WAKO LabAssay系列试剂盒(比色法)使用说明以及参考文献

WAKO LabAssay系列试剂盒(比色法)使用说明以及参考文献

Wako LabAssay系列试剂盒是利用实验动物的血清进行生化检验的试剂盒。使用微孔板检测,仅需少量样品,可同时进行多样品检测,检测常规的临床生化指标(脂质,酶,蛋白质,糖)。

类别 产品名称 产品编号 检测目标 规格 样品 检测范围 相关疾病
脂类 LabAssay™ Triglyceride 290-63701 甘油三酯 1000 test 血清 0~882mg/dL 心血管疾病
LabAssay™ Cholesterol 294-65801 胆固醇 1000 test 血清 0~593.2mg/dL 心血管疾病
LabAssay™ NEFA 294-63601 游离脂肪酸 750 test 血清 0~1.97mEq/L 糖尿病
LabAssay™ Phospholipid 296-63801 磷脂 1300 test 血清 0~596.1mg/dL 肝脏疾病
LabAssay ALP 291-58601 碱性磷酸酶 900 test 血清 >0.06mmol/L 肝脏和骨骼系统疾病
蛋白质 LabAssay™ Creatinine 290-65901 肌酸酐 500 test 血清/尿液 0~10mg/dL 肾脏功能障碍
LabAssay™ Glucose 298-65701 葡萄糖 1000 test 血清/血浆/尿液 0~500mg/dL 糖尿病
1.脂质
LabAssay™ Triglyceride (GPO·DAOS method)
  甘油三酯,是低密度脂蛋白和乳糜微粒的主要成分,在膳食脂肪的能量供应和运输等代谢过程中起到重要的作用。本品是检测N-(2-羟基-3-磺丙基)-3’5-二甲氧基苯胺钠盐进行酶促反应生成蓝色色素的吸光值,定量测定小鼠血清中的甘油三酯。使用微孔板检测,仅需少量样品,可同时进行多样品检测,也可以使用试管。

产品性能(吸光值):
ddH2O : Max.0.10
甘油三酯样品:0.09-0.25 300mg/Dl特异性(甘油三酯浓度):
对照血清浓度:within 12%之间
检测波长:600nm
LabAssay™ Cholesterol(Cholesterol Oxidase ・DAOS method)
  胆固醇是生物细胞膜的主要成分,是众多动物合成甾族化合物的前体物质。本品是利用N-乙基-N-(2-羟基-3-磺丙基)-3,5-二甲氧基苯胺钠(DAOS),通过酶浅蓝色显色反应,检测血清等样品中胆固醇量的试剂盒。与微孔板配套使用,可进行多样品检测。

产品性能(吸光值):
ddH2O : Max.0.11
胆固醇样品:0.13-0.65 200mg/Dl特异性(总胆固醇浓度):
可检测1000mg/dl的总胆固醇浓度检测波长:600nm
LabAssay™ NEFA
  游离脂肪酸(NEFA)是脂肪细胞中的中性脂肪被分解后从血中释放,在血清中与白蛋白结合,被末梢组织搬运,成为末梢组织重要的能源来源。检测游离脂肪酸的量,可用于调节由脂肪组织释放到肝脏的物质,有利于掌握类脂化合物代谢的动态。
本品可根据测量氧化缩合生成的青紫色色素的吸光值,检测样品中游离脂肪酸浓度。

产品性能(吸光值):
ddH2O : Max.0.07
游离脂肪酸样品:0.10-0.37 1mEq/L特异性(游离脂肪酸浓度):
对照血清浓度:within 15%之间检测波长:550nm
LabAssay™ Phospholipid
  磷脂是生物内细胞膜的主要构成成分,与脂肪的乳化、吸收和血液凝固等重要功能密切相关,本品由胆碱氧化酶和过氧化物酶等酶和显色底物组成,根据测量氧化缩合生成的浅蓝色色素的吸光值,检测样品中磷脂浓度。

产品性能(吸光值):
ddH2O : Max.0.14
磷脂样品:0.12-0.58 300mg/dL特异性(磷脂浓度):
对照血清浓度:within 10%之间检测波长:
主波长:600nm 副波长:700nm
2.酶
LabAssay™ ALP (Alkaline Phosphatase activity assay with p-Nitrophenylphosphate as a substrate)
  碱性磷酸酶的活性检测试剂盒。常用于检测培养的成骨细胞、实验动物血清和骨组织中的碱性磷酸酶活性。
碱性磷酸酶(ALP)广泛分布于肝脏、骨、小肠之中。特别在骨代谢研究领域方面被用作为骨形成指标之一。本品是用p–硝基苯磷酸作为底物的碱性磷酸酶检测试剂盒,根据测量p–硝基苯磷酸分解产物p–硝基酚的吸光值,检测样品中碱性磷酸酶活性。

产品性能:
检测范围:> 0.06mmol/L标准曲线范围:0-0.5mmol/L再现率:C.V.<10%检测波长:405nm
3.蛋白质
LabAssay™ Creatinine (Jaffe method)
  肌氨酸酐是由存在于肌肉、神经内的磷酸肌酸直接产生,或由肌酸脱水产生,经肾毛细血管球过滤后被排除体外的代谢产物。本品是利用Jaffe法,在碱性条件下检测苦味酸与肌氨酸酐反应生成的橙红色色素的吸光值,检测样品中肌氨酸酐含量。

产品性能(吸光值):
ddH2O : 0.010-0.020/10mg/dL
磷脂样品:0.400-0.500特异性(肌氨酸酐浓度):
对照血清浓度:within 10%之间检测波长:520nm
4.糖
LabAssay™ Glucose (Mutarotase-GOD method)
  葡萄糖是自然界分布最广且最为重要的一种单糖,葡萄糖在生物学领域具有重要地位,是活细胞的能量来源和新陈代谢中间产物。植物可通过光合作用产生葡萄糖。葡萄糖是细胞中重要的能量来源,能产生普遍的能量分子ATP。血糖水平是诊断多种代谢紊乱的关键参数之一。本品是测量葡萄糖在葡萄糖氧化酶作用下产生的过氧化氢与苯酚、4-氨基安替吡啉氧化缩合生成的红色色素吸光值,检测样品中葡萄糖的含量。

产品性能(吸光值):
ddH2O : Max. 0.03 200mg/dL
葡萄糖:0.40-0.55特异性(葡萄糖浓度):
对照血清浓度:within 12%之间检测波长:
主波长:505nm 副波长:600nm
※ 本页所载产品只供试验、研究用途。

Biochemical Assay Kits – LabAssay™

The LabAssay™ Series is a collection of biochemical assay kits for analyzing samples from humans, mice, and other animals. Since analyses are performed in microtiter plates, many samples can be concurrently evaluated, and only small sample amounts are needed.

※For Research Use Only, Not for Diagnostic Use

LabAssay™ Overview

Code No. Test item Target animals Sample Standard curve range Amount of sample Measurement time Pkg. Size
295-78901 Ammonia mouse / rat / human Blood 100~400 μg/dL 70 μL approx. 70 min. 700 tests
291-58601 ALP mouse / human Serum 0.0625~0.5 mmol/L 20 μL approx. 20 min. 900 tests
294-65801 Cholesterol mouse / human Serum 50~592.2 mg/dL 2 μL approx. 10 min. 1,000 tests
290-65901 Creatinine mouse / human Serum 2.5~10 mg/dL 50 μL approx. 40 min. 500 tests
298-65701 Glucose mouse / human Serum 50~500 mg/dL 2 μL approx. 10 min. 1,000 tests
294-63601 Non‐esterified fatty acid
(NEFA)
mouse / human Serum 0.5~1.97 mEq/L
※1 mEq of oleic acid=1 mmol
4 μL approx. 20 min. 750 tests
296-63801 Phospholipids mouse / human Serum 150~596.1 mg/dL 2 μL approx. 10 min. 1,300 tests
290-63701 Triglycerides mouse / human Serum 100~888 mg/dL 2 μL approx. 10 min. 1,000 tests

LabAssay™ Ammonia

Ammonia is mainly produced in the intestine or the kidney. Ammonia produced in the body is converted to urea through a series of reactions known as the urea cycle in the liver and eliminated through urine.

Assay Principle (Modified Fujii-Okuda Method)

Ammonia is converted to Dioxydiphenylamine by the reaction of Phenol and Sodium Pentacyanonitrosyl ferrate(III). A reaction of the Dioxydiphenylamine with Sodium Hypochlorite produces Indophenol ,which pigments blue, under alkaline conditions. LabAssay™ Ammonia is a kit used for the quantitative determination of ammonia nitrogen in samples by measuring absorbance of the blue color.

Standard Curve

00860_img01.png

Kit Contents

・Deproteinizing
・Chromogen Reagent A
・Chromogen Reagent B
・Chromogen Reagent C
・Ammonia Standard Solution
・Dilute olution for Standard
100 mL x 1 vial
50 mL x 1 vial
25 mL x 1 vial
50 mL x 1 vial
15 mL x 1 vial
20 mL x 1 vial

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:100~400 μg/dL (μg/100 mL)
:approx. 70 min.
:70 μL
:630 nm

Reference

  1. Inokuma, K. et al. Microb. Cell Fact.17, 153 (2018).

LabAssay™ ALP

Alkaline Phosphatase (ALP) is an enzyme distributed in a variety of tissues such as the liver, bone and small intestines in animals. Especially, it is used one of the Osteogenesis Markers in bone metabolism research area.

Assay Principle (Alkaline Phosphatase activity assay with p-Nitrophenyl Phosphate as a substrate)

p-Nitrophenylphosphate is hydrolyzed into p-Nitrophenol in the presence of Alkaline Phosphatase(ALP). LabAssay™ ALP is a kit designed to determine Alkaline Phosphatase activity in samples by measuring the amount of p-Nitrophenol released by p-Nitrophenylphosphate as a phosphatase substrate.

Standard Curve

00860_img02.png

Kit Contents

・Substrate Tablet
・Buffer Solution
・Quencher
・Standard Solution
20 tablets
100 mL x 1 vial
100 mL x 1 vial
10 mL x 1 vial

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:0.0625~0.5 mmol/L
:approx. 20 min.
:20 μL
:405 nm

Reference

  1. Ito, S. et al. : J. Pharmacol. Exp. Ther.333, 341 (2010). ※Extraction liquid of mouse kidney
  2. Matsuyama, A. et al. : Clin. Exp. Pharmacol. Physiol.45, 75 (2018). ※MC3T3-E1 cells, C2C12 cells
  3. Chiba, T. et al. : J. Atheroscler. Thromb.23, 1099 (2016). ※Mouse Plasma
  4. Kohno, Y. et al. : Stem Cell Res. Ther.8, 115 (2017). ※Periosteal mesenchymal stem cells
  5. Iwakura, T. et al. : J. Orthop. Res.27, 208 (2009). ※Extraction liquid of human culture cells
  6. Furuya, Y. et al. : J. Biol. Chem.286, 37023 (2011). ※Mouse serum
  7. Itoh, T. et al. : J. Biol. Chem.284, 19272 (2009). ※Extraction liquid of mouse culture cells

LabAssay™ Cholesterol

Cholesterol, a major component of cell membranes and the starting material in steroid synthesis in many animals, is a factor behind arteriosclerosis and other vascular diseases.

Assay Principle (Cholesterol Oxidase・DAOS method)

Hydrogen peroxide produced by a reaction of cholesterol and cholesterol oxidase let N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-Aminoantipyrin oxidize and condensate. LabAssay™ Cholesterol is a kit to determine total cholesterol by measuring absorbance of the blue color which is generated by the oxidative condensation reaction.

Standard Curve

00860_img03.png

Kit Contents

・Buffer
・Chromogen
・Standard Solution
150 mL x 2 vials
for 150 mL x 2 vials
10 mL x 1 vial

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:50~592.2 mg/dL (mg/100 mL)
:approx. 10 min.
:2 μL
:600 nm(Main), 700 nm(Sub)

Reference

  1. Kobayashi, Y. et al. : J. Pharmacogn. Nat. Prod., online (2015) doi: 10.4172/2472-0992.1000113  ※Extraction liquid of mouse Liver
  2. Gao, F. et al. : Evid. Based Complement. Alternat. Med., 2015, 801291 (2015). ※Extraction liquid of rat liver
  3. Yoshioka, H. and Onosaka, S. : Fundam. Toxicol. Sci.3, 151 (2016). ※Rat plasma
  4. Fujii, N. et al. : Aging Cell16, 508 (2017). ※Rat plasma
  5. Ushio, M. et al. : Am. J. Physiol. Endocrinol. Metab.305, E293 (2013).  ※Extraction liquid of Mouse tissue
  6. Kobayashi, Y. et al. : Biosci. Biotechnol. Biochem.74, 2385 (2010).  ※Extraction liquid of rat tissue

LabAssay™ Creatinine

Creatinine is a metabolite which is produced by creatine phosphate directly or dehydration of creatine in muscle and nerve. It is excreted from the body through kidney glomerular filtration.

Assay Principle (Jaffé method)

LabAssay™ Creatinine can be used to measure the creatinin levels in samples by Jaffe’s reaction where creatinine produces quantitatively an orange color with picric acid in alkaline condition.

Standard Curve

00860_img04.png

Kit Contents

・Depoteinizin Reagent
・Picric Acid Reagent
・0.75 mol/L Sodium Hydroxide Solution
・Standard Solution
150 mL x 1 vial
50 mL x 1 vial
50 mL x 1 vial
15 mL x 1 vial

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:2.5~10 mg/dL (mg/100 mL)
:approx. 40 min.
:50 μL
:520 nm

Reference

  1. Kawamoto, T. et al. : Glycative Stress Res.3, 15 (2016). ※Human urine
  2. Guan, Y. et al. : J. Pharmacol. Sci.135, 81 (2017). ※Mouse urine and plasma
  3. Ito, K. et al. : Biol. Pharm. Bull.38, 1169 (2015). ※Rat urine and plasma
  4. Tahara, Y. et al. : Med. Chem. commun.8, 415 (2017). ※Mouse Serum
  5. Nasrin, S. et al. : J. Pharmacol. Sci.122, 270 (2013). ※Rat urine
  6. Toyama, K. et al. : Br. J. Pharmacol.166, 1183 (2012).  ※Mouse plasma

LabAssay™ Glucose

Sugar is one of the most important sources of energy in biology. It is regulated by various factors within an organism. Glucose converges to a stable ratio of α-form and β-form in solutions.

Assay Principle (Mutarotase・GOD method)

α-D-Glucose is converted to β-D-Glucose by mutarotase. Hydrogen peroxide, which is produced by a reaction between β-D-Glucose and glucose oxidase(GOD), promotes oxidative condensation of phenol with 4-aminoantipyrine quantitatively. LabAssay™ Glucose is a kit used for the quantitative determination of glucose concentrations in samples by measuring absorbance of a red color which is generated by the oxidative condensation reaction.

Standard Curve

00860_img05.png

Kit Contents

・Buffer
・Chromogen Reagent
・Glucose Standard I
・Glucose Standard II
150 mL x 2 vials
for 150 mL x 2 vials
10 mL x 1 vial
10 mL x 1 vial

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:50~500 mg/dL (mg/100 mL)
:approx. 10 min.
:2 μL
:505 nm(Main), 600 nm(Sub)

Reference

  1. Yamashita, Y. et al. : Biosci. Biotechnol. Biochem.77, 888 (2013). ※Mouse plasma
  2. Narita, T. et al. : Exp. Gerontol.104, 127 (2018). ※Rat plasma
  3. Yamasaki, M. et al. : Food Sci. Technol. Res.21, 827 (2015). ※Mouse serum
  4. Fan, Y. et al. : J. Biomed. Sci.23, 56 (2016). ※Mouse serum
  5. Wang, W. et al. : J. Renin Angiotensin Aldosterone Syst.15, 384 (2014). ※Mouse serum
  6. Yamashita, Y. et al. : J. Nutr. Sci.1, e2 (2012). ※Mouse plasma
  7. Maesako, M. et al. : Neurobiol. Aging.33, 1011 (2012). ※Mouse serum

LabAssay™ NEFA

NEFA (Non‐esterified fatty acid) in the blood is transported complexed with an albumin to peripheral tissues. They are important sources of fuel for the peripheral tissues. The concentration of NEFA in the blood is regulated by a release from the adipose tissues, a consumption in the periphral tissues or a take up from the liver.

Assay Principle (ACS・ACOD method)

NEFA (Non‐esterified fatty acid) forms Acyl-CoA in the presence of Acyl-CoA synthetase (ACS). Hydrogen peroxide, which is produced by a reaction between the Acyl-CoA and Acyl-CoA oxidase (ACOD), promotes oxidative condensation of 3-methyl-N-ethyl-N-(β-hydroxyethyl)-aniline (MEHA) with 4-aminoantipyrine. LabAssay™ NEFA can be used for the quantitative determination of NEFA in samples by measuring absorbance of a blue purple color which is generated by the oxidative condensation reaction.

Standard Curve

00860_img06.png

Kit Contents

・Chromogen Reagent A
・Solvent A
・Chromogen Reagent A
・Solvent B
・NEFA Standard Solution (1 mEq/L of Oleic acid)
for 10 mL x 6 vials
65 mL x 1 vial
for 20 mL x 6 vials
130 mL x 1 vial
10 mL x 1 vial

 ※amount of oleic acid; 1mEq=1mmol

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:0.5~1.97 mEq/L
:approx. 20 min.
:4 μL
:550 nm

Reference

  1. Kobayashi, Y. et al. : J. Pharmacogn. Nat. Prod., online (2015) doi: 10.4172/2472-0992.1000113 ※Extraction liquid of mouse liver
  2. Gao, F. et al. : Evid. Based Complement. Alternat. Med., 2015, 801291 (2015). ※Extraction liquid of rat liver
  3. Ogawa, K. et al. : Reprod. Med. Biol., online (2018). doi.org/10.1002/rmb2.12084 ※Pig cumulus-oocyte complexes
  4. Wang, F. et al. : J. Mol. Endocrinol.52, 133 (2014). ※Mouse plasma
  5. Chang, Y. C. et al. : EMBO Mol. Med.5, 1165 (2013). ※Mouse plasma
  6. Uchida, K. et al. : Exp. Anim.58, 181 (2009). ※Mouse serum

LabAssay™ Phospholipid

Phospholipids are known as not only as major component of cell membranes but also perform vital functions within the body such as emulsification and absorption of fats or coagulation of blood.

Assay Principle (Choline Oxidase・DAOS method)

Phospholipids are hydrolyzed by Phospholipase D to release hydrogen peroxide. The formed hydrogen peroxide promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay™ Phospholipid is a kit to determine Phospholipid concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Standard Curve

00860_img07.png

Kit Contents

・Buffer
・Chromogen Substrate
・Standard Solution
50 mL x 8 vials
for 50 mL x 8 vials
10 mL x 2 vials

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:50~500 mg/dL (mg/100 mL)
:approx. 10 min.
:2 μL
:505 nm(Main), 600 nm(Sub)

Reference

  1. Xu, Q. et al. : Biosci. Biotechnol, Biochem.77, 1390 (2013). ※Extraction liquid of mouse liver
  2. Tatematsu, Y. et al. : Biol. Pharma. Bull.41, 319 (2018). ※Liposome
  3. Kuge, H. et al. : J. Biol. Chem.289, 26783 (2014). ※Liposome
  4. Kessler, E. C. et al. : J. Dairy. Sci.97, 5481 (2014). ※Bovine
  5. Cheng, L. et al. : Transplantation90, 127 (2010). ※Rat

LabAssay™ Tryglyceride

Triglycerides are neutral fats consisting of three fatty acids esterified to a glycerol backbone. There are triglycerides, cholesterol, phospholipids, free fatty acids and fat-soluble vitamins as lipid-soluble substances in the blood.

Assay Principle

Triglycerides are converted to glycerol-3-phosphate by lipoprotein lipase and glycerolkinase. Hydrogen peroxide, which is produced by a reaction between the glycerol-3-phosphate and glycerol-3-phosphate oxidase(GPO), promotes oxidative condensation of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt(DAOS) with 4-aminoantipyrine. LabAssay ™ Triglyceride can be used to detect triglycerides concentration in samples by measuring absorbance of a blue color which is generated by the oxidative condensation reaction.

Standard Curve

00860_img08.png

Kit Contents

・Buffer
・Chromogen Substrate
・Standard Solution
105 mL x 3 vials
for 105 mL x 3 vials
10 mL x 1 vial

Performance

・Standard curve range
・Measurement time
・Amount of sample
・Measurement wavelength
:100~888 mg/dL (mg/100 mL)
:approx. 10 min.
:2 μL
:600 nm(Main), 700 nm(Sub)

Reference

  1. Gao, F. et al. : Evid. Based Complement. Alternat. Med.2015, 801291 (2015). ※Extraction liquid of rat liver
  2. Funakoshi, T. et al. : Biochem. Biophys. Rep.13, 39 (2018). ※Rat muscle satellite cell
  3. Moser, V. A. and Pike, C. J. : eNeuro4, e0077-17 (2017). ※Rat plasma
  4. Fujii, N. et al. : Aging Cell16, 508 (2017). ※Rat plasma
  5. Wang, F. et al. : J. Mol. Endocrinol.52, 133 (2014). ※Rat plasma
  6. Kato, H. et al. : J. Hepatol.60, 1032 (2014). ※Extraction liquid of mouse liver
  7. Fujimori, K. et al. : Prostaglandins. Other. lipid. Mediat.94, 96 (2011). ※Extraction liquid of mouse culture cells

图片仅供参考,请以实物为准。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。