| Home > Products > Antibodies > Anti 4-HNE antibody |
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Rev.090316 |
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| Suitable for immunohistocheistry and western blotting. For research use only. |
| About 4-hydroxy-2-nonenal (4-HNE) |
Structure of 4-hydroxy-2-nonenal |
Reactive oxygen species (ROS) are involved in lipid peroxidation inside the living bodies, and membrane lipids are one of the major targets of ROS. During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. |
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High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999) |
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| Specifications |
| Clone #: |
HNEJ-2 |
| Immunogen: |
4-HNE-modified Keyhole Limpet Hemocyanin |
| Subclass: |
Mouse IgG1(kappa)
Prepared as ascite, and ammnonium sulphate purified. |
| Form: |
Lyophilized Powder (containing 20 µg or 100 µg of IgG) |
| Specificity: |
This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde. |
| Applications: |
Immunohistochemistry and western blotting. |
| Storage: |
Less than -20°C for 5 years. When transport, stable at least 7 days at room temperature. |
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| References |
| Immunohistochemistry: |
T.Tanaka, Y.Nishiyama, K.Okada, K.Hirota, M.Matsui, J.Yodoi, H.Hiai, and S.Toyokuni:
Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion. Lab. Invest. 77(2), p145-155 (1997) |
| Western blotting: |
S.Toyokuni, N.Miyake, H.Hiai, M.Hagiwara, S.Kawakishi, T.Osawa and K.Uchida:
The monclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct.
FEBS Lett. 359, p189-191 (1995) |
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| Product name |
Code |
Content |
| Anti 4-HNE monoclobal antibody |
MHN-020P |
20 µg of IgG/ vial |
| MHN-100P |
100 µg of IgG/ vial |
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Made in Japan
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If you are in the U.S., please click here
to Genox Corporation (Los Angeles, CA).
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| Instruction manual (PDF) |
Immunohistochemical procedure |
Technical information |
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| [NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
We are making efforts to prevent errors or mistakes on preparing web site documents, instruction manuals and products.
But even if some damage would causedby such faults, we will be exempt from responsibility. |
日本衰老控制研究所 Japan Institute For the Control of Aging (JaICA) 专注于衰老研究领域。提供DNA氧化损伤检测试剂盒，并且处于该领域的领先地位。JaICA氧化应激相关产品：DNA氧化损伤检测、脂质氧化检测、蛋白氧化检测、抗氧化分析，以及微量元素分析。详情请咨询JaICA中国区代理商：上海金畔生物科技有限公司。
