日本老化Jaica-Anti HEL monoclonal antibody(clone 5F12)


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Anti HEL monoclonal antibody(clone 5F12) Anti HEL monoclonal antibody(clone 5F12)
Anti HEL monoclonal antibody(clone 5F12)
Suitable for immunohistocheistry and ELISA. For research use only.
A new biomarker for lipid peroxidation
Anti HEL monoclonal antibody(clone 5F12) Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.
Anti HEL monoclonal antibody(clone 5F12)
Cisplatin-treated rat kidney.
(Dr. Sugiyama, Tottori Univ.)
Anti HEL monoclonal antibody(clone 5F12)
Detection of HEL at Human atherosclerotic lesions.
(Dr. Naito)
Specifications
Clone #: 5F12
Subclass: IgG1 kappa
Antigen: N epsilon hexanoyl-Keyhole limpet hemocyanin (HEL-KLH)
Specificity: Specific to Hexanoyl-Lys adducts.
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950. 100 µg/mL of IgG.
Application: Immunohistochemistry
(Recommended antibody concentration is 2 µg/mL on paraformaldehyde fixed tissue), and ELISA
Storage: Less than -20°C
Anti HEL monoclonal antibody(clone 5F12)
References
1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.
4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).
5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.
Anti HEL monoclonal antibody(clone 5F12)
Product name Code Specifications Application NOTICE
Anti HEL monoclonal antibody(clone 5F12) MHL-021P 20 µg of IgG Immunohistochemistry Current product
Anti HEL monoclonal antibody(clone 5H4) MHL-020P 20 µg of IgG Immunohistochemistry Discontinued

Made in Japan.

IMPORTANT NOTICE
The clone of anti HEL monoclonal antibody has been changed from 5H4 (code. MHL-020P) to 5F12 (code. MHL-021P). If you need old product, please contact our technical support.

Related products:&nbsp&nbsp&nbsp Hexanoyl-Lys ELISA kit  &nbsp
Anti HEL monoclonal antibody(clone 5F12)

If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA)
.

Instruction manual (PDF) Immunohistochemical procedure Technical information
Comparison of clone 5F12 and 5H4 (PDF)
Anti HEL monoclonal antibody(clone 5F12)
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日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒,并且处于该领域的领先地位。JaICA氧化应激相关产品:DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析,以及微量元素分析。详情请咨询JaICA中国区代理商:上海金畔生物科技有限公司。