日本老化Jaica-MC Reagent Fe Assay Kit (Ferrozine)

Home > Trace elements > Fe/iron assay

Rev.140409

Easy to use. For cell lysates, serum, plasma and wide variety of biological samples.

[ Product Description ]

Pre-preatment free!
Species independent!
No toxic substance!
Applicable to microplate reader!

Iron is a mineral which binds many proteins and enzymes, and plays important roles in living bodies. MC-Reagent Fe Assay Kit provides a simple and convenient tool for the determination of Ferrous and/or Ferric ion in a variety of biological samples such as cell lysates, tissue homogenates, serum, plasma, saliva, urine, tissue extract, plant extract, hair and food extract.

[ Performance ]
1. Sensitivity: O.D. at 200 µg/dL standard will be 0.08 – 0.25
2. Accuracy: ±10% of known concentration
3. Intra assay variation: C.V. is less than 5%
4. Assay range: 5 – 1,000 µg/dL

Not be affected by following substances.
1. Hemoglobin: up to 70 mg/dL
2. Bilirubin: up to 90 mg/dL
This assay will be interfered by EDTA.

Good liearity for full assay range	Good correlation with ICP
Easy to apply to tissue samples

[ Specifications ]

Assay principle:	Ferrozine method (Colorimetric assay at 560nm)
Specifity:	Specific to Fe²⁺ and Fe³⁺
Measuring range:	5 – 1,000 µg/dL
Sample volume:	40 µL
Assay conditions:	10 minutes at room temperature (25 – 37 °C)
Applications:	Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Metal chelatos, for example EDTA may interfere with this assay.
Storage temperature:	2 – 8°C (don’t freeze).
Expiry date:	1 year after the day of manufacturing.
Required but not provided:	96-well plate Pipeting devices such as 8-channel (50-200 µL) micropipettor and tips 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. Microtiter plate reader (measuring wavelength between 540 and 580nm. 560nm is recommended.)

[ Content of this kit ]

R-A Buffer (ready to use):	1 x 40 mL
R-R Chelate color (ready to use):	1 x1.6 mL
Iron standard 200 µg/dL (ready to use):	1 x 8 mL

[ References: Assay examples ]

1)	Y Ikeda, S Tajima, YI-Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya and T Tamaki: Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes. PLoS ONE 7(7),e40465(2012) doi: 10.1371/journal.pone.0040465
2)	Tsugawa H, Suzuki H, Matsuzaki J, Hirata K, Hibi T: FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach. Free Radical Biology and Medicine 52(6),p1003-1010(2012) doi: 10.1016/j.freeradbiomed.2011.12.011.
3)	M Nakaya, M Tajima, H Kosako, T Nakaya, A Hashimoto, K Watari, H Nishihara, M Ohba, S Komiya, N Tani, M Nishida, H Taniguchi, Y Sato, M Matsumoto, M Tsuda, M Kuroda, K Inoue and H Kurose: GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance. Nature Communications 4, 1532 (2013) doi:10.1038/ncomms2540
4)	Y Ikeda, H Enomoto, S Tajima, YI Ishizawa, Y Kihira, K Ishizawa, S Tomita, K Tsuchiya, T Tamaki: Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice. American Journal of Physiology – RenalPhysiology 304:F1028-F1036(2013) DOI:10.1152/ajprenal.00473.2012
5)	Y Ikeda, I Ozono, S Tajima, M Imao, Y Horinouchi, YI Ishizawa, Y Kihira, L Miyamoto, K Ishizawa, K Tsuchiya, T Tamaki: Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction. PLoS ONE 9(2), e89355(2014) doi:10.1371/journal.pone.0089355

Product name	Catalog#	Assay range	Size	Price
MC Reagent Fe Assay Kit (Ferrozine)	CFE-005	5-1,000 µg/dL	100 tests	US$330 / €254

Made in Japan.

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日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。