dityrosine and dityrosine containing protein or peptides. Suitable for immunohistochemistry, western blotting and ELISA. |
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| Specifications |
| Antigen: |
3-(p-hydroxyphenyl) propionic acid dimer-conjugated keyhole limpet hemocyanin. |
| Form: |
Lyophilized power. Reconstitute by 200 µL of distilled water. 100 µg/mL of IgG. |
| Subclass: |
Mouse IgG2a(kappa), clone 1C3. Protein A purified. |
| Specificity: |
Specific for free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. Cross reactivity have been tested for following analogues: Nitrotyrosine, chlorotyrosine, aminotyrosine, phosphotyrosine, tyrosine, 3-(p-hydroxyphenyl) propionic acid, phenylalanine, 3,4-dihydroxyphenylacetic acid (DOPA) |
| Application: |
Immunohistochemistry, western blotting and ELISA |
| Storage: |
Less than -20°C |
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| References |
| 1) |
Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T.
Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody.
Biochem Biophys Res Commun. 275(1), p11-15 (2000). |
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| Product name |
Code |
Content |
| Anti dityrosine (DT) monoclonal antibody |
MDT-020P |
20 µg IgG |
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If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA).
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| Instruction manual (PDF) |
Technical information |
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| Related products:    |
Dityrosine (DT) ELISA |
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| [NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would be caused by such faults, we will be exempt from responsibility. |
日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。
