日本和光纯药Wako海藻酸钠系列产品 9005-38-3

日本和光纯药Wako海藻酸钠系列产品 9005-38-3

Wako 和光纯药 191-09965 Sodium Alginate 500-600 海藻酸钠500~600 Wako 1st Grade 500 g 9005-38-3
Wako 和光纯药 192-09995 Sodium Alginate 300-400 海藻酸钠300~400 Wako 1st Grade 500 g 9005-38-3
Wako 和光纯药 194-13321 Sodium Alginate 80-120 海藻酸钠80-120 Wako 1st Grade 100 g 9005-38-3
Wako 和光纯药 196-13325 Sodium Alginate 80-120 海藻酸钠80-120 Wako 1st Grade 500 g 9005-38-3
Wako 和光纯药 199-09961 Sodium Alginate 500-600 海藻酸钠500~600 Wako 1st Grade 100 g 9005-38-3
Wako 和光纯药 190-09991 Sodium Alginate 300-400 海藻酸钠300~400 Wako 1st Grade 100 g 9005-38-3

供应日本和光WAKO葡聚糖 Dextran CAS:9004-54-0

供应日本和光WAKO葡聚糖 Dextran CAS:9004-54-0

Wako 和光纯药 049-22331 Dextran 40,000 右旋糖苷/葡聚糖 40,000 Wako 1st Grade 50 g 9004-54-0
Wako 和光纯药 041-30525 Dextran 60,000 右旋糖苷/葡聚糖 60,000 No grade 500 g 9004-54-0
Wako 和光纯药 046-32591 Dextran 150,000 葡聚糖150,000 Wako 1st Grade 50 g 9004-54-0
Wako 和光纯药 047-30522 Dextran 60,000 右旋糖苷/葡聚糖 60,000 No grade 25 g 9004-54-0
Wako 和光纯药 041-22612 Dextran 200,000 右旋糖苷/葡聚糖 200,000 for Leukocite Seperation 25 g 9004-54-0
Wako 和光纯药 22500-100 Dextran [MW 200,000-300,000] 葡聚糖 200,000-300,000 100 g 9004-54-0
Wako 和光纯药 042-00542 Dextran 150,000   25 g 9004-54-0
Wako 和光纯药 042-32593 Dextran 150,000 葡聚糖150,000 Wako 1st Grade 250 g 9004-54-0
Wako 和光纯药 043-22611 Dextran 200,000 右旋糖苷/葡聚糖 200,000 for Leukocite Seperation 100 g 9004-54-0

Wako 四水合氯化亚铁 FeCl2·4H2O 13478-10-9

Wako 四水合氯化亚铁 FeCl2·4H2O 13478-10-9

英文名称:Iron(Ⅱ) Chloride Tetrahydrate, 99.9%
中文名称:四水合氯化亚铁,99.9%
品牌:Wako
CAS No.:13478-10-9
储存条件:室温
纯度:(PuA)

品牌 货号 产品名称 等级 规格 CAS No.
Wako
和光纯药
091-04412 Iron(Ⅱ) Chloride Tetrahydrate, 99.9%

四水合氯化亚铁,99.9%

No grade 25 g 13478-10-9

Wako 034-13632 Chitin 壳多糖 替代品:039-25772

Wako 034-13632 Chitin 壳多糖 替代品:039-25772

英文名称:Chitin
中文名称:壳多糖
品牌:Wako
CAS No.:1398-61-4
储存条件:室温

Wako 039-25772 Chitin 壳多糖 25 g 1398-61-4
Wako 033-25775 Chitin 壳多糖 500 g 1398-61-4
Wako 034-13632替代品:039-25772 Chitin 壳多糖 25 g 1398-61-4
Wako 031-14301 Chitosan, Water Soluble 脱乙酰壳多糖水溶液 1 g
Wako 037-14303 Chitosan, Water Soluble 脱乙酰壳多糖水溶液 10 g
Wako 038-13635替代品:033-25775 Chitin 壳多糖 500 g 1398-61-4
Wako 033-12943 Chitosanase, from Batillus punits BN-262 脱乙酰壳多糖酶 250 units
Wako 037-12941 Chitosanase, from Batillus punits BN-262 脱乙酰壳多糖酶 50 units
Wako 038-14311 Chitinase, from Bacillus sp. 壳多糖酶(芽胞杆菌属由来) 100 mg

Wako 残留DNA提取试剂盒 DNA with DNA Extractor® Kit

Wako 残留DNA提取试剂盒 DNA with DNA Extractor® Kit

This article was written by Hiroki Fukuchi, Life Science Research Laboratories of FUJIFILM Wako Pure Chemical Corporation, Japan.

导言
因为许多生物药物,包括疫苗,都是由培养的细胞或微生物(如大肠杆菌指出宿主细胞来源的DNA可能存在于合成的药物物质和产物中.鉴于残留DNA可能转移宿主细胞或病毒衍生的癌基因,病毒DNA可能导致感染事件,定量检测残留DNA是制造业的重要测试,也是生物制药过程验证等测试的一部分。正如一份报告所指出的那样,每一剂量的残余DNA最多可达100 pg,这对于生物制药来说是可以接受的,1)不仅在美国和欧洲国家,而且在其他国家,越来越需要对宿主细胞中的残留DNA进行定量检测。为了检测残留DNA的痕迹,必须从样品中提取出高收率的残留DNA。本文旨在介绍DNA提取器的用途。®用于检测残留DNA作为DNA纯化试剂的试剂盒。

DNA提取器®试剂盒(#295-50201,Fujifilm Wako纯化学公司)
在对生物制品中的总DNA进行检测和定量之前,有必要从蛋白质等其他生物成分中分离纯化生物制品中的DNA。一般来说,DNA的分离是通过蛋白酶介导的样品消化,然后用苯酚和/或氯仿提取的。然而,这种方法的缺点是,必须使用有害的苯酚和氯仿,需要时间和人力的提取,虽然可以获得相当高纯度的DNA。此外,二氧化硅载体固相萃取,由于载体吸附导致DNA丢失,对DNA的提取效果不理想。有机溶剂的提取也不利于DNA的有效回收,这是DNA提取试剂需要解决的关键问题之一。

DNA提取器®试剂盒(产品代码:295-50201),我们于1992年推出,解决了上述限制,使用钠碘提取高纯度的DNA,通过简单的程序。

DNA提取原理®试剂盒
工具包的组成部分*含有碘化钠和表面活性剂,当2-丙醇加入时,它们通过溶解蛋白质和其他生物成分,选择性地沉淀(共沉淀)核酸(主要是DNA)和糖原,作为蛋白质增溶剂(潮致离子)。2)与上述方法不同,纯化步骤被简化为在没有载体或有机溶剂的情况下产生沉淀物,从而能够以较高的收率提取微量DNA。

*工具包的组成部分:
碘化钠溶液,N-月桂酸盐溶液,洗涤液(A),
洗涤液(B),糖原溶液

DNA提取器提取DNA的实例®试剂盒和总DNA定量
本文介绍了一份关于在稀释剂或辅料存在的情况下DNA产量的报告,该报告最初发表在瓦科纯化学杂志第60卷第3期(1992年)第28页。本实验通过在磷酸盐缓冲液中溶解常用作稀释剂或辅料(如精氨酸、尿素)或蛋白质(BSA和人γ-球蛋白)的常用或过量的物质制成模型溶液,并在每个模型溶液中加入一定量的小牛胸腺脱氧核糖核酸(PG)。然后,根据dna提取器标签上的说明,从模型溶液的400μL中提取dna。®基特。所得沉淀物溶于500μL磷酸盐缓冲液中,用阈值法测定总dna。®总DNA分析系统,*3,4)来测量DNA的产量。测量结果见表1。

Introduction

Since many biopharmaceuticals, including vaccines, are manufactured from cultured cells or microorganisms such as Escherichia coli, it is pointed out that host cell-derived DNA may remain in resultant drug substances and products. Given the possibility that the residual DNA may transfer host cell- or virus-derived oncogenes and that viral DNA may cause infectious events, quantitative detection of residual DNA is important testing for manufacturing and as part of testing such as process validation for biopharmaceuticals. As indicated by a report recommending that up to 100 pg of residual DNA per dose is acceptable for biopharmaceuticals,1) there is increasing need for quantitative detection of residual DNA from host cells as a quality test not only in the US and European countries but also in other countries. To detect traces of residual DNA, it is necessary to extract traces of residual DNA from a sample at a high yield. This article is intended to introduce the usefulness of DNA Extractor® Kit in detecting traces of residual DNA as a DNA purification reagent.

DNA Extractor® Kit (#295-50201, FUJIFILM Wako Pure Chemical Corporation)

Prior to detection and quantification of total DNA in a biologics, it is necessary to separate and purify DNA in the biologics from other biological components such as proteins. In general, DNA is isolated through protease-mediated digestion of specimen followed by extraction with phenol and/or chloroform. However, this method has disadvantages that deleterious phenol and chloroform have to be used and that time- and labor-consuming extraction is required, although quite highly pure DNA can be obtained. In addition, solid-phase extraction via silica carriers, which results in loss of DNA due to adsorption on carriers, is not ideal to recover traces of DNA. Extraction with organic solvents is also not favorable with inefficient recovery of traces of DNA, which is a key issue to be addressed with DNA extraction reagents.

DNA Extractor® Kit (Product code: 295-50201), which we launched in 1992, resolves the aforementioned limitations by using sodium iodide to extract highly pure DNA at high yields through simple procedures.

Principle of DNA Extractor® Kit

The components of the kit* contain sodium iodide and surfactants, which act as protein solubilizers (chaotropic ions) by solubilizing proteins and other biological components in specimens to precipitate (coprecipitate) nucleic acids (mainly DNA) and glycogen selectively when 2-propanol is added.2) Purification steps are simplified to produce precipitates without carriers or organic solvents, unlike the aforementioned methods, enabling the extraction of traces of DNA at high yields.

* Components of the kit:
Sodium Iodide Solution, Sodium N-Lauryl Sarcosinate Solution, Washing Solution (A),
Washing Solution (B), Glycogen Solution

Example of DNA extraction with DNA Extractor® Kit and total DNA quantification

A report on yields of DNA in the presence of diluents or excipients with the use of the kit, which was originally published on page 28 in Wako Pure Chemical Jiho Vol. 60 No. 3 (1992), is introduced here. In this experiment, model solutions were prepared by dissolving usual or excessive doses of substances often used as diluents or excipients (e.g., arginine, urea) or proteins (BSA and human γ-globulin) in phosphate-buffered saline, and a given amount (pg) of calf thymus DNA was added to each model solution. Subsequently, DNA was extracted from 400 μL of model solution according to the instructions on the label of DNA Extractor® Kit. The obtained precipitates were dissolved in 500 μL of phosphate-buffered saline, and total DNA was quantified using Threshold® Total DNA assay system,*3,4) to measure the yield of DNA. Measurement results are presented in Table 1.

Table 1. Yields of DNA in the presence of diluents or excipients
Diluent and its amount Amount of DNA (pg) Yield of DNA (%)
Sorbitol 200 mg/mL
50 mg/mL
50
50
95
94
Maltose 400 mg/mL
200 mg/mL
50 mg/mL
50
50
50
89
102
98
Mannitol 200 mg/mL 50 84
Dextrose 200 mg/mL 50 81
Sucrose 200 mg/mL 50 92
Urea 1 mol/L 50 106
Arginine 200 mg/mL 50 110
γ-globulin 60 mg/mL
60 mg/mL
60 mg/mL
10
5
2.5
102
84
88
BSA 200 mg/mL 10 95

For all five sugars at one or more concentrations, 80% to 110% of DNA was recovered. For arginine and urea, DNA was extracted at a high yield. For both BSA and human γ-globulin, the proteins tested, the yield of DNA was high (some proteins in specimens may precipitate, but the precipitation can be managed with dilution or proteolytic enzymes5)). The aforementioned results show that the kit can extract residual DNA from various samples at high yields in a highly reproducible manner.

* Threshold® Total DNA assay system is manufactured by Molecular Devices for total DNA quantification and designed to quantitatively measure DNA as a molecule rather than as a gene. This system has a detection sensitivity of 2 pg/assay for total DNA.

Example of extraction of traces of residual DNA with DNA Extractor® Kit and DNA quantification by qPCR assay

As mentioned above, Threshold® Total DNA assay system is intended to quantify total DNA, but not to detect any specific gene. Considering the advantages of qPCR quantification for detecting a specific gene, which can be used to detect residual DNA clearly derived from host cells more sensitively than total DNA quantification with Threshold® Total DNA assay system, it was investigated whether traces of DNA could be extracted from potential host cells.

In this experiment, genomic DNA from CHO cells or Escherichia coli, which are widely used to produce proteins and antibodies, was used as a residual DNA model to extract and quantify traces of residual DNA using a qPCR assay. The experimental procedure is presented in Figure 1.

Figure 1. Experimental procedure

wb021698_img01.png

First, DNA was extracted from samples spiked with 0.1 to 100 pg of genomic DNA in 100 μL of water (distilled water for injection) using the kit and then dissolved in 100 μL of water. Subsequently, a qPCR assay was performed to calculate the yield of DNA from a calibration curve prepared simultaneously. The yield of DNA was 85% to 120% for Escherichia coligenome in the range of 1 to 100 pg and for CHO cell genome in the range of 0.1 to 100 pg (the yield was almost 100% up to 1,000 pg for both genomes, although no data are presented here).

Another experiment was performed based on the assumption that residual DNA was contained in a cell culture supernatant. After Panc-1, a human pancreatic cancer-derived cell line, was cultured in 10% FBS DMEM for 3 days, 0.1 pg of genomic DNA from CHO cells was added to 500 μL of culture supernatant to prepare a cell culture supernatant sample. The yield was calculated to be 0.093 pg from a calibration curve. This experiment showed that 0.1 pg (100 fg) of residual DNA in a 500-μL sample, as small as trace in fg units, can be extracted at a high yield using the kit. In addition, traces of residual DNA was recovered from all samples, including water, phosphate-buffered saline, and cell culture supernatant, at as high yields as almost 90% or more (Tables 1 and 2), suggesting that the kit can extract traces of residual DNA from various samples at high yields, as mentioned in the section of total DNA quantification.

Table 2. Yields of genomic DNA
Sample
(added genomic DNA)
Amount of DNA Amount of recovered DNA determined from calibration curve Yield
Distilled water for injection
(Escherichia coli)
0.1pg ND (less than lower limit of detection)
1pg 1.031pg 103%
10pg 11.09pg 111%
100pg 85.1pg 85%
Distilled water for injection
(CHO cells)
0.1pg 0.933pg 93%
1pg 1.187pg 119%
10pg 11.57pg 116%
100pg 87.1pg 87%
Human cell culture supernatant (CHO cells) 0.1pg 0.0928pg 93%

Conclusion

Residual DNA was recovered from samples at high yields using the kit. DNA extraction from samples is a very important step for quantifying residual DNA, and the kit can extract traces of residual DNA at high yields. In addition, since the kit can be used in various samples and may be useful in extracting residual DNA not only from CHO cells but also from other host cells such as Escherichia coli and yeast, the kit is expected to be used for testing of biopharmaceuticals in future.

References  参考文献

  1. Knezevic et al, Biologicals 36:203-211 (2008)
  2. Ishizawa, M et al, Nucleic Acids Res., 19, 5792 (1991)
  3. Kung. V. T et al, Anal. Biochem. 187, 220-227 (1990)
  4. Mizusawa S, Honma R, Pharm Tech. Japan 7, 309-314, 426-431 (1991)
  5. Wako Pure Chemicals Jiho Vol. 61 No. 1 p. 27 (1993)

1.Related product
DNA Extractor® KitRelated Products
DNA Extractor® Kit Series

Wako 019-19741小胶质细胞/巨噬细胞特异性抗体Anti Iba1, Rabbit

Wako 019-19741小胶质细胞/巨噬细胞特异性抗体Anti Iba1, Rabbit

离子钙接头蛋白分子1抗体(Anti Iba1, Rabbit)以合成的的Iba1羧基末端序列为抗原,该序列是人,大鼠和小鼠Iba1蛋白质保守序列。本抗体(免疫组化用)特异性与小胶质细胞/巨噬细胞反应,免疫双重染色与单克隆抗体结合,可与GFAP单克隆抗体((胶质纤维酸性蛋白,Glial Fibrillary Acidic Protein)联合使用对脑组织和细胞培养物进行双重染色。
名词解释:
Iba1: ionized calcium binding adapter molecule 1小胶质细胞/巨噬细胞特异性蛋白抗体
钙离子是已知的所有细胞,包括中枢神经系统(CNS)细胞中最重要的信号调解人之一。钙离子通过与各种钙结合蛋白结合发挥信号活动,其中许多钙结合蛋白被划分成一个大的蛋白家族,EF手性蛋白家族。Iba1是一个17 kDa的EF手性蛋白,在巨噬细胞和小胶质细胞中表达,并在这些细胞的活化过程中表达升高。
产品特点
S特异性检测小胶质细胞与/巨噬细胞,不与神经细胞与星形胶质细胞发生交叉反应。
可与人,大鼠,小鼠的Iba1反应
货号
品名
包装
用途
工作浓度
019-19741
Anti Iba1 polyclonal antibody, Rabbit, for Immunocytochemistry
50ug(100ul)
IH
1-2ug/ml
016-20001
Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting
50ug(100ul)
WB
0.5-1ug/ml
应用
石蜡切片应用方法:离子钙接头蛋白分子1抗体(Anti Iba1, Rabbit)
1. 固定
2. 用0.01M PBS缓冲液清洗二次
3. 使用含有0.3% 过氧化氢的甲醇孵育30分钟
4. 用0.01M PBS缓冲液清洗三次
5. 使用封闭液(含有1.5%的山羊血清和1%BSA的0.001M PBS缓冲液)室温孵育2小时
6. 使用含有Anti Iba1 polyclonal antibody的封闭液4℃孵育过夜
7. 用0.01M PBS缓冲液清洗三次
8. 使用含有生物素标记的抗兔IgG(1:200)的封闭液室温孵育1小时
9. 用0.01M PBS缓冲液清洗三次
10. 使用Elite ABC试剂(含有试剂A1:50,试剂B1:50的0.01M PBS溶液)
11. 用0.01M PBS缓冲液清洗三次
12. 过氧化物酶溶液(含有0.01% 过氧化氢和0.05% DAB的0.05M Tris溶液)
品牌 产品编号

(生产商编号)

产品名称 等级 规格
Wako
和光纯药
016-20001 Anti Iba1,Rabbit (for Western Blotting)

小胶质细胞/巨噬细胞特异性蛋白抗体(免疫印迹)

for Immunochemistry 50 ug
Wako
和光纯药
019-19741 Anti Iba1, Rabbit (for Immunocytochemistry)

小胶质细胞/巨噬细胞特异性蛋白抗体(免疫组化)

for Immunochemistry 50 ug
Wako
和光纯药
013-27593 Anti Human Iba1, Monoclonal Antibody(NCNP27)

抗人 Iba1,单抗(NCNP27)

for Immunochemistry 50 ul
Wako
和光纯药
017-27591 Anti Human Iba1, Monoclonal Antibody(NCNP27)

抗人 Iba1,单抗(NCNP27)

for Immunochemistry 10 ul
Wako
和光纯药
012-26723 Anti Iba1, Monoclonal Antibody(NCNP24)

抗Iba1,单克隆抗体(NCNP24)(鼠源)

for Immunochemistry 10 ul
Wako
和光纯药
016-26721 Anti Iba1, Monoclonal Antibody(NCNP24)

抗Iba1,单克隆抗体(NCNP24)(鼠源)

for Immunochemistry 50 ul
Wako
和光纯药
016-26461 Anti Iba1, Rabbit, Biotin-conjugated

小胶质细胞/巨噬细胞特异性蛋白抗体(结合生物素)

for Immunochemistry 100 ul
Wako
和光纯药
013-26471 Anti Iba1, Rabbit, Red Fluorochrome(635)-conjugated

小胶质细胞/巨噬细胞特异性蛋白抗体(结合红色荧光素635)

for Immunochemistry 100 ul

上海金畔生物科技有限公司(www.jinpanbio.cn)提供生命科学研究领域系列产品,包括生化试剂、色谱标准品和实验仪器耗材。主营Lumiprobe Cy系列活性荧光染料;WAKO日本和光纯药、修饰性PEG(Laysan bio、NANOCS、Avanti等品牌PEG以及定制合成修饰性聚乙二醇、单分散小分量PEG);Sigma、Amresco、TCI、MP bio生化试剂;日本关东化学Kanto试剂、日本三菱、日本柴田科学SIBATA;Megazyme食品分析检测试剂盒、日本共立理化学;Harlan饲料、Bio-Serv、日本CLEA Japan品牌的动物饲料;Oxoid、Nissui日水、日本荣研、BD difco、Himedia品牌微生物培养基;免疫试剂包括:Bethyl抗体;Biolegend流式抗体、Abcam、CST、Santa Cruz抗体;Roche、TOYOBO、NEB品牌的酶;中检所、TRC、药典USP、EP、Reagecon标准品;耗材和仪器包括Whatman、日本Advantec滤膜、Millipore品牌的各种滤膜、滤器和柱子填料等、Hampton蛋白结晶试剂耗材、小鼠软管灌胃针、动物毛发记号笔、Labnet、Wheaton瓶子、Bio-Rad伯乐、康宁Corning、Axygen、Falcon 、Eppendorf、Nunc、Nalgene、Nest品牌的培养皿、培养板、离心机、离心管、移液枪及枪头等实验室常用仪器耗材。

服务热线:18301939375   QQ:3258089810    3259632176

Wako 290-35591 MagCapture™ 系列用磁珠捕获磁力架

MagCapture™ 系列用磁珠捕获磁力架

● 可移动型弹起式的微管架固定装置。

● 可同时进行16支微管的磁珠捕获操作,可移液。

● 采用钕制磁石,缩减捕获时间。

◆概述

  本产品是用于捕获磁珠的磁性(力)支架。主要适用于MagCapture 系列对细胞培养上清、血清、尿液等样本含有的特定成分纯化而进行的磁珠法。

  可同时进行16支1.5 mL(0.2 mL)微管的操作,因配置强力磁石,可短时间内捕获微小的磁珠。

 

◆特点

● 可移动的弹起式微管架固定部。

● 弹起式装置装卸试管,可同时对16支微管进行磁珠捕获。

● 通过变化微管固定装置的角度可进行高效搅拌和灵活捕获。

6.jpg

● 采用钕制磁石,直接接触微管的构造,缩减捕获时间。

● 采用树脂材质,样本的可见性良好,实现了小型轻量化。

 

◆使用磁性(力)支架的应用案例

案例1.MagCapture Exosome Isolation Kit PS

  本试剂盒通过亲和法简便地从细胞培养上清和血清等样本中获得高纯度外泌体。通过外泌体膜表面存在的磷脂酰丝氨酸(PS)在金属离子条件下与Tim4蛋白结合,再经螯合剂处理获得完整状态的外泌体。使用磁力架可纯化高纯度外泌体。

5.jpg

◆方法

磁珠捕获时间 1.0 μm磁珠 1 mL:约25秒
2.7 μm磁珠 1 mL:约10秒
4.5 μm磁珠 1 mL:约2秒
作业容量 20 μL~1,500 μL(2,000 μL)
产品尺寸 W198.8×D49×H49(mm)
重量 235 g
产品编号 产品名称 产品规格 产品等级 产品价格
290-35591 MagCapture™ 系列磁珠捕获用磁力架 Magnet Stand 1个

Wako 分散酶 Dispase CAS 9001-92-7

Wako 分散酶 Dispase CAS 9001-92-7

Dispase分散酶

Dispase分散酶是一类中性金属蛋白水解酶,可以回收生长在Matrigel基质上的细胞。与胰酶、胶原酶相比,它的作用更加温和,所以不会损伤细胞。此外,分散酶也可以用于组织分离。

来源:多粘芽孢杆菌表达的金属蛋白酶
使用指南:推荐浓度为10 U/cm2 BD Matrigel基质,如35 mm的培养皿推荐使用浓度为100U。
保存条件和效期:-20℃下冷冻保存,避免多次冻融。保质期详见产品包装。

Wako 分散酶 Dispase CAS 9001-92-7

383-02281 DISPASEⅡ 分散酶Ⅱ 1 g 9001-92-7
386-02271 DISPASE®Ⅰ 分散酶 10000 PU×6 9001-92-7

Roche公司的Dispase分散酶产品

货号 品名 规格
Roche-04942078001 Dispase® II (neutral protease, grade II) 1 g
Roche-04942078001 Dispase® II (neutral protease, grade II) 100mg

\

Wako和光纯药抑制剂 Inhibitor

Wako和光纯药抑制剂 Inhibitor

上海金畔生物代理Wako和光纯药全线产品,欢迎新老客户访问Wako和光纯药官网或者咨询我们获取更多相关产品信息。

Wako和光纯药代理

货号 品名 规格
307-50771 α-Amylase Inhibitor, From Wheat  α-淀粉酶抑制剂,来源于小麦 1MG
335-40623 Antipain 25MG
333-40624 Antipain 100MG
339-40621 Antipain 0.5MG
253-00471 YC-1 5MG
244-00721 Xestospongin C 100UG
210-00921 U-73343 5MG
213-00911 U-73122 5MG
211-01051 U0126 5MG
208-09223 Trypsin Inhibitor, from Soybean
胰蛋白酶抑制剂,大豆来源
1G
204-09225 Trypsin Inhibitor, from Soybean
胰蛋白酶抑制剂,大豆来源
5G
202-09226 Trypsin Inhibitor, from Soybean
胰蛋白酶抑制剂,大豆来源
500MG
202-09221 Trypsin Inhibitor, from Soybean
胰蛋白酶抑制剂,大豆来源
100MG
204-11991 Trichostatin A
曲古柳菌素A
5MG
200-11993 Trichostatin A
曲古柳菌素A
1MG
206-15471 Trichodion 100UG
209-14481 N-Tosyl-L-phenylalanine Chloromethyl Ketone(TPCK) 250MG
205-14483 N-Tosyl-L-phenylalanine Chloromethyl Ketone(TPCK) 1G
208-09181 (+/-)-alpha-Tocopherol Nicotinate
alpha-维生素E烟酸酯
5G
206-09182 (+/-)-alpha-Tocopherol Nicotinate
alpha-维生素E烟酸酯
25G
207-15641 TMP-153 500MG
164-17321 DL-threo-PPMP Hydrochloride
D,L-苏式-PPMP盐酸盐
25MG
161-17331 DL-threo-PPMP Hydrochloride
D,L-苏式-PPMP盐酸盐
50MG
206-10351 1-(2-Tetrahydrofuryl)-5-fluorouracil
1-(2-四氢呋喃基)-5-氟尿嘧啶
1G
202-10353 1-(2-Tetrahydrofuryl)-5-fluorouracil
1-(2-四氢呋喃基)-5-氟尿嘧啶
5G
209-12041 Tautomycin
泰托霉素
100UG
198-10281 Swainsonine
八倾吲嗪三醇
1MG
196-12703 Sulindac
舒林酸
50G
190-12701 Sulindac
舒林酸
10G
195-12491 Sulfuretin
硫黄菊素
20MG
333-31091 SUC-GLY-PRO-MCA 5MG
549-00286 STREPTOZOTOCIN 5G
543-00284 STREPTOZOTOCIN 1G
545-00283 STREPTOZOTOCIN 500MG
549-00281 STREPTOZOTOCIN 100MG
198-08515 Streptomycin Sulfate
硫酸链霉素
500G
192-08513 Streptomycin Sulfate
硫酸链霉素
100G
194-08512 Streptomycin Sulfate
硫酸链霉素
25G
196-08511 Streptomycin Sulfate
硫酸链霉素
5G
197-10251 Staurosporine
星形孢菌素
100UG
193-10253 Staurosporine
星形孢菌素
500UG
191-11533 Spectinomycin Dihydrochloride Pentahydrate
盐酸壮观霉素
1G
195-11531 Spectinomycin Dihydrochloride Pentahydrate
盐酸壮观霉素
5G
193-12051 Simvastatin
新伐他丁
25MG
194-13561 γ-Secretase Inhibitor X 1MG
188-01593 Roscovitine, 98.0+ % (HPLC)
细胞周期蛋白B激酶抑制剂
10MG
182-01591 Roscovitine, 98.0+ % (HPLC)
细胞周期蛋白B激酶抑制剂
1MG
181-01723 Resveratrol
白藜芦醇
500MG
185-01721 Resveratrol
白藜芦醇
100MG
183-01901 Radicicol
根赤壳菌素
1MG
165-20281 Protease Inhibitor Mixture-DMSO Solution for Fungal and Yeast Extracts 1ML
161-11493 Prednisolone
强的松龙
5G
165-11491 Prednisolone
强的松龙
1G
162-19821 Pravastatin Sodium Salt
普伐他汀
25MG
168-19823 Pravastatin Sodium Salt
普伐他汀
100MG
163-20341 PP-3
PP3酪氨酸激酶抑制剂
1MG
166-20331 PP 2, 94.0+ % (HPLC) 1MG
166-20294 Piroxicam
炎痛喜康
10G
162-20291 Piroxicam
炎痛喜康
1G
168-20293 Piroxicam
炎痛喜康
5G
160-17781 Phloretin, 98.0+ % (HPLC)
根皮素
250MG
160-11181 Pepsinostreptin
抑胃酶素
10MG
163-20101 PACOCF3
1,1,1-三氟-2-十七烷酮
10MG
144-07331 NS-398
COX-2选择性抑制剂NS-398
5MG
140-07333 NS-398
COX-2选择性抑制剂NS-398
25MG
147-07343 Niflumic Acid
尼氟酸
250G
141-07341 Niflumic Acid
尼氟酸
50G
145-06381 Nicardipine Hydrochloride, 99.0+ % (HPLC)
盐酸尼卡地平
1G
141-06383 Nicardipine Hydrochloride, 99.0+ % (HPLC)
盐酸尼卡地平
5G
145-06761 NF-κB inhibitor SN50 500UG
147-07201 (S)-(+)-Naproxen
(S)-(+)-萘普生
5G

Wako 074-06161 Glutelin, from Ric

Wako 074-06161 Glutelin, from Ric

Glutelin, from Rice(Yamadanishiki)
级别:for Cellbiology
代理商 : FUJIFILM Wako Pure Chemical Corporation
保存条件Storage Condition : Keep at 2-10 degrees C.

This product is for research use only. Do not administer it to human.

Glutelin is a major protein in rice, and defined as protein which dissolves in dilute acid and alkali.
In Japanese sake brewing, the byproducts of protein breakdown are considered to be a decisive factor in establishing flavor. The National Tax Agency of Japan has determined a special method to measure carboxypeptidase activity in koji mold (Aspergillus oryzae) used for fermentation. However, in recent years it has been reported that synthetic peptide substrates approved by this test are impractical in producing enzymatic activity from koji mold. To this extent, gluterin derived from rice has been shown to be an ideal solution to this problem.