Wako 018-22381 Anti DYKDDDDK tag, Monoclonal Antibody说明书

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Wako 018-22381 Anti DYKDDDDK tag, Monoclonal Antibody说明书

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for Immunochemistry
Storage Condition : Keep at -20 degrees C.
Host : Mouse
Clone No. : 1E6
Classification : Monoclonal

Overview / Applications

Outline The DYKDDDDK was consisted of 9 amino acids and has been used as the tag for detection and/or purification of recombinant protein.
Monoclonal anti DYKDDDDK tag antibody was established by fusing mouse myeloma cells and spleen cells of mice immunized with synthetic DYKDDDDK peptide which was coupled with keyhole limpet hemocyanin (KLH). This antibody was purified from mouse ascites.
This product is for research use only. Do not administer it to human.[Concentration]
Displayed on label.

[Formulation]
1 X PBS aqueous solution with 50% glycerol.

[Clone No.]
1E6

[Subclass]
IgG2b

[Specificity]
Specific for the DYKDDDDK peptide.

[Working Dilution]
Western blot 1:1,000 – 1:10,000
Immunoprecipitation 5 µg/20 µL 10% Protein A or Protein G beads slurry
Features Applicable to Western Blot analysis and Immunoprecipitation
Instructions Western Blot Analysis (1 : 1,000 – 1 : 10,000)
Immunoprecipitation (5 μg/20 μL 10% Protein A or Protein G beads slurry)

Property

Appearance Liquid
Composition 1xPBS aqueous solution with 50% glycerol.
Concentration 0.4 – 0.6 mg/mL Protein

Antibody Information

Antigen DYKDDDDK tag
Antigen Alias DYKDDDDK Peptide
DYKDDDDK
DYKDDDDK-tag
DYKDDDDK Epitope Tag
FLAG EPITOPE TAG
Ftag Antibody DYKDDDDK
FLAG
FLAG Epitope
FLAG peptide
Flag-tag