日本老化Jaica-MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)

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MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
Easy to use. For cell lysates, serum, plasma and wide variety of biological samples.
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
Pre-preatment free!
Species independent!
No toxic substance!
Applicable to microplate reader!
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)		 [ Product Description ]
Inside human body, approximately 95&#37 of copper in the plasma is attached to alpha-2- globulin, ceruloplasmin and some enzymes with ferroxidase activity. The main role of protein containing copper is oxidation-reduction function. Copper deficiency is known to be associated with some diseases such as heart disease, osteoporosis, osteoarthritis, Menkes syndrome and Wilson’s disease. It has been also reported that copper deficiency may cause reduction of anti-oxidative function in vivo. On the other hand, the excess copper is toxic. This kit is a simple colorimetric assay which can detect copper precisely in biological fluids in a short period of time (about 10 minutes).

[ Assay principle ]
In this assay system, copper is dissociated from ceruloplasmin and other copper containing proteins, by denaturing agent in acidic condition. Free and protein-associated copper will form a copper -3-5 DiBr-PAESA complex, which can be detected by absorbance at 580 nm.

[ Performance ]

1. Sensitivity:
2. Accuracy:
3. Intra assay variation:
4. Assay range:		 O.D. at 200 µg/dL standard will be 0.03 – 0.1
±10% of known concentration
C.V. is less than 5%
3 – 400 µg/dL
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
[ Specifications ]
Assay principle: DiBr-PAESA method (Colorimetric assay at 580 (570-590) nm)
Specifity: Specific to Cu2+ and Cu+
Measuring range: 3 – 400 µg/dL
Time to answer: 10 minutes
Applications: Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Will be interfered by EDTA.
Storage temperature: 2 – 8°C (don’t freeze).
Expiry date: 1 year after the day of manufacturing.
Required but not provided: Distilled water
96 well microtiter plate
Micropipettors and pipette tips
Microtiter plate reader (measuring wavelength 580 nm)
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
[ Contents of this kit ]
R-1 Buffer (ready to use): 24 mL
R-2 Chelate color (ready to use): 0.5 mL
Copper standard 200 µg/dL (ready to use): 1.2 mL
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
[ References: Assay examples ]
1) Kazuma Nakanishi, Masahiro Tomita and Katsuya Kato:
Synthesis of amino-functionalized mesoporous silica sheets and their application for metal ion capture.
J Asian Ceramic Societies 3(1), p70-76(2015) doi:10.1016/j.jascer.2014.10.011
2) Nobuhiko Asada, Aoi Kedamori, Yumiko Kusano and Tetsuro Takeuchi:
Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes.
J Life Sci 8(6),p517-521(2014)
3) Yasuyuki Sakurai, Itsuki Anzai, and Yoshiaki Furukawa:
A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase.
J Biol Chem 289(29),p20139-20149(2014). doi: 10.1074/jbc.M114.567677
4) Sakamoto, Terui Y, Yamamoto T, Kasahara T, Nakamura M, Tomitori H, Yamamoto K, Ishihama A, Michael AJ, Igarashi K, Kashiwagi K:
Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor.
Int J Biochem Cell Biol. 44(11),p1877-1886(2012). doi: 10.1016/j.biocel.2012.07.010
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
Product name Catalog# Assay range Size Price
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA) CCU-400 3-400 µg/dL 100 tests US$370 / €285

Made in Japan.
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)

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Instruction manual Technical information
MC Reagent Copper Assay Kit (3,5-DiBr-PAESA)
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.

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日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。