BalanCD HEK293 System

FUJIFILM Irvine Scientific的BalanCD HEK293可扩展系统提供了生长 和生产之间的 平衡,可 限度地提高哺乳动物细胞培养的生产力。

该多功能系统由化学成分明确、无动物源成分的BalanCD HEK293培养基和BalanCD HEK293 Feed组成,支持一系列应用,包括生产用于基因 的病毒载体、瞬时蛋白表达和重组蛋白生产。

·化学成分明确,不含动物源成分

·快速、可放大生产,用于生物生产的病毒载体和蛋白质

·支持用于各种HEK293细胞系

·提高瞬时蛋白质表达的生产力

·摇瓶、转瓶和生物反应器

BalanCD HEK293 Feed

Irvine欧文- HEK293 补料培养基(91166 – BalanCD HEK293 Feed),293补料,无血清、无动物源、化学成分限定。用于提高悬浮驯化293细胞的转染后蛋白产量。

货号:91166 BalanCD HEK293 Feed的配方可显着提高转染后驯化悬浮的 HEK293细胞系的蛋白质产量。

·增加活细胞密度和培养时间,以 化产率

·适用于各种HEK293细胞系

货号 产品名称 状态 规格 储存条件 保质期
91166 BalanCD® HEK293 Feed 液体 500mL 2–8℃, 避光 1年

Details
BalanCD HEK293 Feed is formulated to significantly increase protein yields in suspension-adapted HEK293 cell lines post-transfection.

Increase viable cell density and culture duration to maximize yield
Use the same Feed for a wide range of HEK293 cell lines
Fulfill regulatory requirements

Chemically-defined, animal component-free formula
Drug Master File (DMF) submitted to US FDA
Two-fold increase in titer compared to other commercially-available media

HEK293-6E cells were cultured in duplicate 125mL Erlenmeyer flasks with a 30 mL working volume. Cells were seeded at 3 x 105 cells/mL in BalanCD HEK 293 medium. PEI-mediated transfection was performed on day 3 to produce a biosimilar antibody. BalanCD HEK293 medium was supplemented with a 5% v/v addition of BalanCD HEK293 Feed on days 1-4 post-transfection.  Protein titer was measured on days 7, 9, and 11 post-transfection. Cultures terminated when viability dropped below 70%, (data not shown). Data shown represents the average of two duplicate cultures.

Specifications
Storage
Store at 2-8°C
Shelf Life
1 year from date of manufacture